Detection of nucleic acid sequences by invader-directed cleavage
Patent
·
OSTI ID:20013254
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The 5{prime} nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based by charge.
- Research Organization:
- US Department of Energy, Washington, DC (US)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG02-94ER81891
- Assignee:
- PTO; EDB-00:006676
- Application Number:
- Int. Cl. C12Q 1/68; C12P 19/34; C07H 21/02; US 6,001,567/A/; 8-682,853; TRN: IM200010%%122
- OSTI ID:
- 20013254
- Resource Relation:
- Other Information: PBD: 14 Dec 1999
- Country of Publication:
- United States
- Language:
- English
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