Templated Assembly of a Functional Ordered Protein Macromolecular Framework from P22 Virus-like Particles

McCoy, Kimberly; Uchida, Masaki; Lee, Byeongdu; Douglas, Trevor

doi:10.1021/acsnano.8b00528

Title: Templated Assembly of a Functional Ordered Protein Macromolecular Framework from P22 Virus-like Particles

Journal Article · Tue Mar 20 00:00:00 EDT 2018 · ACS Nano

DOI:https://doi.org/10.1021/acsnano.8b00528· OSTI ID:1461340

McCoy, Kimberly ^[1];

^[1];

^[2];

^[1]

Indiana Univ., Bloomington, IN (United States)
Argonne National Lab. (ANL), Argonne, IL (United States)

Bottom-up construction of mesoscale materials using biologically derived nanoscale building blocks enables engineering of desired physical properties using green production methods. Virus-like particles (VLPs) are exceptional building blocks due to their monodispersed sizes, geometric shapes, production ease, proteinaceous composition, and our ability to independently functionalize the interior and exterior interfaces. Here a VLP, derived from bacteriophage P22, is used as a building block for the fabrication of a protein macromolecular framework (PMF), a tightly linked 3D network of functional protein cages that exhibit long-range order and catalytic activity. Assembly of PMFs was electrostatically templated, using amine-terminated dendrimers, then locked into place with a ditopic cementing protein that binds to P22. Long-range order is preserved on removal of the dendrimer, leaving a framework material composed completely of protein. Furthermore, encapsulation of β-glucosidase enzymes inside of P22 VLPs results in formation of stable, condensed-phase materials with high local concentration of enzymes generating catalytically active PMFs.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1461340

Journal Information:: ACS Nano, Vol. 12, Issue 4; ISSN 1936-0851

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 39 works

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Title: Templated Assembly of a Functional Ordered Protein Macromolecular Framework from P22 Virus-like Particles

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