In vitro methods for processing lignin and other aromatic compounds

Donohue, Timothy James; Gall, Daniel Leo; Kontur, Wayne S.; Kim, Hoon Seok; Ralph, John; Noguera, Daniel R.; Fox, Brian G.; Bingman, Craig

Title: In vitro methods for processing lignin and other aromatic compounds

Patent · Tue Nov 10 00:00:00 EST 2020

OSTI ID:1771603

Donohue, Timothy James; Gall, Daniel Leo; Kontur, Wayne S.; Kim, Hoon Seok; Ralph, John; Noguera, Daniel R.; Fox, Brian G.; Bingman, Craig

Enzymes for depolymerizing lignin. The enzymes include dehydrogenases, β-etherases, and glutathione lyases. The dehydrogenases can comprise one or more or LigD, LigO, LigN, and LigL. The β-etherases can comprise one or more of LigE, LigF, LigP, and BaeA. The glutathione lyases can comprise any one or more of LigG and a number of non-stereospecific, optionally recombinant glutathione lyases derived from Sphingobium sp. SYK-6, Novosphingobium aromaticivorans, Escherichia coli, Streptococcus sanguinis, Phanerochaete chrysosporium, and other microorganisms. The enzymes can be combined in compositions and/or used in methods of processing lignin or other aromatic compounds in vitro.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FC02-07ER64494; SC0018409

Assignee:: Wisconsin Alumni Research Foundation (Madison, WI)

Patent Number(s):: 10,829,745

Application Number:: 16/103,275

OSTI ID:: 1771603

Resource Relation:: Patent File Date: 08/14/2018

Country of Publication:: United States

Language:: English

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