Agouti polynucleotide compositions and methods of use

Woychik, Richard P; Bultman, Scott J; Michaud, Edward J

Title: Agouti polynucleotide compositions and methods of use

Patent · Tue Feb 04 00:00:00 EST 2003

OSTI ID:875024

Woychik, Richard P ^[1]; Bultman, Scott J ^[2]; Michaud, Edward J ^[3]

Orinda, CA
Lakewood, OH
Kingston, TN

Disclosed are methods and compositions comprising novel agouti polypeptides and the polynucleotides which encode them. Also disclosed are DNA segments encoding these proteins derived from human and murine cell lines, and the use of these polynucleotides and polypeptides in a variety of diagnostic and therapeutic applications. Methods, compositions, kits, and devices are also provided for identifying compounds which are inhibitors of agouti activity, and for altering fatty acid synthetase activity and intracellular calcium levels in transformed cells.

View Patent

Cite

Export

Save

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Assignee:: UT-Battelle, LLC (Oak Ridge, TN)

Patent Number(s):: US 6514747

Application Number:: 09/781,811

OSTI ID:: 875024

Country of Publication:: United States

Language:: English

References (60)

Nutritional and endocrine modulation of intracellular calcium: Implications in obesity, insulin resistance and hypertension Zemel, Michael B.; Pierce, Grant N.; Izumi, Tohru Molecular and Cellular Effects of Nutrition on Disease Processes, p. 129-136 https://doi.org/10.1007/978-1-4615-5763-0_14	book	January 1998
An inherited limb deformity created by insertional mutagenesis in a transgenic mouse Woychik, R. P.; Stewart, T. A.; Davis, L. G. Nature, Vol. 318, Issue 6041 https://doi.org/10.1038/318036a0	journal	November 1985
Impairment of Glucose Tolerance in Yellow (A ^vy /A)(BALB/c × VY) F-l Hybrid Mice by Hyperglycemic Peptide(s) from Human Pituitary Glands* Frigeri, Luciano G.; Wolff, George L.; Robel, Greg Endocrinology, Vol. 113, Issue 6 https://doi.org/10.1210/endo-113-6-2097	journal	December 1983
A molecular model for the genetic and phenotypic characteristics of the mouse lethal yellow (Ay) mutation. Michaud, E. J.; Bultman, S. J.; Klebig, M. L. Proceedings of the National Academy of Sciences, Vol. 91, Issue 7 https://doi.org/10.1073/pnas.91.7.2562	journal	March 1994
Molecular structure and chromosomal mapping of the human homolog of the agouti gene. Kwon, H. Y.; Bultman, S. J.; Loffler, C. Proceedings of the National Academy of Sciences, Vol. 91, Issue 21 https://doi.org/10.1073/pnas.91.21.9760	journal	October 1994
Genomic Organization and Molecular Genetics of the Agouti Locus in the Mousea Siracusa, Linda D. Annals of the New York Academy of Sciences, Vol. 642, Issue 1 https://doi.org/10.1111/j.1749-6632.1991.tb24406.x	journal	December 1991
Transcriptional regulation of the adipocyte fatty acid synthase gene by agouti: interaction with insulin Claycombe, Kate J.; Wang, Yanxin; Jones, Brynn H. Physiological Genomics, Vol. 3, Issue 3 https://doi.org/10.1152/physiolgenomics.2000.3.3.157	journal	September 2000
Mechanisms for the pleiotropic effects of the agouti gene. Manne, J.; Argeson, A. C.; Siracusa, L. D. Proceedings of the National Academy of Sciences, Vol. 92, Issue 11 https://doi.org/10.1073/pnas.92.11.4721	journal	May 1995
Combined effects of insulin treatment and adipose tissue-specific agouti expression on the development of obesity Mynatt, R. L.; Miltenberger, R. J.; Klebig, M. L. Proceedings of the National Academy of Sciences, Vol. 94, Issue 3 https://doi.org/10.1073/pnas.94.3.919	journal	February 1997
Structure and function of ASP , the human homolog of the mouse agouti gene Wilson, Brent D.; Ollmann, Michael M.; Kang, Lana Human Molecular Genetics, Vol. 4, Issue 2 https://doi.org/10.1093/hmg/4.2.223	journal	January 1995
Molecular Biology: Obesity Sheds Its Secrets Gura, T. Science, Vol. 275, Issue 5301 https://doi.org/10.1126/science.275.5301.751	journal	February 1997
The embryonic lethality of homozygous lethal yellow mice (Ay/Ay) is associated with the disruption of a novel RNA-binding protein. Michaud, E. J.; Bultman, S. J.; Stubbs, L. J. Genes & Development, Vol. 7, Issue 7a https://doi.org/10.1101/gad.7.7a.1203	journal	July 1993
