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Title: Complete Genome Sequence of Dehalobacterium formicoaceticum Strain DMC, a Strictly Anaerobic Dichloromethane-Degrading Bacterium

Abstract

ABSTRACT Dehalobacterium formicoaceticumutilizes dichloromethane as the sole energy source in defined anoxic bicarbonate-buffered mineral salt medium. The products are formate, acetate, inorganic chloride, and biomass. The bacterium’s genome was sequenced using PacBio, assembled, and annotated. The complete genome consists of one 3.77-Mb circular chromosome harboring 3,935 predicted protein-encoding genes.

Authors:
 [1];  [2];  [3];  [4]; ORCiD logo [5]
  1. Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Civil and Environmental Engineering
  2. Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology
  3. E. I. DuPont de Nemours and Company, Newark, DE (United States). Corporate Remediation Group
  4. Chemours Company, Wilmington, DE (United States)
  5. Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Civil and Environmental Engineering; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Microbiology; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Biosystems Engineering and Soil Science; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1399397
Alternate Identifier(s):
OSTI ID: 1470849
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Genome Announcements
Additional Journal Information:
Journal Volume: 5; Journal Issue: 37; Journal ID: ISSN 2169-8287
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Chen, Gao, Murdoch, Robert W., Mack, E. Erin, Seger, Edward S., and Loeffler, Frank E. Complete Genome Sequence of Dehalobacterium formicoaceticum Strain DMC, a Strictly Anaerobic Dichloromethane-Degrading Bacterium. United States: N. p., 2017. Web. doi:10.1128/genomeA.00897-17.
Chen, Gao, Murdoch, Robert W., Mack, E. Erin, Seger, Edward S., & Loeffler, Frank E. Complete Genome Sequence of Dehalobacterium formicoaceticum Strain DMC, a Strictly Anaerobic Dichloromethane-Degrading Bacterium. United States. https://doi.org/10.1128/genomeA.00897-17
Chen, Gao, Murdoch, Robert W., Mack, E. Erin, Seger, Edward S., and Loeffler, Frank E. Thu . "Complete Genome Sequence of Dehalobacterium formicoaceticum Strain DMC, a Strictly Anaerobic Dichloromethane-Degrading Bacterium". United States. https://doi.org/10.1128/genomeA.00897-17. https://www.osti.gov/servlets/purl/1399397.
@article{osti_1399397,
title = {Complete Genome Sequence of Dehalobacterium formicoaceticum Strain DMC, a Strictly Anaerobic Dichloromethane-Degrading Bacterium},
author = {Chen, Gao and Murdoch, Robert W. and Mack, E. Erin and Seger, Edward S. and Loeffler, Frank E.},
abstractNote = {ABSTRACT Dehalobacterium formicoaceticumutilizes dichloromethane as the sole energy source in defined anoxic bicarbonate-buffered mineral salt medium. The products are formate, acetate, inorganic chloride, and biomass. The bacterium’s genome was sequenced using PacBio, assembled, and annotated. The complete genome consists of one 3.77-Mb circular chromosome harboring 3,935 predicted protein-encoding genes.},
doi = {10.1128/genomeA.00897-17},
journal = {Genome Announcements},
number = 37,
volume = 5,
place = {United States},
year = {Thu Sep 14 00:00:00 EDT 2017},
month = {Thu Sep 14 00:00:00 EDT 2017}
}

Works referenced in this record:

Versatile and open software for comparing large genomes
journal, January 2004

  • Kurtz, Stefan; Phillippy, Adam; Delcher, Arthur L.
  • Genome Biology, Vol. 5, Issue 2, R12
  • DOI: 10.1186/gb-2004-5-2-r12

Bacterial growth with chlorinated methanes.
journal, June 1995

  • Leisinger, T.; Braus-Stromeyer, S. A.
  • Environmental Health Perspectives, Vol. 103, Issue suppl 5
  • DOI: 10.1289/ehp.95103s433

Identifying bacterial genes and endosymbiont DNA with Glimmer
journal, January 2007


The IGS Standard Operating Procedure for Automated Prokaryotic Annotation
journal, April 2011

