Production of polyhydroxy alkanoates with a defined composition from an unrelated carbon source
Patent
·
OSTI ID:1823870
Cells and methods for producing polyhydroxyalkanoates. The cells comprise one or more recombinant genes selected from an R-specific enoyl-CoA hydratase gene, a PHA polymerase gene, a thioesterase gene, and an acyl-CoA-synthetase gene. The cells further have one or more genes functionally deleted. The functionally deleted genes include such genes as an enoyl-CoA hydratase gene, a 3-hydroxyacyl-CoA dehydrogenase, and a 3-ketoacyl-CoA thiolase gene. The recombinant cells are capable of using producing polyhydroxyalkanoates with a high proportion of monomers having the same carbon length from non-lipid substrates, such as carbohydrates.
- Research Organization:
- Univ. of Wisconsin, Madison, WI (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FC02-07ER64494
- Assignee:
- Wisconsin Alumni Research Foundation (Madison, WI)
- Patent Number(s):
- 10,982,240
- Application Number:
- 15/915,964
- OSTI ID:
- 1823870
- Resource Relation:
- Patent File Date: 03/08/2018
- Country of Publication:
- United States
- Language:
- English
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