When does homology mean something else? Lewin, R. Science, Vol. 237, Issue 4822 https://doi.org/10.1126/science.3629257	journal	September 1987
Evidence of Increased Glyceraldehyde-3-phosphate Dehydrogenase and Fatty Acid Synthetase Promoter Activities in Transiently Transfected Adipocytes from Genetically Obese Rats Rolland, Violaine; Dugail, Isabelle; Liepvre, Xavier Le Journal of Biological Chemistry, Vol. 270, Issue 3 https://doi.org/10.1074/jbc.270.3.1102	journal	January 1995
Role of melanocortinergic neurons in feeding and the agouti obesity syndrome Fan, Wei; Boston, Bruce A.; Kesterson, Robert A. Nature, Vol. 385, Issue 6612 https://doi.org/10.1038/385165a0	journal	January 1997
Overexpression of anAgouticDNA in the Skin of Transgenic Mice Recapitulates Dominant Coat Color Phenotypes of Spontaneous Mutants Kucera, Gary T.; Bortner, Donna M.; Rosenberg, Michael P. Developmental Biology, Vol. 173, Issue 1 https://doi.org/10.1006/dbio.1996.0014	journal	January 1996
Mouse coat colour mutations: A molecular genetic resource which spans the centuries Jackson, Ian J. BioEssays, Vol. 13, Issue 9 https://doi.org/10.1002/bies.950130903	journal	September 1991
Studies on the high-sulphur proteins of reduced Merino wool. Amino acid sequence of protein SCMKB-IIIA3 Swart, Louis S.; Haylett, Thomas Biochemical Journal, Vol. 133, Issue 4 https://doi.org/10.1042/bj1330641	journal	August 1973
Molecular characterization of the mouse agouti locus Bultman, Scott J.; Michaud, Edward J.; Woychik, Richard P. Cell, Vol. 71, Issue 7 https://doi.org/10.1016/S0092-8674(05)80067-4	journal	December 1992
“Homology” in proteins and nucleic acids: A terminology muddle and a way out of it Reeck, Gerald R.; de Haën, Christoph; Teller, David C. Cell, Vol. 50, Issue 5 https://doi.org/10.1016/0092-8674(87)90322-9	journal	August 1987
Myosin light chain enhancer activates muscle-specific, developmentally regulated gene expression in transgenic mice. Rosenthal, N.; Kornhauser, J. M.; Donoghue, M. Proceedings of the National Academy of Sciences, Vol. 86, Issue 20 https://doi.org/10.1073/pnas.86.20.7780	journal	October 1989
Differential expression of a new dominant agouti allele (Aiapy) is correlated with methylation state and is influenced by parental lineage. Michaud, E. J.; van Vugt, M. J.; Bultman, S. J. Genes & Development, Vol. 8, Issue 12 https://doi.org/10.1101/gad.8.12.1463	journal	June 1994
Molecular and Developmental Genetics of Mouse Coat Color Jackson, I. J. Annual Review of Genetics, Vol. 28, Issue 1 https://doi.org/10.1146/annurev.ge.28.120194.001201	journal	December 1994
Targeted Disruption of the Melanocortin-4 Receptor Results in Obesity in Mice Huszar, Dennis; Lynch, Catherine A.; Fairchild-Huntress, Victoria Cell, Vol. 88, Issue 1 https://doi.org/10.1016/S0092-8674(00)81865-6	journal	January 1997
The role of insulin in the regulation of stearic acid desaturase activity in liver and adipose tissue from obese–hyperglycaemic (ob/ob) and lean mice Enser, M. Biochemical Journal, Vol. 180, Issue 3 https://doi.org/10.1042/bj1800551	journal	June 1979
Genomic organization and biosynthesis of secreted and cytoplasmic forms of gelsolin Kwiatkowski, Dj; Mehl, R.; Yin, Hl Journal of Cell Biology, Vol. 106, Issue 2 https://doi.org/10.1083/jcb.106.2.375	journal	February 1988
Craniofacial abnormalities induced by ectopic expression of the homeobox gene Hox-1.1 in transgenic mice Balling, Rudi; Mutter, George; Gruss, Peter Cell, Vol. 58, Issue 2 https://doi.org/10.1016/0092-8674(89)90848-9	journal	July 1989
The effects of calcium channel blockade on agouti ‐induced obesity Kim, Jung Han; Mynatt, Randall L.; Moore, Jerry W. The FASEB Journal, Vol. 10, Issue 14 https://doi.org/10.1096/fasebj.10.14.9002558	journal	December 1996
Agouti structure and function: characterization of a potent .alpha.-melanocyte stimulating hormone receptor antagonist Willard, Derril H.; Bodnar, Wanda; Harris, Cole Biochemistry, Vol. 34, Issue 38 https://doi.org/10.1021/bi00038a030	journal	September 1995
