Dual Carbon–Chlorine Isotope Analysis Indicates Distinct Anaerobic Dichloromethane Degradation Pathways in Two Members of Peptococcaceae
Abstract
Dichloromethane (DCM) is a probable human carcinogen and frequent groundwater contaminant and contributes to stratospheric ozone layer depletion. DCM is degraded by aerobes harboring glutathione-dependent DCM dehalogenases; however, DCM contamination occurs in oxygen-deprived environments, and much less is known about anaerobic DCM metabolism. Some members of the Peptococcaceae family convert DCM to environmentally benign products including acetate, formate, hydrogen (H2), and inorganic chloride under strictly anoxic conditions. The current study applied stable carbon and chlorine isotope fractionation measurements to the axenic culture Dehalobacterium formicoaceticum and to the consortium RM comprising DCM degrader Candidatus Dichloromethanomonas elyunquensis. Degradation-associated carbon and chlorine isotope enrichment factors (εC and εCl) of -42.4 ± 0.7‰ and -5.3 ± 0.1‰, respectively, were measured in D. formicoaceticum cultures. A similar εCl of -5.2 ± 0.1‰, but a substantially lower εC of -18.3 ± 0.2‰, were determined for Ca. Dichloromethanomonas elyunquensis. The εC and εCl values resulted in distinctly different dual element C–Cl isotope correlations (ΛC/Cl = Δδ13C/Δδ37Cl) of 7.89 ± 0.12 and 3.40 ± 0.03 for D. formicoaceticum and Ca. Dichloromethanomonas elyunquensis, respectively. The distinct ΛC/Cl values obtained for the two cultures imply mechanistically distinct C–Cl bond cleavage reactions, suggesting that members of Peptococcaceae employ different pathways tomore »
- Authors:
-
- Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology, Dept. of Civil and Environmental Engineering, and Dept. of Microbiology
- Isotope Tracer Technologies Inc. (IT2), Waterloo, Ontario (Canada); Univ. of Waterloo, Waterloo, Ontario (Canada). Dept. of Earth and Environmental Sciences; Univ. of Guelph, Guelph, Ontario (Canada). School of Engineering,
- Univ. of Toronto, Toronto, Ontario (Canada). Dept. of Earth Sciences
- Georgia Inst. of Technology, Atlanta, GA (United States). School of Biology
- DuPont de Nemours and Company, Wilmington, DE (United States). DuPont Corporate Remediation Group
- The Chemours Company, Wilmington, DE (United States)
- Univ. of Tennessee, Knoxville, TN (United States). Center for Environmental Biotechnology, Dept. of Civil and Environmental Engineering, and Dept. of Microbiology; Univ. of TN and Oak Ridge National Lab., Oak Ridge, TN (United States). Oak Ridge National Laboratory (UT-ORNL) Joint Institute for Biological Sciences (JIBS) and Biosciences Division; University of Tennessee, Knoxville, TN (United States). Dept. of Biosystems Engineering and Soil Science
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1474553
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Science and Technology
- Additional Journal Information:
- Journal Volume: 52; Journal Issue: 15; Journal ID: ISSN 0013-936X
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES
Citation Formats
Chen, Gao, Shouakar-Stash, Orfan, Phillips, Elizabeth, Justicia-Leon, Shandra D., Gilevska, Tetyana, Sherwood Lollar, Barbara, Mack, E. Erin, Seger, Edward S., and Löffler, Frank E. Dual Carbon–Chlorine Isotope Analysis Indicates Distinct Anaerobic Dichloromethane Degradation Pathways in Two Members of Peptococcaceae. United States: N. p., 2018.
Web. doi:10.1021/acs.est.8b01583.
Chen, Gao, Shouakar-Stash, Orfan, Phillips, Elizabeth, Justicia-Leon, Shandra D., Gilevska, Tetyana, Sherwood Lollar, Barbara, Mack, E. Erin, Seger, Edward S., & Löffler, Frank E. Dual Carbon–Chlorine Isotope Analysis Indicates Distinct Anaerobic Dichloromethane Degradation Pathways in Two Members of Peptococcaceae. United States. https://doi.org/10.1021/acs.est.8b01583
Chen, Gao, Shouakar-Stash, Orfan, Phillips, Elizabeth, Justicia-Leon, Shandra D., Gilevska, Tetyana, Sherwood Lollar, Barbara, Mack, E. Erin, Seger, Edward S., and Löffler, Frank E. Thu .
