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Title: TNT cloning system

Patent ·
OSTI ID:1463001

Disclosed herein are vectors and components for a nucleic acid cloning system, and methods of use of the vectors and components in cloning nucleic acid fragments of interest. The cloning system includes two families of destination vectors which can be used in alternating form to systematically combine nucleic acid fragments of interest.

Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-000R22725
Assignee:
UT-BATTELLE, LLC (Oak Ridge, TN)
Patent Number(s):
10,017,770
Application Number:
14/789,112
OSTI ID:
1463001
Resource Relation:
Patent File Date: 2015 Jul 01
Country of Publication:
United States
Language:
English

References (23)

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Fast, easy and efficient: site-specific insertion of transgenes into Enterobacterial chromosomes using Tn7 without need for selection of the insertion event journal January 2006
GoldenBraid 2.0: A Comprehensive DNA Assembly Framework for Plant Synthetic Biology journal May 2013
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Arabidopsis Tetraspanins Are Confined to Discrete Expression Domains and Cell Types in Reproductive Tissues and Form Homo- and Heterodimers When Expressed in Yeast journal August 2013
Endosomolytic polymers patent-application October 2004
Iterative plug-and-play methodology for constructing and modifying synthetic gene networks journal October 2012
Characterizing the Protonation State of Cytosine in Transient G·C Hoogsteen Base Pairs in Duplex DNA
  • Nikolova, Evgenia N.; Goh, Garrett B.; Brooks, Charles L.
  • Journal of the American Chemical Society, Vol. 135, Issue 18, p. 6766-6769 https://doi.org/10.1021/ja400994e
journal April 2013
Intercalator conjugates of pyrimidine locked nucleic acid-modified triplex-forming oligonucleotides: improving DNA binding properties and reaching cellular activities journal July 2005
Methods, compositions and kits for a one-step DNA cloning system patent December 2015
Enzymatic assembly of DNA molecules up to several hundred kilobases journal April 2009
Synthetic biology as it relates to CAM photosynthesis: challenges and opportunities journal February 2014
A One Pot, One Step, Precision Cloning Method with High Throughput Capability journal November 2008
Type IIS Restriction Enzyme Footprinting I. Measurement of a Triple Helix Dissociation Constant With Eco57I at 25°C journal June 1996
Protein Interaction Mapping in C. elegans Using Proteins Involved in Vulval Development journal January 2000
High Cleavage Efficiency of a 2A Peptide Derived from Porcine Teschovirus-1 in Human Cell Lines, Zebrafish and Mice journal April 2011
Transgenic Expression in Arabidopsis of a Polyprotein Construct Leading to Production of Two Different Antimicrobial Proteins journal April 2002
The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation journal September 1994
Rapid, modular and reliable construction of complex mammalian gene circuits journal July 2013
Method for the preparation of a nucleic acid library patent August 2015

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