TNT cloning system

Tuskan, Gerald A.; Yang, Xiaohan; Cestari De Paoli, Henrique

Title: TNT cloning system

Patent · Tue Aug 15 00:00:00 EDT 2023

OSTI ID:2222169

Tuskan, Gerald A.; Yang, Xiaohan; Cestari De Paoli, Henrique

Disclosed herein are vectors and components for a nucleic acid cloning system, and methods of use of the vectors and components in cloning nucleic acid fragments of interest. The cloning system includes two families of destination vectors which can be used in alternating form to systematically combine nucleic acid fragments of interest.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-00OR22725

Assignee:: UT-Battelle, LLC (Oak Ridge, TN)

Patent Number(s):: 11,725,211

Application Number:: 17/138,369

OSTI ID:: 2222169

Resource Relation:: Patent File Date: 12/30/2020

Country of Publication:: United States

Language:: English

References (25)

Nucleocytoplasmic Distribution Is Required for Activation of Resistance by the Potato NB-LRR Receptor Rx1 and Is Balanced by Its Functional Domains Slootweg, Erik; Roosien, Jan; Spiridon, Laurentiu N. The Plant Cell, Vol. 22, Issue 12, p. 4195-4215 https://doi.org/10.1105/tpc.110.077537	journal	December 2010
REBASE—a database for DNA restriction and modification: enzymes, genes and genomes Roberts, Richard J.; Vincze, Tamas; Posfai, Janos Nucleic Acids Research, Vol. 43, Issue D1, p. D298-D299 https://doi.org/10.1093/nar/gku1046	journal	November 2014
The ‘cleavage’ activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring ‘2A-like’ sequences ten Dam, Edwin; Donnelly, Michelle L. L.; Gani, David Journal of General Virology, Vol. 82, Issue 5 https://doi.org/10.1099/0022-1317-82-5-1027	journal	May 2001
GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules Sarrion-Perdigones, Alejandro; Falconi, Erica Elvira; Zandalinas, Sara I. PLoS ONE, Vol. 6, Issue 7, Article No. e21622 https://doi.org/10.1371/journal.pone.0021622	journal	July 2011
GoldenBraid 2.0: A Comprehensive DNA Assembly Framework for Plant Synthetic Biology Sarrion-Perdigones, A.; Vazquez-Vilar, M.; Palaci, J. Plant Physiology, Vol. 162, Issue 3, p. 1618-1631 https://doi.org/10.1104/pp.113.217661	journal	May 2013
j5 DNA Assembly Design Automation Software Hillson, Nathan J.; Rosengarten, Rafael D.; Keasling, Jay D. ACS Synthetic Biology, Vol. 1, Issue 1, p. 14-21 https://doi.org/10.1021/sb2000116	journal	December 2011
Arabidopsis Tetraspanins Are Confined to Discrete Expression Domains and Cell Types in Reproductive Tissues and Form Homo- and Heterodimers When Expressed in Yeast Boavida, L. C.; Qin, P.; Broz, M. Plant Physiology, Vol. 163, Issue 2, p. 696-712 https://doi.org/10.1104/pp.113.216598	journal	August 2013
Method for the preparation of a nucleic acid library Van Den Brulle, Jan; Fuhrmann, Markus; Strohner, Ralf https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9115352 US Patent Document 9,115,352	patent	August 2015
Iterative plug-and-play methodology for constructing and modifying synthetic gene networks Litcofsky, Kevin D.; Afeyan, Raffi B.; Krom, Russell J. Nature Methods, Vol. 9, Issue 11, p. 1077-1080 https://doi.org/10.1038/nmeth.2205	journal	October 2012
Characterizing the Protonation State of Cytosine in Transient G·C Hoogsteen Base Pairs in Duplex DNA Nikolova, Evgenia N.; Goh, Garrett B.; Brooks, Charles L. Journal of the American Chemical Society, Vol. 135, Issue 18, p. 6766-6769 https://doi.org/10.1021/ja400994e	journal	April 2013