Phaeomelanin synthesis and obesity in mice Wolff, George L.; Galbraith, Donald B.; Domon, Olen E. Journal of Heredity, Vol. 69, Issue 5 https://doi.org/10.1093/oxfordjournals.jhered.a108953	journal	September 1978
Molecular and genetic characterization of a radiation-induced structural rearrangement in mouse chromosome 2 causing mutations at the limb deformity and agouti loci. Woychik, R. P.; Generoso, W. M.; Russell, L. B. Proceedings of the National Academy of Sciences, Vol. 87, Issue 7 https://doi.org/10.1073/pnas.87.7.2588	journal	April 1990
Agouti regulation of intracellular calcium: role of melanocortin receptors Kim, J. H.; Kiefer, L. L.; Woychik, R. P. American Journal of Physiology-Endocrinology and Metabolism, Vol. 272, Issue 3 https://doi.org/10.1152/ajpendo.1997.272.3.E379	journal	March 1997
Upregulation of adipocyte metabolism by agouti protein: possible paracrine actions in yellow mouse obesity Jones, B. H.; Kim, J. H.; Zemel, M. B. American Journal of Physiology-Endocrinology and Metabolism, Vol. 270, Issue 1 https://doi.org/10.1152/ajpendo.1996.270.1.E192	journal	January 1996
Increase in cytosolic free Ca2+ in corticotropin-stimulated white adipocytes Izawa, T.; Mochizuki, T.; Komabayashi, T. American Journal of Physiology-Endocrinology and Metabolism, Vol. 266, Issue 3 https://doi.org/10.1152/ajpendo.1994.266.3.E418	journal	March 1994
Adrenalectomy and response to corticosterone and MSH in the genetically obese yellow mouse Shimizu, H.; Shargill, N. S.; Bray, G. A. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, Vol. 256, Issue 2 https://doi.org/10.1152/ajpregu.1989.256.2.R494	journal	February 1989
Pancreatic Islet Cells in Preobese Yellow Avy/- Mice: Relation to Adult Hyperinsulinemia and Obesity Warbritton, A.; Gill, A. M.; Yen, T. T. Experimental Biology and Medicine, Vol. 206, Issue 2 https://doi.org/10.3181/00379727-206-43733	journal	June 1994
Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format. Kwoh, D. Y.; Davis, G. R.; Whitfield, K. M. Proceedings of the National Academy of Sciences, Vol. 86, Issue 4, p. 1173-1177 https://doi.org/10.1073/pnas.86.4.1173	journal	February 1989
Insulin increases fatty acid synthase gene transcription in human adipocytes Claycombe, Kate J.; Jones, Brynn H.; Standridge, Melissa K. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, Vol. 274, Issue 5 https://doi.org/10.1152/ajpregu.1998.274.5.R1253	journal	May 1998
Effects of ciglitazone on endogenous plasma islet amyloid polypeptide and insulin sensitivity in obese-diabetic viable yellow mice Gill, Anne M.; Yen, Terence T. Life Sciences, Vol. 48, Issue 7 https://doi.org/10.1016/0024-3205(91)90546-N	journal	January 1991
Positional cloning of the mouse obese gene and its human homologue Zhang, Yiying; Proenca, Ricardo; Maffei, Margherita Nature, Vol. 372, Issue 6505 https://doi.org/10.1038/372425a0	journal	December 1994
The long-range restriction map surrounding the mouse agouti locus reveals a disparity between physical and genetic distances Barsh, Gregory S.; Epstein, Charles J. Genomics, Vol. 5, Issue 1 https://doi.org/10.1016/0888-7543(89)90080-3	journal	July 1989
Mutations in the Carboxyl Terminus of the Agouti Protein Decrease Agouti Inhibition of Ligand Binding to the Melanocortin Receptors Kiefer, Laura L.; Ittoop, Olivia R. R.; Bunce, Keith Biochemistry, Vol. 36, Issue 8 https://doi.org/10.1021/bi962647v	journal	February 1997
Molecular characterization of a region of DNA associated with mutations at the agouti locus in the mouse. Bultman, S. J.; Russell, L. B.; Gutierrez-Espeleta, G. A. Proceedings of the National Academy of Sciences, Vol. 88, Issue 18 https://doi.org/10.1073/pnas.88.18.8062	journal	September 1991
The Role of the agouti Gene in the Yellow Obese Syndrome Miltenberger, Rosalynn J.; Mynatt, Randall L.; Wilkinson, J. Erby The Journal of Nutrition, Vol. 127, Issue 9 https://doi.org/10.1093/jn/127.9.1902S	journal	September 1997
