Detection of nucleic acid sequences by invader-directed cleavage

Brow, Mary Ann D; Hall, Jeff Steven Grotelueschen; Lyamichev, Victor; Olive, David Michael; Prudent, James Robert

Detection of nucleic acid sequences by invader-directed cleavage

Patent · Fri Jan 01 04:00:00 EST 1999

OSTI ID:872754

Brow, Mary Ann D ^[1]; Hall, Jeff Steven Grotelueschen ^[1]; Lyamichev, Victor ^[1]; Olive, David Michael ^[1]; Prudent, James Robert ^[1]

Madison, WI

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The 5' nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based by charge.

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Research Organization:: Third Wave Technologies, Inc., Madison, WI (United States)

DOE Contract Number:: FG02-94ER81891

Assignee:: Third Wave Technologies, Inc. (CA)

Patent Number(s):: US 6001567

OSTI ID:: 872754

Country of Publication:: United States

Language:: English

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Detection of nucleic acid sequences by invader-directed cleavage

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