"Dual Carbon–Chlorine Isotope Analysis Indicates Distinct Anaerobic Dichloromethane Degradation Pathways in Two Members of Peptococcaceae". United States. https://doi.org/10.1021/acs.est.8b01583. https://www.osti.gov/servlets/purl/1474553.
@article{osti_1474553,
title = {Dual Carbon–Chlorine Isotope Analysis Indicates Distinct Anaerobic Dichloromethane Degradation Pathways in Two Members of Peptococcaceae},
author = {Chen, Gao and Shouakar-Stash, Orfan and Phillips, Elizabeth and Justicia-Leon, Shandra D. and Gilevska, Tetyana and Sherwood Lollar, Barbara and Mack, E. Erin and Seger, Edward S. and Löffler, Frank E.},
abstractNote = {Dichloromethane (DCM) is a probable human carcinogen and frequent groundwater contaminant and contributes to stratospheric ozone layer depletion. DCM is degraded by aerobes harboring glutathione-dependent DCM dehalogenases; however, DCM contamination occurs in oxygen-deprived environments, and much less is known about anaerobic DCM metabolism. Some members of the Peptococcaceae family convert DCM to environmentally benign products including acetate, formate, hydrogen (H2), and inorganic chloride under strictly anoxic conditions. The current study applied stable carbon and chlorine isotope fractionation measurements to the axenic culture Dehalobacterium formicoaceticum and to the consortium RM comprising DCM degrader Candidatus Dichloromethanomonas elyunquensis. Degradation-associated carbon and chlorine isotope enrichment factors (εC and εCl) of -42.4 ± 0.7‰ and -5.3 ± 0.1‰, respectively, were measured in D. formicoaceticum cultures. A similar εCl of -5.2 ± 0.1‰, but a substantially lower εC of -18.3 ± 0.2‰, were determined for Ca. Dichloromethanomonas elyunquensis. The εC and εCl values resulted in distinctly different dual element C–Cl isotope correlations (ΛC/Cl = Δδ13C/Δδ37Cl) of 7.89 ± 0.12 and 3.40 ± 0.03 for D. formicoaceticum and Ca. Dichloromethanomonas elyunquensis, respectively. The distinct ΛC/Cl values obtained for the two cultures imply mechanistically distinct C–Cl bond cleavage reactions, suggesting that members of Peptococcaceae employ different pathways to metabolize DCM. These findings emphasize the utility of dual carbon–chlorine isotope analysis to pinpoint DCM degradation mechanisms and to provide an additional line of evidence that detoxification is occurring at DCM-contaminated sites.},
doi = {10.1021/acs.est.8b01583},
journal = {Environmental Science and Technology},
number = 15,
volume = 52,
place = {United States},
year = {Thu Jul 05 00:00:00 EDT 2018},
month = {Thu Jul 05 00:00:00 EDT 2018}
}
Web of Science
Works referenced in this record:
Stable Carbon Isotope Enrichment Factors for cis -1,2-Dichloroethene and Vinyl Chloride Reductive Dechlorination by Dehalococcoides
journal, April 2011
- Fletcher, Kelly E.; Nijenhuis, Ivonne; Richnow, Hans-Hermann
- Environmental Science & Technology, Vol. 45, Issue 7
A review of bacterial methyl halide degradation: biochemistry, genetics and molecular ecology
journal, April 2002
- McDonald, I. R.; Warner, K. L.; McAnulla, C.
- Environmental Microbiology, Vol. 4, Issue 4
Guidelines and recommended terms for expression of stable-isotope-ratio and gas-ratio measurement results: Guidelines and recommended terms for expressing stable isotope results
journal, August 2011
- Coplen, Tyler B.
- Rapid Communications in Mass Spectrometry, Vol. 25, Issue 17
Substrate Hydrophobicity and Cell Composition Influence the Extent of Rate Limitation and Masking of Isotope Fractionation during Microbial Reductive Dehalogenation of Chlorinated Ethenes
journal, March 2015
- Renpenning, Julian; Rapp, Insa; Nijenhuis, Ivonne
- Environmental Science & Technology, Vol. 49, Issue 7
Bacterial growth with chlorinated methanes.
journal, June 1995
- Leisinger, T.; Braus-Stromeyer, S. A.
