Study of cell degranulation with simultaneous microscope imaging and capillary electrophoresis
Journal Article
·
· Applied Spectroscopy
- Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, Ames, Iowa 50011 (United States)
The physical release of single granules from individual rat peritoneal mast cells (RPMCs) was monitored by video recording of the degranulating cells by a high-resolution charge-coupled device (CCD) microscope system. The bright granular core disappears from the image as the vesicular content is dissolved on contact with the extra-cellular fluid. After a fixed time delay, the exocytotic product, serotonin, was detected by capillary electrophoresis coupled with laser-induced native fluorescence (LINF-CE). The timing of the two events is mostly correlated, which supports a fast release mechanism of the granular products. {copyright} {ital 1999} {ital Society for Applied Spectroscopy}
- Research Organization:
- Ames National Laboratory
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 686512
- Journal Information:
- Applied Spectroscopy, Vol. 53, Issue 7; Other Information: PBD: Jul 1999
- Country of Publication:
- United States
- Language:
- English
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