Capillary electrophoretic study of individual exocytotic events in single mast cells
- Iowa State Univ., Ames, IA (United States)
The peak profile of individual degranulation events from the on-column release of serotonin from single rat peritoneal mast cells (RPMCs) was monitored using capillary electrophoresis with laser-induced native fluorescence detection (CE-LINF). Serotonin, an important biogenic amine, is contained in granules (0.25 fL) within RPMCs and is extruded by a process termed exocytosis. The secretagogue, Polymyxin B sulfate, was used as the CE running buffer after injection of a single RPMC into the separation capillary to stimulate the release of the granules. Because the release process occurs on a ms time scale, monitoring individual exocytotic events is possible with the coupling of high-speed CE and LINF detection.
- Research Organization:
- Ames Lab., Ames, IA (United States)
- Sponsoring Organization:
- USDOE Office of Energy Research, Washington, DC (United States)
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 348903
- Report Number(s):
- IS-T-1860; ON: DE99002525; TRN: AHC29920%%53
- Resource Relation:
- Other Information: DN: Thesis submitted to Iowa State Univ., Ames, IA (US); TH: Thesis (M.S.); PBD: 12 Feb 1999
- Country of Publication:
- United States
- Language:
- English
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