Overexpression of Fatty Acid Amide Hydrolase Induces Early Flowering in Arabidopsis thaliana

Teaster, Neal D.; Keereetaweep, Jantana; Kilaru, Aruna; Wang, Yuh-Shuh; Tang, Yuhong; Tran, Christopher N. -Q.; Ayre, Brian G.; Chapman, Kent D.; Blancaflor, Elison B.

doi:10.3389/fpls.2012.00032

Title: Overexpression of Fatty Acid Amide Hydrolase Induces Early Flowering in Arabidopsis thaliana

Journal Article · Sun Jan 01 00:00:00 EST 2012 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2012.00032· OSTI ID:1628222

Teaster, Neal D. ^[1]; Keereetaweep, Jantana ^[2]; Kilaru, Aruna ^[3]; Wang, Yuh-Shuh ^[4]; Tang, Yuhong ^[1]; Tran, Christopher N. -Q. ^[2]; Ayre, Brian G. ^[2]; Chapman, Kent D. ^[2]; Blancaflor, Elison B. ^[1]

The Samuel Roberts Noble Foundation, Ardmore, OK (United States)
Univ. of North Texas, Denton, TX (United States). Center for Plant Lipid Research. Dept. of Biological Sciences
East Tennessee State Univ., Johnson City, TN (United States). Dept. of Biological Sciences
Univ. of Tartu (Estonia). Inst. of Technology. Plant Signal Research Group

N-acylethanolamines (NAEs) are bioactive lipids derived from the hydrolysis of the membrane phospholipid N-acylphosphatidylethanolamine (NAPE). In animal systems this reaction is part of the “endocannabinoid” signaling pathway, which regulates a variety of physiological processes. The signaling function of NAE is terminated by fatty acid amide hydrolase (FAAH), which hydrolyzes NAE to ethanolamine and free fatty acid. Our previous work in Arabidopsis thaliana showed that overexpression of AtFAAH (At5g64440) lowered endogenous levels of NAEs in seeds, consistent with its role in NAE signal termination. Reduced NAE levels were accompanied by an accelerated growth phenotype, increased sensitivity to abscisic acid (ABA), enhanced susceptibility to bacterial pathogens, and early flowering. Here we investigated the nature of the early flowering phenotype of AtFAAH overexpression. AtFAAH over-expressors flowered several days earlier than wild type and AtFAAH knockouts under both non-inductive short day (SD) and inductive long day (LD) conditions. Microarray analysis revealed that the FLOWERING LOCUS T (FT ) gene, which plays a major role in regulating flowering time, and one target MADS box transcription factor, SEPATALLA3 (SEP3), were elevated in AtFAAH over-expressors. Furthermore, AtFAAH over-expressors, with the early flowering phenotype had lower endogenous NAE levels in leaves compared to wild type prior to flowering. Exogenous application of NAE 12:0, which was reduced by up to 30% in AtFAAH over-expressors, delayed the onset of flowering in wild type plants. We conclude that the early flowering phenotype of AtFAAH over-expressors is, in part, explained by elevated FT gene expression resulting from the enhanced NAE hydrolase activity of AtFAAH, suggesting that NAE metabolism may participate in floral signaling pathways.

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Research Organization:: Univ. of North Texas, Denton, TX (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: FG02-05ER15647

OSTI ID:: 1628222

Journal Information:: Frontiers in Plant Science, Vol. 3; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Plant Sciences
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Title: Overexpression of Fatty Acid Amide Hydrolase Induces Early Flowering in Arabidopsis thaliana

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