Obesity-Associated Decrease in Growth Hormone-Releasing Hormone Gene Expression: A Mechanism for Reduced Growth Hormone mRNA Levels in Genetically Obese Zucker Rats Ahmad, Iqbal; Finkelstein, Judith A.; Downs, Thomas R. Neuroendocrinology, Vol. 58, Issue 3 https://doi.org/10.1159/000126558	journal	January 1993
Agouti protein is an antagonist of the melanocyte-stimulating-hormone receptor Lu, Dongsi; Willard, Derril; Patel, Indravadan R. Nature, Vol. 371, Issue 6500 https://doi.org/10.1038/371799a0	journal	October 1994
Role of the agouti gene in obesity Michaud, Ej; Mynatt, Rl; Miltenberger, Rj Journal of Endocrinology, Vol. 155, Issue 2 https://doi.org/10.1677/joe.0.1550207	journal	November 1997
Effects of a potent melanocortin agonist on the diabetic/obese phenotype in yellow mice Zemel, Mb; Moore, Jw; Moustaid, N. International Journal of Obesity, Vol. 22, Issue 7 https://doi.org/10.1038/sj.ijo.0800630	journal	July 1998
Cloning of the mouse agouti gene predicts a secreted protein ubiquitously expressed in mice carrying the lethal yellow mutation. Miller, M. W.; Duhl, D. M.; Vrieling, H. Genes & Development, Vol. 7, Issue 3 https://doi.org/10.1101/gad.7.3.454	journal	March 1993
Insulin increases vascular smooth muscle recovery from intracellular calcium loads. Kim, Y. C.; Zemel, M. B. Hypertension, Vol. 22, Issue 1 https://doi.org/10.1161/01.HYP.22.1.74	journal	July 1993
Obesity, diabetes, and neoplasia in yellow A ^vy /‐ mice: ectopic expression of the agouti gene Yen, Terence T.; Gill, Anne M.; Frigeri, Luciano G. The FASEB Journal, Vol. 8, Issue 8 https://doi.org/10.1096/fasebj.8.8.8181666	journal	May 1994
Agouti regulation of intracellular calcium: role in the insulin resistance of viable yellow mice. Zemel, M. B.; Kim, J. H.; Woychik, R. P. Proceedings of the National Academy of Sciences, Vol. 92, Issue 11 https://doi.org/10.1073/pnas.92.11.4733	journal	May 1995
The agouti gene product inhibits lipolysis in human adipocytes via a Ca ²⁺ ‐dependent mechanism Xue, Bingzhong; Moustaid‐Moussa, Naima; Wilkison, William O. The FASEB Journal, Vol. 12, Issue 13 https://doi.org/10.1096/fasebj.12.13.1391	journal	October 1998
Prenatal determination of obesity, tumor susceptibility, and coat color pattern in viable yellow (Avy/a) mice Wolff, G. L.; Roberts, D. W.; Galbraith, D. B. Journal of Heredity, Vol. 77, Issue 3 https://doi.org/10.1093/oxfordjournals.jhered.a110206	journal	May 1986
Conservation of the sequence of islet amyloid polypeptide in five mammals is consistent with its putative role as an islet hormone. Nishi, M.; Chan, S. J.; Nagamatsu, S. Proceedings of the National Academy of Sciences, Vol. 86, Issue 15 https://doi.org/10.1073/pnas.86.15.5738	journal	August 1989
Effects of Hypophysectomy and the Insulin-Like and Anti-Insulin Pituitary Peptides on Carbohydrate Metabolism in Yellow Avy/A (BALB/c x VY)F1 Hybrid Mice Salem, M. A. M.; Lewis, U. J.; Haro, L. S. Experimental Biology and Medicine, Vol. 191, Issue 4 https://doi.org/10.3181/00379727-191-42942	journal	September 1989
Ectopic expression of the agouti gene in transgenic mice causes obesity, features of type II diabetes, and yellow fur. Klebig, M. L.; Wilkinson, J. E.; Geisler, J. G. Proceedings of the National Academy of Sciences, Vol. 92, Issue 11 https://doi.org/10.1073/pnas.92.11.4728	journal	May 1995
Molecular Cloning, Expression, and Characterization of a Fifth Melanocortin Receptor Gantz, I.; Shimoto, Y.; Konda, Y. Biochemical and Biophysical Research Communications, Vol. 200, Issue 3 https://doi.org/10.1006/bbrc.1994.1580	journal	May 1994
Molecular analysis of reverse mutations from nonagouti (a) to black-and-tan (a(t)) and white-bellied agouti (Aw) reveals alternative forms of agouti transcripts. Bultman, S. J.; Klebig, M. L.; Michaud, E. J. Genes & Development, Vol. 8, Issue 4 https://doi.org/10.1101/gad.8.4.481	journal	February 1994
Impaired Recovery of Vascular Smooth Muscle Intracellular Calcium Following Agonist Stimulation in Insulin Resistant (Zucker Obese) Rats Zemel, Michael B.; Abel, Margaret A. American Journal of Hypertension, Vol. 6, Issue 6_Pt_1 https://doi.org/10.1093/ajh/6.6.500	journal	June 1993

