Linker-Mediated Assembly of Virus-Like Particles into Ordered Arrays via Electrostatic Control

Brunk, Nicholas E.; Uchida, Masaki; Lee, Byeongdu; Fukuto, Masafumi; Yang, Lin; Douglas, Trevor; Jadhao, Vikram

doi:10.1021/acsabm.9b00166

Title: Linker-Mediated Assembly of Virus-Like Particles into Ordered Arrays via Electrostatic Control

Journal Article · Mon Apr 22 00:00:00 EDT 2019 · ACS Applied Bio Materials

DOI:https://doi.org/10.1021/acsabm.9b00166· OSTI ID:1716724

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^[2]; Fukuto, Masafumi ^[3]; Yang, Lin ^[3];

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Indiana Univ., Bloomington, IN (United States)
Argonne National Lab. (ANL), Lemont, IL (United States)
Brookhaven National Lab. (BNL), Upton, NY (United States)

Nanoscale virus-like particles (VLPs), which are self-assembled from protein subunits, offer the possibility of generating hierarchically assembled functional materials such as biomimetic catalytic systems and optical metamaterials. We explore the capacity to control and tune a higher-order assembly of VLPs into ordered array materials over a wide range of ionic conditions using a combination of experimental and computational methods. The integrated methodology demonstrates that P22 VLP variants, genetically engineered to exhibit different surface charges, self-assemble into ordered arrays in the presence of PAMAM dendrimers acting as oppositely charged, macromolecular linkers. Ordered assembly occurs at an optimal ionic strength that strongly correlates with the VLP surface charge. The ordered VLP arrays exhibit the same long-range order and a similar configuration of VLP-bound dendrimers, regardless of the VLP surface charge. The experimentally validated model identifies key electrostatic and kinetic mechanisms underlying the self-assembly process and guides the modulation of dendrimer concentration as a control parameter to tune the assembly of VLPs. Furthermore, the integrated approach opens new design and control strategies to fabricate active functional materials via the self-assembly of engineered VLPs.

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Research Organization:: Indiana Univ., Bloomington, IN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES). Materials Sciences & Engineering Division; National Institutes of Health (NIH)

Grant/Contract Number:: SC0016155; AC02-98CH10886; KP1605010; P41GM111244; S10 OD012331; AC02-06CH11357

OSTI ID:: 1716724

Journal Information:: ACS Applied Bio Materials, Vol. 2, Issue 5; ISSN 2576-6422

Publisher:: ACS PublicationsCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
self-assembly
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electrostatic control
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biomimetic materials
kinetic control
molecular dynamics simulations
ionic strength
lattices
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surface charge
dendrons

Title: Linker-Mediated Assembly of Virus-Like Particles into Ordered Arrays via Electrostatic Control

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