Restriction/modification polypeptides, polynucleotides, and methods

Westpheling, Janet; Chung, DaeHwan; Huddleston, Jennifer; Farkas, Joel A

Restriction/modification polypeptides, polynucleotides, and methods

Patent · 24 February 2015

OSTI ID:1171089

Westpheling, Janet; Chung, DaeHwan; Huddleston, Jennifer; Farkas, Joel A

The present invention relates to the discovery of a novel restriction/modification system in Caldicellulosiruptor bescii. The discovered restriction enzyme is a HaeIII-like restriction enzyme that possesses a thermophilic activity profile. The restriction/modification system also includes a methyltransferase, M.CbeI, that methylates at least one cytosine residue in the CbeI recognition sequence to m.sup.4C. Thus, the invention provides, in various aspects, isolated CbeI or M.CbeI polypeptides, or biologically active fragments thereof; isolated polynucleotides that encode the CbeI or M.CbeI polypeptides or biologically active fragments thereof, including expression vectors that include such polynucleotide sequences; methods of digesting DNA using a CbeI polypeptide; methods of treating a DNA molecule using a M.CbeI polypeptide; and methods of transforming a Caldicellulosiruptor cell.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-00OR22725

Assignee:: University of Georgia Research Foundation, Inc. (Athens, GA)

Patent Number(s):: 8,962,333

Application Number:: 13/439,069

OSTI ID:: 1171089

Country of Publication:: United States

Language:: English

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