Similar Records

Agouti polypeptide compositions

Patent · Tue Oct 30 00:00:00 EST 2001 · OSTI ID:875024

Woychik, Richard P; Bultman, Scott J; Michaud, Edward J

Restriction/modification polypeptides, polynucleotides, and methods

Patent · Tue Feb 24 00:00:00 EST 2015 · OSTI ID:875024

Westpheling, Janet; Chung, DaeHwan; Huddleston, Jennifer; +1 more

Agouti regulation of intracellular calcium: Role in the insulin resistance of viable yellow mice

Journal Article · Tue May 23 00:00:00 EDT 1995 · Proceedings of the National Academy of Sciences of the United States of America · OSTI ID:875024

Zemel, M B; Kim, J H; Woychik, R P; +4 more

Related Subjects

agouti
polynucleotide
compositions
methods
disclosed
comprising
novel
polypeptides
polynucleotides
encode
dna
segments
encoding
proteins
derived
human
murine
cell
lines
variety
diagnostic
therapeutic
applications
kits
devices
provided
identifying
compounds
inhibitors
activity
altering
fatty
acid
synthetase
intracellular
calcium
levels
transformed
cells
fatty acid
/435/999/

Title: Agouti polynucleotide compositions and methods of use

Citation Formats

References (60)

Similar Records

Related Subjects