Induction of mutagenesis and alterations in gene expression by tumorigenic chemicals
To determine the relationship between mutagenesis and carcinogenesis, a series of eleven polycyclic hydrocarbons with different degrees of carcinogenicity were tested in the cell-mediated mutagenesis assay for the induction of ouabain-resistant mutants. Four carcinogenic hydrocarbons induced ouabain-resistant mutants; five noncarcinogenic hydrocarbons were not mutagenic. Results indicated that there was a relationship between mutagenesis and the degree of carcinogenicity of polycyclic hydrocarbons after enhancement of their metabolism by aminophylline. To study liver carcinogens a system was developed for cocultivating primary liver cells and V79 hamster cells. In this system the nitrosamines and aflatoxins were metabolized by liver cells to intermediates that were mutagenic to the V79 cells. In experiments using human cells, tumor-promoting phorbol esters induced terminal differentiation while in other studies, in which avian and murine cells were employed, they inhibited differentiation. The results imply that human cells may respond differently from mouse and chicken cells to the biological effects of phorbol diesters. (HLW)
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6314920
- Report Number(s):
- CONF-790227-2
- Resource Relation:
- Conference: International workshop on short-term tests for prescreening of potential carcinogens, Genoa, Italy, 6 Feb 1979
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CARCINOGENESIS
MUTAGENESIS
CARCINOGENS
BIOLOGICAL EFFECTS
POLYCYCLIC AROMATIC HYDROCARBONS
CELL CULTURES
CHICKENS
ESTERS
HAMSTERS
LIVER
MAN
MICE
OUABAIN
ANIMALS
AROMATICS
BIRDS
BODY
CARBOHYDRATES
CARDIOTONICS
DIGESTIVE SYSTEM
DRUGS
FOWL
GLANDS
GLYCOSIDES
HYDROCARBONS
MAMMALS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PRIMATES
RODENTS
STEROIDS
STROPHANTIN
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)
550200 - Biochemistry