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Title: Cellulases and coding sequences

Patent ·
OSTI ID:873561

The present invention provides three fungal cellulases, their coding sequences, recombinant DNA molecules comprising the cellulase coding sequences, recombinant host cells and methods for producing same. The present cellulases are from Orpinomyces PC-2.

Research Organization:
Univ. of Georgia, Athens, GA (United States)
DOE Contract Number:
FG05-93ER20127
Assignee:
University of Georgia Research Foundation, Inc. (Athens, GA)
Patent Number(s):
US 6190189
Application Number:
09/286691
OSTI ID:
873561
Country of Publication:
United States
Language:
English

References (30)

Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis journal January 1988
A cyclophilin from the polycentric anaerobic rumen fungus Orpinomyces sp. strain PC-2 is highly homologous to vertebrate cyclophilin B. journal March 1995
Characterization of a Neocallimastix patriciarum cellulase cDNA (celA) homologous to Trichoderma reesei cellobiohydrolase II. journal January 1996
Supernatant protein and cellulase activities of the anaerobic ruminal fungus Neocallimastix frontalis EB188. journal January 1990
Endoglucanase CasA from alkalophilic Streptomyces strain KSM-9 is a typical member of family B of β-1,4-glucanases journal January 1993
Homologous catalytic domains in a rumen fungal xylanase: evidence for gene duplication and prokaryotic origin journal August 1992
A novel polysaccharide hydrolase cDNA (celD) from Neocallimastix patriciarum encoding three multi-functional catalytic domains with high endoglucanase, cellobiohydrolase and xylanase activities journal November 1992
Dissociation of the Cellulosome of Clostridium thermocellum in the Presence of Ethylenediaminetetraacetic Acid Occurs with the Formation of Truncated Polypeptides , journal January 1996
Cellulase families and their genes journal September 1987
Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase journal January 1994
Cloning, sequencing, and regulation of a xylanase gene from the fungus Aureobasidium pullulans Y-2311-1. journal January 1994
Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II journal January 1987
Molecular Biology of Cellulose Degradation journal October 1990
Crystal structure of the catalytic domain of a thermophilic endocellulase journal September 1993
Characterization and structure of an endoglucanase gene cenA of Cellulomonas fimi journal January 1986
Structural Role of Calcium for the Organization of the Cellulosome of Clostridium thermocellum journal January 1996
The Conserved Noncatalytic 40-Residue Sequence in Cellulases and Hemicellulases from Anaerobic Fungi Functions as a Protein Docking Domain journal December 1995
The nature of anaerobic fungi and their polysaccharide degrading enzymes journal July 1994
Essential 170-kDa subunit for degradation of crystalline cellulose by Clostridium cellulovorans cellulase. journal March 1990
Studies on the cellulase of the rumen anaerobic fungus Neocallimastix frontalis, with special reference to the capacity of the enzyme to degrade crystalline cellulose journal April 1992
The use of conserved cellulase family-specific sequences to clone cellulase homologue cDNAs from Fusarium oxysporum journal December 1994
Three-dimensional structure of cellobiohydrolase II from Trichoderma reesei journal July 1990
Cloning and expression of multiple cellulase cDNAs from the anaerobic rumen fungus Neocallimastix patriciarum in Escherichia coli journal July 1992
DNA sequences of three beta-1,4-endoglucanase genes from Thermomonospora fusca. journal January 1991
The Cellulosome: The Exocellular Organelle of Clostridium journal October 1993
Cellulase families revealed by hydrophobic cluster analysi journal September 1989
Isolation and properties of an extracellular beta-glucosidase from the polycentric rumen fungus Orpinomyces sp. strain PC-2. journal January 1994
The anchorage function of CipA (CelL), a scaffolding protein of the Clostridium thermocellum cellulosome. journal September 1995
Isolation, characterization, and analysis of the expression of the cbhII gene of Phanerochaete chrysosporium. journal January 1994
A highly active extracellular cellulase from the anaerobic rumen fungus Neocallimastix frontalis journal March 1986