Induction of mutagenesis and alterations in gene expression by tumorigenic chemicals
To determine the relationship between mutagenesis and carcinogenesis, a series of eleven polycyclic hydrocarbons with different degrees of carcinogenicity were tested in the cell-mediated mutagenesis assay for the induction of ouabain-resistant mutants. Four carcinogenic hydrocarbons induced ouabain-resistant mutants; five noncarcinogenic hydrocarbons were not mutagenic. Results indicated that there was a relationship between mutagenesis and the degree of carcinogenicity of polycyclic hydrocarbons after enhancement of their metabolism by aminophylline. To study liver carcinogens a system was developed for cocultivating primary liver cells and V79 hamster cells. In this system the nitrosamines and aflatoxins were metabolized by liver cells to intermediates that were mutagenic to the V79 cells. In experiments using human cells, tumor-promoting phorbol esters induced terminal differentiation while in other studies, in which avian and murine cells were employed, they inhibited differentiation. The results imply that human cells may respond differently from mouse and chicken cells to the biological effects of phorbol diesters. (HLW)
- Research Organization:
- Oak Ridge National Lab., TN (USA)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- W-7405-ENG-26
- OSTI ID:
- 6314920
- Report Number(s):
- CONF-790227--2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BIOLOGICAL EFFECTS
BIRDS
BODY
CARBOHYDRATES
CARCINOGENESIS
CARCINOGENS
CARDIOTONICS
CELL CULTURES
CHICKENS
DIGESTIVE SYSTEM
DRUGS
ESTERS
FOWL
GLANDS
GLYCOSIDES
HAMSTERS
HYDROCARBONS
LIVER
MAMMALS
MAN
MICE
MUTAGENESIS
ORGANIC COMPOUNDS
ORGANS
OUABAIN
PATHOGENESIS
POLYCYCLIC AROMATIC HYDROCARBONS
PRIMATES
RODENTS
STEROIDS
STROPHANTIN
VERTEBRATES