Analysis of the cyanobacterial amino acid metabolism with a precise genome-scale metabolic reconstruction of Anabaena sp. UTEX 2576

Norena-Caro, Daniel A.; Zuniga, Cristal; Pete, Amber J.; Saemundsson, Sven A.; Donaldson, Morgan R.; Adams, Alexandria J.; Dooley, Kerry M.; Zengler, Karsten; Benton, Michael G.

doi:10.1016/j.bej.2021.108008

Title: Analysis of the cyanobacterial amino acid metabolism with a precise genome-scale metabolic reconstruction of Anabaena sp. UTEX 2576

Journal Article · Wed Apr 07 00:00:00 EDT 2021 · Biochemical Engineering Journal

DOI:https://doi.org/10.1016/j.bej.2021.108008· OSTI ID:1851726

^[1]; Zuniga, Cristal ^[2]; Pete, Amber J. ^[1]; Saemundsson, Sven A. ^[1];

^[1]; Adams, Alexandria J. ^[1];

^[1]; Zengler, Karsten ^[3]; Benton, Michael G. ^[1]

Louisiana State University and A&M College, Baton Rouge, LA (United States)
University of California San Diego, La Jolla, CA (United States)
University of California San Diego, La Jolla, CA (United States); University of California San Diego, San Diego, CA (United States)

Filamentous cyanobacteria such as Anabaena sp. UTEX 2576 (a.k.a., Nostoc sp. PCC 7120) are a sustainable platform for commodity and specialty chemical production. Anabaena is a model cyanobacterium adopted to study production of nitrogen-containing metabolites useful for chemical, cosmetic, and pharmaceutical industries. Therefore, a precise description of the Anabaena metabolic network is desired to analyze their amino-acid metabolism and elucidate potential applications of these cyanobacteria as host organisms for biotechnological production. Secondary metabolite production depends on the metabolic availability of amino acids. We provide new insight on the biotechnological utilization of Anabaena after predicting phycocyanobilin and amino-acid production rates, using a genome-scale metabolic model (iDN1004). This metabolic reconstruction is a highly comprehensive representation of the global metabolism of Anabaena, which also contains experimental biomass equations and constraints under photoautotrophic and photodiazotrophic growth. Modelling results ranked proteinogenic amino acids based on predicted metabolic fluxes through amino-acid producing reactions. From these, l-aspartate, glycine, l-serine, l-valine, l-alanine, l-threonine, and l-leucine were selected as the best branching points to conduct metabolic engineering, where l-aspartate, l-serine, l-valine and glycine serve as potential precursors of the secondary metabolites Schizokinen (a siderophore), Sphingosine (a ceramide), Lyngbyatoxin A (an alkaloid), and Shinorine (a sunscreen).

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Research Organization:: Johns Hopkins University, Baltimore, MD (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0012658; SC0019388

OSTI ID:: 1851726

Alternate ID(s):: OSTI ID: 1777557

Journal Information:: Biochemical Engineering Journal, Vol. 171, Issue C; ISSN 1369-703X

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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The cyanobacterium Anabaena sp. PCC 7120 has two distinct β-carotene ketolases: CrtO for echinenone and CrtW for ketomyxol synthesis Mochimaru, Mari; Masukawa, Hajime; Takaichi, Shinichi FEBS Letters, Vol. 579, Issue 27 https://doi.org/10.1016/j.febslet.2005.09.081	journal	October 2005
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ABC-type neutral amino acid permease N-I is required for optimal diazotrophic growth and is repressed in the heterocysts of Anabaena sp. strain PCC 7120: Neutral amino acid permease in Anabaena Picossi, Silvia; Montesinos, María Luz; Pernil, Rafael Molecular Microbiology, Vol. 57, Issue 6 https://doi.org/10.1111/j.1365-2958.2005.04791.x	journal	July 2005
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The Azolla-Anabaena azollae Relationship: XII. Nitrogenase Activity and Phycobiliproteins of the Endophyte as a Function of Leaf Age and Cell Type Kaplan, Drora; Calvert, Harry E.; Peters, Gerald A. Plant Physiology, Vol. 80, Issue 4 https://doi.org/10.1104/pp.80.4.884	journal	April 1986
Engineering cyanobacteria to generate high-value products Ducat, Daniel C.; Way, Jeffrey C.; Silver, Pamela A. Trends in Biotechnology, Vol. 29, Issue 2, p. 95-103 https://doi.org/10.1016/j.tibtech.2010.12.003	journal	February 2011
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Genome-Scale Metabolic Model for the Green Alga Chlorella vulgaris UTEX 395 Accurately Predicts Phenotypes under Autotrophic, Heterotrophic, and Mixotrophic Growth Conditions Zuñiga, Cristal; Li, Chien-Ting; Huelsman, Tyler Plant Physiology, Vol. 172, Issue 1 https://doi.org/10.1104/pp.16.00593	journal	July 2016
Cloning, sequencing, and regulation of the global nitrogen regulator gene ntcA in the unicellular diazotrophic cyanobacterium Cyanothece sp. strain BH68K. Bradley, R. L.; Reddy, K. J. Journal of bacteriology, Vol. 179, Issue 13 https://doi.org/10.1128/JB.179.13.4407-4410.1997	journal	January 1997

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