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Title: Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Heterogeneity Zones

Journal Article · · Genome Biology and Evolution
DOI:https://doi.org/10.1093/gbe/evw135· OSTI ID:1625340
 [1];  [2];  [3];  [2];  [2];  [4];  [5];  [6];  [7];  [8];  [9];  [3];  [1]
  1. California State Univ. Fullerton, Fullerton, CA (United States). Center for Applied Biotechnology Studies, Dept. of Biological Science
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Bioscience Div.
  3. Univ.of Buenos Aires, Buenos Aires, Argentina. IMPaM (UBA-CONICET)
  4. Univ. of North Carolina, Chapel Hill, NC (United States). Div. of Infectious Diseases
  5. California State Univ. Fullerton, Fullerton, CA (United States). Center for Applied Biotechnology Studies, Dept. of Biological Science; Univ. of Oxford, United Kingdom. Dept. of Biochemistry
  6. Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH (United States)
  7. Case Western Reserve Univ. School of Medicine, Cleveland, OH (United States). Dept. of Medicine
  8. Univ. of Oxford, United Kingdom. Dept. of Biochemistry
  9. Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, OH (United States) Case Western Reserve Univ. School of Medicine, Cleveland, OH (United States). Dept. of Medicine
+ Show Author Affiliations

Klebsiella pneumoniae has become one of the most dangerous causative agents of hospital infections due to the acquisition of resistance to carbapenems, one of the last resort families of antibiotics. Resistance is usually mediated by carbapenemases coded for by different classes of genes. A prolonged outbreak of carbapenem-resistant K. pneumoniae infections has been recently described in northeastern Ohio. Most strains isolated from patients during this outbreak belong to MLST sequence type 258 (ST258). To understand more about this outbreak two isolates (strains 140 and 677), one of them responsible for a fatal infection, were selected for genome comparison analyses. Whole genome map and sequence comparisons demonstrated that both strains are highly related showing 99% average nucleotide identity. However, the genomes differ at the so-called high heterogeneity zone (HHZ) and other minor regions. This study identifies the potential value of the HHZ as a potential marker for K. pneumoniae clinical and epidemiological studies.

Research Organization:
Lawrence Berkeley National Lab (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC); National Institutes of Health (NIH), National Institute of Allergy and Infectious Diseases (NIAID) Public Health Service grant; Wellcome Trust Program Grant; Cleveland Department of Veterans Affairs, Veterans Affairs Merit Review Program; Geriatric Research Education and Clinical Center; LA Basin Minority Health and Health Disparities International Research Training Program (MHIRT)
Grant/Contract Number:
AC02-05CH11231; 2R15AI047115-04; R01AI063517; R01AI10056; WT083469; 1I01BX001974; 5T37MD001368
OSTI ID:
1625340
Journal Information:
Genome Biology and Evolution, Vol. 8, Issue 6; ISSN 1759-6653
Publisher:
Society for Molecular Biology and EvolutionCopyright Statement
Country of Publication:
United States
Language:
English

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