- Environmental Health Perspectives, Vol. 103, Issue suppl 5
Extending Limits of Chlorine Kinetic Isotope Effects
journal, May 2012
- Świderek, Katarzyna; Paneth, Piotr
- The Journal of Organic Chemistry, Vol. 77, Issue 11
Influence of Mass-Transfer Limitations on Carbon Isotope Fractionation during Microbial Dechlorination of Trichloroethene
journal, October 2009
- Aeppli, Christoph; Berg, Michael; Cirpka, Olaf A.
- Environmental Science & Technology, Vol. 43, Issue 23
Influence of mass transfer on stable isotope fractionation
journal, November 2012
- Thullner, Martin; Fischer, Anko; Richnow, Hans-Hermann
- Applied Microbiology and Biotechnology, Vol. 97, Issue 2
Large Carbon Isotope Fractionation during Biodegradation of Chloroform by Dehalobacter Cultures
journal, August 2012
- Chan, Calvin C. H.; Mundle, Scott O. C.; Eckert, Thomas
- Environmental Science & Technology
Carbon and Chlorine Isotope Fractionation Patterns Associated with Different Engineered Chloroform Transformation Reactions
journal, May 2017
- Torrentó, Clara; Palau, Jordi; Rodríguez-Fernández, Diana
- Environmental Science & Technology, Vol. 51, Issue 11
Hydrogen Isotope Fractionation As a Tool to Identify Aerobic and Anaerobic PAH Biodegradation
journal, February 2016
- Kümmel, Steffen; Starke, Robert; Chen, Gao
- Environmental Science & Technology, Vol. 50, Issue 6
Reductive Outer-Sphere Single Electron Transfer Is an Exception Rather than the Rule in Natural and Engineered Chlorinated Ethene Dehalogenation
journal, August 2017
- Heckel, Benjamin; Cretnik, Stefan; Kliegman, Sarah
- Environmental Science & Technology, Vol. 51, Issue 17
Measurement of Henry's law constants for C1 and C2 chlorinated hydrocarbons
journal, February 1987
- Gossett, James M.
- Environmental Science & Technology, Vol. 21, Issue 2
Large carbon isotope fractionation associated with oxidation of methyl halides by methylotrophic bacteria
journal, May 2001
- Miller, L. G.; Kalin, R. M.; McCauley, S. E.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 10
Compound-specific chlorine isotope ratios of TCE, PCE and DCE isomers by direct injection using CF-IRMS
journal, May 2006
- Shouakar-Stash, Orfan; Drimmie, Robert J.; Zhang, Min
- Applied Geochemistry, Vol. 21, Issue 5
Stable isotope fractionation to investigate natural transformation mechanisms of organic contaminants: principles, prospects and limitations
journal, January 2010
- Elsner, Martin
- Journal of Environmental Monitoring, Vol. 12, Issue 11
Isolation and characterization of Dehalobacterium formicoaceticum gen. nov. sp. nov., a strictly anaerobic bacterium utilizing dichloromethane as source of carbon and energy
journal, August 1996
- Mägli, Andreas; Wendt, Matthias; Leisinger, T.
- Archives of Microbiology, Vol. 166, Issue 2
Hydrogen and carbon isotope fractionation during degradation of chloromethane by methylotrophic bacteria
journal, September 2013
- Nadalig, Thierry; Greule, Markus; Bringel, Françoise
- MicrobiologyOpen, Vol. 2, Issue 6
‘ Candidatus Dichloromethanomonas elyunquensis’ gen. nov., sp. nov., a dichloromethane-degrading anaerobe of the Peptococcaceae family
journal, April 2017
- Kleindienst, Sara; Higgins, Steven A.; Tsementzi, Despina
- Systematic and Applied Microbiology, Vol. 40, Issue 3
New Evaluation Scheme for Two-Dimensional Isotope Analysis to Decipher Biodegradation Processes: Application to Groundwater Contamination by MTBE
journal, February 2005
- Zwank, Luc; Berg, Michael; Elsner, Martin
- Environmental Science & Technology, Vol. 39, Issue 4
Carbon and nitrogen isotope analysis of atrazine and desethylatrazine at sub-microgram per liter concentrations in groundwater
journal, December 2012
- Schreglmann, Kathrin; Hoeche, Martina; Steinbeiss, Sibylle
- Analytical and Bioanalytical Chemistry, Vol. 405, Issue 9
Relative Contributions of Dehalobacter and Zerovalent Iron in the Degradation of Chlorinated Methanes
journal, March 2015
- Lee, Matthew; Wells, Eliza; Wong, Yie Kuan
- Environmental Science & Technology, Vol. 49, Issue 7
Mutualistic interaction between dichloromethane- and chloromethane-degrading bacteria in an anaerobic mixed culture: Mutualistic degradation of chlorinated methanes
journal, October 2017
- Chen, Gao; Kleindienst, Sara; Griffiths, Daniel R.