Intercalator conjugates of pyrimidine locked nucleic acid-modified triplex-forming oligonucleotides: improving DNA binding properties and reaching cellular activities Brunet, Erika; Corgnali, Maddalena; Perrouault, Loïc Nucleic Acids Research, Vol. 33, Issue 13, p. 4223-4234 https://doi.org/10.1093/nar/gki726	journal	July 2005
Enzymatic Assembly of Overlapping DNA Fragments Gibson, Daniel G. Methods in Enzymology https://doi.org/10.1016/B978-0-12-385120-8.00015-2	book	May 2011
A Modular Cloning System for Standardized Assembly of Multigene Constructs Weber, Ernst; Engler, Carola; Gruetzner, Ramona PLoS ONE, Vol. 6, Issue 2 https://doi.org/10.1371/journal.pone.0016765	journal	February 2011
Enzymatic assembly of DNA molecules up to several hundred kilobases Gibson, Daniel G.; Young, Lei; Chuang, Ray-Yuan Nature Methods, Vol. 6, Issue 5, p. 343-345 https://doi.org/10.1038/nmeth.1318	journal	April 2009
Synthetic biology as it relates to CAM photosynthesis: challenges and opportunities DePaoli, Henrique C.; Borland, Anne M.; Tuskan, Gerald A. Journal of Experimental Botany, Vol. 65, Issue 13, p. 3381-3393 https://doi.org/10.1093/jxb/eru038	journal	February 2014
A One Pot, One Step, Precision Cloning Method with High Throughput Capability Engler, Carola; Kandzia, Romy; Marillonnet, Sylvestre PLoS ONE, Vol. 3, Issue 11, Article No. e3647 https://doi.org/10.1371/journal.pone.0003647	journal	November 2008
Type IIS Restriction Enzyme Footprinting I. Measurement of a Triple Helix Dissociation Constant With Eco57I at 25°C Ward, B. Nucleic Acids Research, Vol. 24, Issue 12, p. 2435-2440 https://doi.org/10.1093/nar/24.12.2435	journal	June 1996
Protein Interaction Mapping in C. elegans Using Proteins Involved in Vulval Development Walhout, Albertha J. M.; Sordella, Raffaella; Lu, Xiaowei Science, Vol. 287, Issue 5450, p. 116-122 https://doi.org/10.1126/science.287.5450.116	journal	January 2000
Triple helix formation and the antigene strategy for sequence-specific control of gene expression Praseuth, D.; Guieysse, A. L.; Hélène, C. Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, Vol. 1489, Issue 1, p. 181-206 https://doi.org/10.1016/S0167-4781(99)00149-9	journal	December 1999
High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice Kim, Jin Hee; Lee, Sang-Rok; Li, Li-Hua PLoS ONE, Vol. 6, Issue 4, Article No. e18556 https://doi.org/10.1371/journal.pone.0018556	journal	April 2011
Transgenic Expression in Arabidopsis of a Polyprotein Construct Leading to Production of Two Different Antimicrobial Proteins François, Isabelle E. J. A.; De Bolle, Miguel F. C.; Dwyer, Geoff Plant Physiology, Vol. 128, Issue 4, p. 1346-1358 https://doi.org/10.1104/pp.010794	journal	April 2002
The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation Hajdukiewicz, Peter; Svab, Zora; Maliga, Pal Plant Molecular Biology, Vol. 25, Issue 6, p. 989-994 https://doi.org/10.1007/BF00014672	journal	September 1994
Endosomolytic polymers Rozem, David B.; Wakefield, Darren; Wolff, Jon A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040198687 US Patent Application 10/816081; 20040198687	patent-application	October 2004
Rapid, modular and reliable construction of complex mammalian gene circuits Guye, Patrick; Li, Yinqing; Wroblewska, Liliana Nucleic Acids Research, Vol. 41, Issue 16, p. e156-e156 https://doi.org/10.1093/nar/gkt605	journal	July 2013
Methods, compositions and kits for a one-step DNA cloning system Minshull, Jeremy; Ness, Jon E.; Theodorou, Elias https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9206433 US Patent Document 9,206,433	patent	December 2015

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