  • Galens, Kevin; Orvis, Joshua; Daugherty, Sean
  • Standards in Genomic Sciences, Vol. 4, Issue 2
  • DOI: 10.4056/sigs.1223234

RNAmmer: consistent and rapid annotation of ribosomal RNA genes
journal, April 2007

  • Lagesen, Karin; Hallin, Peter; Rødland, Einar Andreas
  • Nucleic Acids Research, Vol. 35, Issue 9
  • DOI: 10.1093/nar/gkm160

Initial Characterization of a Reductive Dehalogenase from Desulfitobacterium chlororespirans Co23.
journal, January 1996


Canu: scalable and accurate long-read assembly via adaptive k -mer weighting and repeat separation
journal, March 2017

  • Koren, Sergey; Walenz, Brian P.; Berlin, Konstantin
  • Genome Research, Vol. 27, Issue 5
  • DOI: 10.1101/gr.215087.116

tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic Sequence
journal, March 1997


tRNAscan-SE: A Program for Improved Detection of Transfer RNA Genes in Genomic Sequence
journal, March 1997


Circleator: flexible circular visualization of genome-associated data with BioPerl and SVG
journal, July 2014


Dichloromethane utilized by an anaerobic mixed culture: acetogenesis and methanogenesis
journal, January 1991

  • Stromeyer, Susanna A.; Winkelbauer, Wolfgang; Kohler, Herbert
  • Biodegradation, Vol. 2, Issue 2
  • DOI: 10.1007/BF00114603

Enrichment, Cultivation, and Detection of Reductively Dechlorinating Bacteria
book, January 2005


The complete genome sequence of Moorella thermoacetica (f. Clostridium thermoaceticum)
journal, July 2008


‘ Candidatus Dichloromethanomonas elyunquensis’ gen. nov., sp. nov., a dichloromethane-degrading anaerobe of the Peptococcaceae family
journal, April 2017

  • Kleindienst, Sara; Higgins, Steven A.; Tsementzi, Despina
  • Systematic and Applied Microbiology, Vol. 40, Issue 3
  • DOI: 10.1016/j.syapm.2016.12.001

Metabolism of Dichloromethane by the Strict Anaerobe Dehalobacterium formicoaceticum
journal, January 1998

  • Mägli, Andreas; Messmer, Michael; Leisinger, Thomas
  • Applied and Environmental Microbiology, Vol. 64, Issue 2
  • DOI: 10.1128/AEM.64.2.646-650.1998

Bacterial Degradation of Dichloromethane
journal, January 1980


Dichloromethane dehalogenase of Hyphomicrobium sp. strain DM2.
journal, January 1985


Biodegradation of dichloromethane and its utilization as a growth substrate under methanogenic conditions.
journal, January 1991


Acetogenesis from dichloromethane by a two-component mixed culture comprising a novel bacterium.
journal, January 1995


Draft Genome Sequence of Medusavirus Stheno, Isolated from the Tatakai River of Uji, Japan
journal, January 2021

  • Yoshida, Koki; Zhang, Ruixuan; Garcia, Kimberly G.
  • Microbiology Resource Announcements, Vol. 10, Issue 1
  • DOI: 10.1128/mra.01323-20

Works referencing / citing this record:

Complete Genome Sequence of Dehalococcoides mccartyi Strain FL2, a Trichloroethene-Respiring Anaerobe Isolated from Pristine Freshwater Sediment
journal, August 2019

  • Yan, Jun; Yang, Yi; Li, Xiuying
  • Microbiology Resource Announcements, Vol. 8, Issue 33
  • DOI: 10.1128/mra.00558-19

‘NetShift’: a methodology for understanding ‘driver microbes’ from healthy and disease microbiome datasets
journal, October 2018


Proteogenomics Reveals Novel Reductive Dehalogenases and Methyltransferases Expressed during Anaerobic Dichloromethane Metabolism
journal, January 2019

  • Kleindienst, Sara; Chourey, Karuna; Chen, Gao
  • Applied and Environmental Microbiology, Vol. 85, Issue 6
  • DOI: 10.1128/aem.02768-18