- Environmental Microbiology, Vol. 19, Issue 11
A corrinoid-dependent catabolic pathway for growth of a Methylobacterium strain with chloromethane
journal, April 1999
- Vannelli, T.; Messmer, M.; Studer, A.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 8
Headspace Analysis: A New Application for Isotopic Characterization of Dissolved Organic Contaminants
journal, January 1999
- Slater, Gregory F.; Dempster, Helen S.; Sherwood Lollar, Barbara
- Environmental Science & Technology, Vol. 33, Issue 1
Dichloromethane-degrading bacteria in the genomic age
journal, November 2011
- Muller, Emilie E. L.; Bringel, Françoise; Vuilleumier, Stéphane
- Research in Microbiology, Vol. 162, Issue 9
Evaluating Pesticide Degradation in the Environment: Blind Spots and Emerging Opportunities
journal, August 2013
- Fenner, Kathrin; Canonica, Silvio; Wackett, Lawrence P.
- Science, Vol. 341, Issue 6147
The increasing threat to stratospheric ozone from dichloromethane
journal, June 2017
- Hossaini, Ryan; Chipperfield, Martyn P.; Montzka, Stephen A.
- Nature Communications, Vol. 8, Issue 1
Determination of Compound-Specific Carbon Isotope Ratios of Chlorinated Methanes, Ethanes, and Ethenes in Aqueous Samples
journal, July 2000
- Hunkeler, Daniel; Aravena, Ramon
- Environmental Science & Technology, Vol. 34, Issue 13
The Expression of Isotope Effects on Enzyme-Catalyzed Reactions
journal, June 1981
- Northrop, D. B.
- Annual Review of Biochemistry, Vol. 50, Issue 1
A New Concept Linking Observable Stable Isotope Fractionation to Transformation Pathways of Organic Pollutants
journal, September 2005
- Elsner, Martin; Zwank, Luc; Hunkeler, Daniel
- Environmental Science & Technology, Vol. 39, Issue 18
Stable Carbon Isotope Evidence for Intrinsic Bioremediation of Tetrachloroethene and Trichloroethene at Area 6, Dover Air Force Base
journal, January 2001
- Sherwood Lollar, B.; Slater, G. F.; Sleep, B.
- Environmental Science & Technology, Vol. 35, Issue 2
Stable Carbon Isotope Fractionation of 1,2-Dichloropropane during Dichloroelimination by Dehalococcoides Populations
journal, August 2009
- Fletcher, Kelly E.; Löffler, Frank E.; Richnow, Hans-Hermann
- Environmental Science & Technology, Vol. 43, Issue 18
Isotopic fractionation of carbon and chlorine by microbial degradation of dichloromethane
journal, August 1999
- Heraty, L. J.; Fuller, M. E.; Huang, L.
- Organic Geochemistry, Vol. 30, Issue 8
Rayleigh equation for evolution of stable isotope ratios in contaminant decay chains
journal, February 2014
- Höhener, Patrick; Atteia, Olivier
- Geochimica et Cosmochimica Acta, Vol. 126
Bacterial dehalogenases: biochemistry, genetics, and biotechnological applications.
journal, January 1994
- Fetzner, S.; Lingens, F.
- Microbiological Reviews, Vol. 58, Issue 4
Carbon and hydrogen stable isotope fractionation associated with the anaerobic degradation of propane and butane by marine sulfate-reducing bacteria: Degradation of gaseous alkanes - isotope fractionation
journal, September 2013
- Jaekel, Ulrike; Vogt, Carsten; Fischer, Anko
- Environmental Microbiology, Vol. 16, Issue 1
New guidelines for reporting stable hydrogen, carbon, and oxygen isotope-ratio data
journal, September 1996
- Coplen, Tyler B.
