Seedling Chloroplast Responses Induced by N-Linolenoylethanolamine Require Intact G-Protein Complexes

Journal Article · Tue Jul 14 00:00:00 EDT 2020 · Plant Physiology (Bethesda)

DOI:https://doi.org/10.1104/pp.19.01552· OSTI ID:1784478

Yan, Chengshi ^[1]; Cannon, Ashley E.  ^[1]; Watkins, Justin ^[2]; Keereetaweep, Jantana  ^[3]; Khan, Bibi Rafeiza ^[4]; Jones, Alan M.  ^[2]; Blancaflor, Elison B.  ^[4]; Azad, Rajeev K. ^[5]; Chapman, Kent D.  ^[3]

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1. Univ. of North Texas, Denton, TX (United States). Dept. of Biological Sciences and BioDiscovery Institute
2. Univ. of North Carolina, Chapel Hill, NC (United States). Depts. of Biology, and Pharmacology
3. Univ. of North Texas, Denton, TX (United States). Dept. of Biological Sciences and BioDiscovery Institute
4. Noble Research Institute, LLC, Ardmore, OK (United States)
5. Univ. of North Texas, Denton, TX (United States). Dept. of Biological Sciences and BioDiscovery Institute; Noble Research Institute, LLC, Ardmore, OK (United States); Univ. of North Texas, Denton, TX (United States). Dept. of Mathematics

In animals, several long-chain N-acylethanolamines (NAEs) have been identified as endocannabinoids and are autocrine signals that operate through cell surface G-protein-coupled cannabinoid receptors. Despite the occurrence of NAEs in land plants, including nonvascular plants, their precise signaling properties and molecular targets are not well defined. Here we show that the activity of N-linolenoylethanolamine (NAE 18:3) requires an intact G-protein complex. Specifically, genetic ablation of the Gbg dimer or loss of the full set of atypical Ga subunits strongly attenuates an NAE-18:3-induced degreening of cotyledons in Arabidopsis (Arabidopsis thaliana) seedlings. This effect involves, at least in part, transcriptional regulation of chlorophyll biosynthesis and catabolism genes. In addition, there is feedforward transcriptional control of G-protein signaling components and G-protein interactors. These results are consistent with NAE 18:3 being a lipid signaling molecule in plants with a requirement for G-proteins to mediate signal transduction, a situation similar, but not identical, to the action of NAE endocannabinoids in animal systems.

• Research Organization: Brookhaven National Lab. (BNL), Upton, NY (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF)
• Grant/Contract Number: SC0012704; IOS-1656263
• OSTI ID: 1784478
• Alternate ID(s): OSTI ID: 1811471
• Report Number(s): BNL-221399-2021-JAAM; BNL-221939-2021-JAAM
• Journal Information: Plant Physiology (Bethesda), Vol. 184, Issue 1; ISSN 0032-0889
• Publisher: American Society of Plant Biologists
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES