In vitro methods of chemical conversion using non-stereospecific glutathione lyases

Donohue, Timothy James; Gall, Daniel Leo; Kontur, Wayne S.; Kim, Hoon; Ralph, John; Noguera, Daniel R.; Fox, Brian; Bingman, Craig

In vitro methods of chemical conversion using non-stereospecific glutathione lyases

Patent · Tue Sep 20 00:00:00 EDT 2022

OSTI ID:1925119

Donohue, Timothy James; Gall, Daniel Leo; Kontur, Wayne S.; Kim, Hoon; Ralph, John; Noguera, Daniel R.; Fox, Brian; Bingman, Craig

Enzymes for depolymerizing lignin. The enzymes include dehydrogenases, β-etherases, and glutathione lyases. The dehydrogenases can comprise one or more or LigD, LigO, LigN, and LigL. The β-etherases can comprise one or more of LigE, LigF, LigP, and BaeA. The glutathione lyases can comprise any one or more of LigG and a number of non-stereospecific, optionally recombinant glutathione lyases derived from Sphingobium sp. SYK-6, Novosphingobium aromaticivorans, Escherichia coli, Streptococcus sanguinis, Phanerochaete chrysosporium, and other microorganisms. The enzymes can be combined in compositions and/or used in methods of processing lignin or other aromatic compounds in vitro.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FC02-07ER64494; SC0018409

Assignee:: Wisconsin Alumni Research Foundation (Madison, WI)

Patent Number(s):: 11,447,754

Application Number:: 17/038,971

OSTI ID:: 1925119

Country of Publication:: United States

Language:: English

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