Induction of mutation and differentiation in mammalian cells by chemicals which initiate or promote tumor formation
A cell-mediated mutagenesis assay was developed to predict the potential carcinogenic hazard of some environmental chemicals. In this assay, cells with appropriate markers for mutagenesis, such as the Chinese hamster V79 cells, are co-cultivated with cells capable of metabolizing chemical carcinogens. Use of this assay made it possible to demonstrate a relationship between the degree of carcinogenicity and mutagenicity of a series of polycyclic hydrocarbons, nitrosamines and aflatoxins, and to establish means to study organ specificity of some chemical carcinogens. However, most short term in vitro assays are designed to detect mutagenic activity and therefore do not detect tumor promoting agents which are devoid of this activity. By analyzing various markers of terminal differentiation in cultured human melanoma and myeloid leukemia cells, we have established a relationship between the activity of a series of tumor promoting phorbol diesters in the mouse skin and their ability to induce terminal differentiation. We suggest that measuring alterations in the differentiation characteristics of some cultured cells may represent an approach by which environmental tumor promoting agents can be studied and detected.
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- Sponsoring Organization:
- USDOE Office of Health and Environmental Research
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 5338501
- Report Number(s):
- CONF-800742--1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Induction of mutagenesis and alterations in gene expression by tumorigenic chemicals
Control of cell transformation, mutagenesis, and differentiation by chemicals that initiate or promote tumor formation
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFLATOXIN
ANIMAL CELLS
ANIMALS
AROMATICS
BIOASSAY
CARCINOGENS
CELL CULTURES
CELL DIFFERENTIATION
HAMSTERS
HYDROCARBONS
MAMMALS
MUTAGEN SCREENING
NITROSO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
RODENTS
SCREENING
VERTEBRATES