The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans

Zhang, L.; Ward, J. D.; Cheng, Z.; Dernburg, A. F.

doi:10.1242/dev.129635

Title: The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans

Journal Article · Mon Nov 09 00:00:00 EST 2015 · Development (Cambridge)

DOI:https://doi.org/10.1242/dev.129635· OSTI ID:1627086

Zhang, L. ^[1]; Ward, J. D. ^[2]; Cheng, Z. ^[3]; Dernburg, A. F. ^[1]

Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology; Howard Hughes Medical Inst., Chevy Chase, MD (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Life Sciences Division. Dept. of Genome Dynamics; California Inst. for Quantitative Biosciences, Berkeley, CA (United States)
Univ. of California, San Francisco, CA (United States). Dept. of Cellular and Molecular Pharmacology
Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology

Experimental manipulation of protein abundance in living cells or organisms is an essential strategy for investigation of biological regulatory mechanisms. Whereas powerful techniques for protein expression have been developed in Caenorhabditis elegans, existing tools for conditional disruption of protein function are far more limited. To address this, we have adapted the auxin-inducible degradation (AID) system discovered in plants to enable conditional protein depletion in C. elegans. We report that expression of a modified Arabidopsis TIR1 F-box protein mediates robust auxin-dependent depletion of degron-tagged targets. We document the effectiveness of this system for depletion of nuclear and cytoplasmic proteins in diverse somatic and germline tissues throughout development. Target proteins were depleted in as little as 20-30 min, and their expression could be re-established upon auxin removal. We have engineered strains expressing TIR1 under the control of various promoter and 3' UTR sequences to drive tissue-specific or temporally regulated expression. The degron tag can be efficiently introduced by CRISPR/Cas9-based genome editing. We have harnessed this system to explore the roles of dynamically expressed nuclear hormone receptors in molting, and to analyze meiosis-specific roles for proteins required for germ line proliferation. Together, our results demonstrate that the AID system provides a powerful new tool for spatiotemporal regulation and analysis of protein function in a metazoan model organism.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1627086

Journal Information:: Development (Cambridge), Vol. 142, Issue 24; ISSN 0950-1991

Publisher:: Company of BiologistsCopyright Statement

Country of Publication:: United States

Language:: English

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Conditional control of fluorescent protein degradation by an auxin-dependent nanobody Daniel, Katrin; Icha, Jaroslav; Horenburg, Cindy Nature Communications https://doi.org/10.1101/298257	posted_content	May 2018
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A terminal selector prevents a Hox transcriptional switch to safeguard motor neuron identity throughout life Feng, Weidong; Li, Yinan; Dao, Pauline eLife, Vol. 9 https://doi.org/10.7554/eLife.50065	journal	January 2020
Auxin-Mediated Sterility Induction System for Longevity and Mating Studies in Caenorhabditis elegans Kasimatis, Katja R.; Moerdyk-Schauecker, Megan J.; PHillips, Patrick C. G3: Genes\|Genomes\|Genetics https://doi.org/10.1101/284232	posted_content	March 2018
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Microtubule assembly and pole coalescence: early steps in C. elegans oocyte meiosis I spindle assembly Chuang, Chien-Hui; Schlientz, Aleesa J.; Yang, Jie Biology Open https://doi.org/10.1242/bio.052308	journal	January 2020
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Caenorhabditis elegans PIEZO Channel Coordinates Multiple Reproductive Tissues to Govern Ovulation Bai, Xiaofei; Bouffard, Jeff; Lord, Avery TAGC 2020 https://doi.org/10.6084/m9.figshare.12133197	image	January 2020
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