- Geochimica et Cosmochimica Acta, Vol. 60, Issue 17
A New Interpretation of Chlorine Leaving Group Kinetic Isotope Effects; A Theoretical Approach
journal, July 2004
- Dybała-Defratyka, Agnieszka; Rostkowski, Michał; Matsson, Olle
- The Journal of Organic Chemistry, Vol. 69, Issue 15
Complete chloroform dechlorination by organochlorine respiration and fermentation: Microbial chloroform metabolism
journal, November 2011
- Lee, Matthew; Low, Adrian; Zemb, Olivier
- Environmental Microbiology, Vol. 14, Issue 4
Isotopic Evidence Suggests Different Initial Reaction Mechanisms for Anaerobic Benzene Biodegradation
journal, November 2008
- Mancini, Silvia A.; Devine, Cheryl E.; Elsner, Martin
- Environmental Science & Technology, Vol. 42, Issue 22
Pressure-monitored headspace analysis combined with compound-specific isotope analysis to measure isotope fractionation in gas-producing reactions: Pressure-monitored headspace analysis and CSIA to measure ε values
journal, June 2013
- Mundle, Scott O. C.; Vandersteen, Adelle A.; Lacrampe-Couloume, Georges
- Rapid Communications in Mass Spectrometry, Vol. 27, Issue 15
Dichloromethane biodegradation in multi-contaminated groundwater: Insights from biomolecular and compound-specific isotope analyses
journal, October 2018
- Hermon, L.; Denonfoux, J.; Hellal, J.
- Water Research, Vol. 142
Impact of Bioavailability Restrictions on Microbially Induced Stable Isotope Fractionation. 2. Experimental Evidence
journal, July 2008
- Kampara, Makeba; Thullner, Martin; Richnow, Hans H.
- Environmental Science & Technology, Vol. 42, Issue 17
C and Cl Isotope Fractionation of 1,2-Dichloroethane Displays Unique δ 13 C/δ 37 Cl Patterns for Pathway Identification and Reveals Surprising C–Cl Bond Involvement in Microbial Oxidation
journal, July 2014
- Palau, Jordi; Cretnik, Stefan; Shouakar-Stash, Orfan
- Environmental Science & Technology, Vol. 48, Issue 16
Factors controlling the carbon isotope fractionation of tetra- and trichloroethene during reductive dechlorination by Sulfurospirillum ssp. and Desulfitobacterium sp. strain PCE-S: Carbon isotope fractionation during reductive dechlorination
journal, October 2007
- Cichocka, Danuta; Siegert, Michael; Imfeld, Gwenaël
- FEMS Microbiology Ecology, Vol. 62, Issue 1
Molecular and carbon isotopic characterization of an anaerobic stable enrichment culture containing Dehalobacterium sp. during dichloromethane fermentation
journal, March 2017
- Trueba-Santiso, Alba; Parladé, Eloi; Rosell, Mònica
- Science of The Total Environment, Vol. 581-582
Diffusion Sampler for Compound Specific Carbon Isotope Analysis of Dissolved Hydrocarbon Contaminants
journal, August 2014
- Passeport, Elodie; Landis, Richard; Mundle, Scott O. C.
- Environmental Science & Technology, Vol. 48, Issue 16
Impact of Bioavailability Restrictions on Microbially Induced Stable Isotope Fractionation. 1. Theoretical Calculation
journal, July 2008
- Thullner, Martin; Kampara, Makeba; Richnow, Hans H.
- Environmental Science & Technology, Vol. 42, Issue 17
Chlorinated Solvents in Groundwater of the United States
journal, January 2007
- Moran, Michael J.; Zogorski, John S.; Squillace, Paul J.
- Environmental Science & Technology, Vol. 41, Issue 1
Chloroform respiration to dichloromethane by a Dehalobacter population: Growth-linked reductive dechlorination of chloroform
journal, January 2010
- Grostern, Ariel; Duhamel, Melanie; Dworatzek, Sandra
- Environmental Microbiology, Vol. 12, Issue 4
Complete Genome Sequence of Dehalobacterium formicoaceticum Strain DMC, a Strictly Anaerobic Dichloromethane-Degrading Bacterium
journal, September 2017
- Chen, Gao; Murdoch, Robert W.; Mack, E. Erin
- Genome Announcements, Vol. 5, Issue 37
Evaluation of Isotopic Enrichment Factors for the Biodegradation of Chlorinated Ethenes Using a Parameter Estimation Model: Toward an Improved Quantification of Biodegradation
journal, May 2006
- Morrill, Penny L.; Sleep, Brent E.; Slater, Gregory F.
