Free amino acid content and metabolic activities of setting and aborting soybean ovaries. [Glycine max (L. ) Merr]
Fruits of soybean (glycine max (L.) Merr.) that are destined to abscise shortly after anthesis grow more slowly than fruits that will be retained. In this work, amino acid composition, protein metabolism, and nucleic acid metabolism were studied in setting and abscising soybean ovaries from anthesis to 6 days after anthesis. Principal free amino acids were asparagine, aspartic acid, glutamic acid, serine, and glutamine. Percent aspartate and glutamate declined as the ovaries grew, with aspartate declining more in abscising and glutamate more in setting ovaries. Percent glutamate was positively correlated to percent abscission throughout the period. Proline, serine, and leucine were positively correlated to abscission from 0 to 2 days after anthesis, whereas significant negative correlations were observed at these ages for ethanolamine and arginine. /sup 75/Se fed as selenate and /sup 14/C fed as sucrose, glycine, and alanine were readily incorporated into soluble and insoluble proteins in a 24-hour in vitro incubation. Radioactivity of total proteins, expressed on a per-ovary basis, was negatively correlated with percent abscission and positively correlated with ovary weight. (/sup 14/C)Glutamine and serine followed the opposite pattern, with greater protein labeling in abscising than in setting ovaries. When data were expressed as disintegrations per minute per milligram ovary fresh weight, protein labeling from alanine was seen to be significantly greater in abscising ovaries at anthesis and throughout the sampling period. Nucleic acid labeling from uridine was highly correlated to ovary weight; labeling from thymidine was greater in setting than abscising ovaries at anthesis and in abscising ovaries at later stages of development.
- Research Organization:
- South Dakota State Univ., Brookings (USA)
- OSTI ID:
- 7164396
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 85:1; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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551001 -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALANINES
AMIDES
AMINES
AMINO ACIDS
ASPARAGINE
ASPARTIC ACID
AZINES
AZOLES
CARBOHYDRATES
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
DISACCHARIDES
EVEN-EVEN NUCLEI
FOOD
GLUTAMINE
GLYCINE
GROWTH
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY ACIDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUCINE
METABOLISM
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANT GROWTH
PLANTS
PROLINE
PROTEINS
PYRIMIDINES
PYRROLES
PYRROLIDINES
RIBOSIDES
SACCHARIDES
SACCHAROSE
SELENIUM 74
SELENIUM ISOTOPES
SERINE
SOYBEANS
STABLE ISOTOPES
THYMIDINE
TRACER TECHNIQUES
URACILS
URIDINE
VEGETABLES