Dynamic zinc fluxes regulate meiotic progression in Caenorhabditis elegans

Mendoza, Adelita D.; Sue, Aaron; Antipova, Olga; Vogt, Stefan; Woodruff, Teresa K.; Wignall, Sarah M.; O’Halloran, Thomas V.

doi:10.1093/biolre/ioac064

Dynamic zinc fluxes regulate meiotic progression in Caenorhabditis elegans

Journal Article · Sat Apr 23 00:00:00 EDT 2022 · Biology of Reproduction

DOI:https://doi.org/10.1093/biolre/ioac064· OSTI ID:1882017

Mendoza, Adelita D.; Sue, Aaron; Antipova, Olga; Vogt, Stefan; Woodruff, Teresa K.; Wignall, Sarah M.; O’Halloran, Thomas V.

Abstract

Zinc influx and efflux events are essential for meiotic progression in oocytes of several mammalian and amphibian species, but it is less clear whether this evolutionary conservation of zinc signals is also important in late-stage germline development in invertebrates. Using quantitative, single cell elemental mapping methods, we find that Caenorhabditis elegans oocytes undergo significant stage-dependent fluctuations in total zinc content, rising by over sevenfold from Prophase I through the beginning of mitotic divisions in the embryo. Live imaging of the rapid cell cycle progression in C. elegans enables us to follow changes in labile zinc pools across meiosis and mitosis in single embryo. We find a dynamic increase in labile zinc prior to fertilization that then decreases from Anaphase II through pronuclear fusion and relocalizes to the eggshell. Disruption of these zinc fluxes blocks extrusion of the second polar body, leading to a range of mitotic defects. We conclude that spatial temporal zinc fluxes are necessary for meiotic progression in C. elegans and are a conserved feature of germ cell development in a broad cross section of metazoa.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1882017

Alternate ID(s):: OSTI ID: 1864621
OSTI ID: 1988770

Journal Information:: Biology of Reproduction, Journal Name: Biology of Reproduction Journal Issue: 2 Vol. 107; ISSN 0006-3363

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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