- Environmental Science & Technology, Vol. 40, Issue 12
Natural chlorine isotope variations
journal, May 1984
- Kaufmann, Ronald; Long, Austin; Bentley, Harold
- Nature, Vol. 309, Issue 5966
The elusive roles of bacterial glutathione S- transferases: new lessons from genomes
journal, January 2002
- Pagni, S. Vuilleumier, M.
- Applied Microbiology and Biotechnology, Vol. 58, Issue 2
Monitoring Microbial Dechlorination of Tetrachloroethene (PCE) in Groundwater Using Compound-Specific Stable Carbon Isotope Ratios: Microcosm and Field Studies
journal, August 1999
- Hunkeler, D.; Aravena, R.; Butler, B. J.
- Environmental Science & Technology, Vol. 33, Issue 16
Insights into Enzyme Kinetics of Chloroethane Biodegradation Using Compound Specific Stable Isotopes
journal, October 2010
- Lollar, Barbara Sherwood; Hirschorn, Sarah; Mundle, Scott O. C.
- Environmental Science & Technology, Vol. 44, Issue 19
A D esulfitobacterium sp. strain PR reductively dechlorinates both 1,1,1-trichloroethane and chloroform : Strain PR dechlorinates TCA and chloroform
journal, February 2014
- Ding, Chang; Zhao, Siyan; He, Jianzhong
- Environmental Microbiology, Vol. 16, Issue 11
Stable carbon isotope fractionation during degradation of dichloromethane by methylotrophic bacteria
journal, January 2006
- Nikolausz, Marcell; Nijenhuis, Ivonne; Ziller, Katja
- Environmental Microbiology, Vol. 8, Issue 1
Impact of cell density on microbially induced stable isotope fractionation
journal, January 2009
- Kampara, Makeba; Thullner, Martin; Harms, Hauke
- Applied Microbiology and Biotechnology, Vol. 81, Issue 5
Analysis of Compound-Specific Chlorine Stable Isotopes of Vinyl Chloride by Continuous Flow–Isotope Ratio Mass Spectrometry (FC–IRMS)
journal, December 2009
- Shouakar-Stash, Orfan; Frape, S. K.; Aravena, R.
- Environmental Forensics, Vol. 10, Issue 4
Metabolism of Dichloromethane by the Strict Anaerobe Dehalobacterium formicoaceticum
journal, January 1998
- Mägli, Andreas; Messmer, Michael; Leisinger, Thomas
- Applied and Environmental Microbiology, Vol. 64, Issue 2
Volatile organic compounds in the nation's ground water and drinking-water supply wells
report, January 2006
- Zogorski, John S.; Carter, Janet M.; Ivahnenko, Tamara
- USGS
Dichloromethane dehalogenase of Hyphomicrobium sp. strain DM2.
journal, January 1985
- Kohler-Staub, D.; Leisinger, T.
- Journal of Bacteriology, Vol. 162, Issue 2
Bacterial dehalogenases: biochemistry, genetics, and biotechnological applications
journal, December 1994
- Fetzner, S.; Lingens, F.
- Microbiological Reviews, Vol. 58, Issue 4
Works referencing / citing this record:
Mineralization versus fermentation: evidence for two distinct anaerobic bacterial degradation pathways for dichloromethane
journal, January 2020
- Chen, Gao; Fisch, Alexander R.; Gibson, Caleb M.
- The ISME Journal, Vol. 14, Issue 4
Whole genome sequencing of a novel, dichloromethane-fermenting Peptococcaceae from an enrichment culture
journal, January 2019
- Holland, Sophie I.; Edwards, Richard J.; Ertan, Haluk
- PeerJ, Vol. 7
Multi-element (C, H, Cl, Br) stable isotope fractionation as a tool to investigate transformation processes for halogenated hydrocarbons
journal, January 2020
- Ojeda, Ann Sullivan; Phillips, Elizabeth; Sherwood Lollar, Barbara
- Environmental Science: Processes & Impacts, Vol. 22, Issue 3
Proteogenomics Reveals Novel Reductive Dehalogenases and Methyltransferases Expressed during Anaerobic Dichloromethane Metabolism
journal, January 2019
- Kleindienst, Sara; Chourey, Karuna; Chen, Gao
- Applied and Environmental Microbiology, Vol. 85, Issue 6
Dichloromethane and ethanol co-exposure aggravates oxidative stress indices causing hepatic and renal dysfunction in pubertal rats.
journal, January 2019
- Owumi, Solomon E.; Najophe, Eseroghene S.
- Toxicology Research and Application, Vol. 3