Study of Stationary Phase Metabolism Via Isotopomer Analysis of Amino Acids from an Isolated Protein
Journal Article
·
· Biotechnology Progress
OSTI ID:973610
Microbial production of many commercially important secondary metabolites occurs during stationary phase, and methods to measure metabolic flux during this growth phase would be valuable. Metabolic flux analysis is often based on isotopomer information from proteinogenic amino acids. As such, flux analysis primarily reflects the metabolism pertinent to the growth phase during which most proteins are synthesized. To investigate central metabolism and amino acids synthesis activity during stationary phase, addition of fully 13C-labeled glucose followed by induction of green fluorescent protein (GFP) expression during stationary phase was used. Our results indicate that Escherichia coli was able to produce new proteins (i.e., GFP) in the stationary phase, and the amino acids in GFP were mostly from degraded proteins synthesized during the exponential growth phase. Among amino acid biosynthetic pathways, only those for serine, alanine, glutamate/glutamine, and aspartate/asparagine had significant activity during the stationary phase.
- Research Organization:
- Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
- Sponsoring Organization:
- Physical Biosciences Division
- DOE Contract Number:
- AC02-05CH11231
- OSTI ID:
- 973610
- Report Number(s):
- LBNL-2598E
- Journal Information:
- Biotechnology Progress, Journal Name: Biotechnology Progress; ISSN 8756-7938; ISSN BIPRET
- Country of Publication:
- United States
- Language:
- English
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