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Title: Genome analysis of Desulfotomaculum gibsoniae strain GrollT a highly versatile Gram-positive sulfate-reducing bacterium

Journal Article · · Standards in Genomic Sciences
DOI:https://doi.org/10.4056/sigs.5209235· OSTI ID:1628693
 [1];  [2];  [3];  [3];  [2];  [2];  [2];  [4];  [5];  [6];  [7];  [8];  [9];  [10];  [9];  [8];  [8];  [11];  [12];  [12] more »;  [13] « less
  1. Bremen Inst. for Materials Testing, Bremen (Germany). Dept. of Microbiology
  2. Wageningen Univ. (Netherlands). Lab. of Microbiology
  3. Albert-Ludwigs Univ., Freiburg (Germany). Inst. of Biology II
  4. Wageningen Univ. (Netherlands). Lab. of Systems and Synthetic Biology
  5. Univ. of Amsterdam (Netherlands). Inst. for Biodiversity and Ecosystem Dynamics. Dept. of Aquatic Microbiology
  6. Universidade Nova de Lisboa, Oeiras (Portugal). Instituto de Tecnologia Quimica e Biologica
  7. Russian Academy of Sciences (RAS), Moscow (Russian Federation). Winogradsky Inst. of Microbiology
  8. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Bioscience Division
  9. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  10. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Bioscience Division
  11. HZI-Helmholtz Centre for Infection Research, Braunschweig (Germany)
  12. Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig (Germany)
  13. Wageningen Univ. (Netherlands). Lab. of Microbiology; Univ. of Minho, Braga (Portugal). Centre of Biological Engineering
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Desulfotomaculum gibsoniae is a mesophilic member of the polyphyletic spore-forming genus Desulfotomaculum within the family Peptococcaceae. This bacterium was isolated from a freshwater ditch and is of interest because it can grow with a large variety of organic substrates, in particular several aromatic compounds, short-chain and mediumchain fatty acids, which are degraded completely to carbon dioxide coupled to the reduction of sulfate. It can grow autotrophically with H2 + CO2 and sulfate and slowly acetogenically with H2 + CO2, formate or methoxylated aromatic compounds in the absence of sulfate. It does not require any vitamins for growth. Here, we describe the features of D. gibsoniae strain GrollT together with the genome sequence and annotation. The chromosome has 4,855,529 bp organized in one circular contig and is the largest genome of all sequenced Desulfotomaculum spp. to date. A total of 4,666 candidate protein-encoding genes and 96 RNA genes were identified. Genes of the acetyl-CoA pathway, possibly involved in heterotrophic growth and in CO2 fixation during autotrophic growth, are present. The genome contains a large set of genes for the anaerobic transformation and degradation of aromatic compounds, which are lacking in the other sequenced Desulfotomaculum genomes.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1628693
Journal Information:
Standards in Genomic Sciences, Vol. 9, Issue 3; ISSN 1944-3277
Publisher:
BioMed CentralCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (9)

Temporal Responses of Microbial Communities to Anaerobic Soil Disinfestation journal December 2019
The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways journal January 2018
The ecology of anaerobic degraders of BTEX hydrocarbons in aquifers journal November 2016
Bioreactor microbial ecosystems with differentiated methanogenic phenol biodegradation and competitive metabolic pathways unraveled with genome-resolved metagenomics journal May 2018
A year of monitoring 20 mesophilic full-scale bioreactors reveals the existence of stable but different core microbiomes in bio-waste and wastewater anaerobic digestion systems journal July 2018
Metagenomic, phylogenetic, and functional characterization of predominant endolithic green sulfur bacteria in the coral Isopora palifera journal January 2019
Exemplar Abstract for Desulfallas gibsoniae (Kuever et al. 1999) Watanabe et al. 2018, Desulfotomaculum gibsoniae Kuever et al. 1999 and Desulfoscipio gibsoniae (Kuever et al. 1999) Watanabe et al. 2021. dataset January 2013
Comparative Proteomic Analysis of Desulfotomaculum reducens MI-1: Insights into the Metabolic Versatility of a Gram-Positive Sulfate- and Metal-Reducing Bacterium journal February 2016
Sulfur Respiration in a Group of Facultatively Anaerobic Natronoarchaea Ubiquitous in Hypersaline Soda Lakes journal October 2018

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Genetics & Heredity
Microbiology
spore-forming anaerobes
sulfate reduction
autotrophic
anaerobic degradation of aromatic compounds
complete oxidizer
Peptococcaceae
Clostridiales