Recalcitrance Assessment of the Agro-industrial Residues from Five Agave Species: Ionic Liquid Pretreatment, Saccharification and Structural Characterization

Pérez-Pimienta, José A.; Mojica-Álvarez, Reyna M.; Sánchez-Herrera, Leticia M.; Mittal, Ashutosh; Sykes, Robert W.

doi:10.1007/s12155-018-9920-5

Title: Recalcitrance Assessment of the Agro-industrial Residues from Five Agave Species: Ionic Liquid Pretreatment, Saccharification and Structural Characterization

Journal Article · Tue May 15 00:00:00 EDT 2018 · BioEnergy Research

DOI:https://doi.org/10.1007/s12155-018-9920-5· OSTI ID:1474460

Pérez-Pimienta, José A. ^[1]; Mojica-Álvarez, Reyna M. ^[1]; Sánchez-Herrera, Leticia M. ^[2]; Mittal, Ashutosh ^[3]; Sykes, Robert W. ^[4]

Autonomous Univ. of Nayarit, Tepic (Mexico). Dept. of Chemical Engineering
Autonomous Univ. of Nayarit, Tepic (Mexico). Food Technology Unit
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center

Agave has recently shown its potential as a bioenergy feedstock with promising features such as higher biomass productivity than leading bioenergy feedstock while at the same time being drought-resistant with low water requirements and high sugar to ethanol conversion using ionic liquid (IL) pretreatment. Here, IL pretreatment was studied to develop the first direct side-by-side comparative recalcitrance assessment of the agro-industrial residues from five Agave species [Agave americana (AME), A. angustifolia (ANG), A. fourcroydes (FOU), A. salmiana (SAL), and A. tequilana (TEQ)] using compositional analysis, X-ray diffraction, and the lignin syringyl/guaiacyl subunit ratio (S/G) by pyrolysis molecular beam mass spectrometry (PyMBMS). Prominent calcium oxalate peaks were found only in unpretreated AME, SAL, and TEQ. The S/G ratios of all five unpretreated Agave species were between 1.27 and 1.57 while the IL-pretreated samples were from 1.39 to 1.72. The highest overall sugar production was obtained with IL-pretreated FOU with 492 mg glucose/g biomass and 157 mg xylose/g biomass at 120°C and 3 h using 1-ethyl-3-methylimidazolium acetate ([C₂C₁Im][OAc]). An estimated theoretical ethanol yield from the studied agro-industrial residues from the five Agave species was in the range of 1060 to 5800 L ethanol/ha/year. These comparison results demonstrate the potential of the Agave spp. as a suitable biofuel feedstock which can be employed within a biorefinery scheme.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Contributing Organization:: Novozymes A/S, Copenhagen (Denmark); Bioagaves de la Costa, Tepic (Mexico); Destilería Leyros, Tequila (Mexico); Mezcal Koch, Oaxaca (Mexico); Hacienda Aké, Tixkokob (Mexico)

Grant/Contract Number:: AC05-00OR22725; AC36-08-GO28308

OSTI ID:: 1474460

Report Number(s):: NREL/JA-2700-71742

Journal Information:: BioEnergy Research, Vol. 11, Issue 3; ISSN 1939-1234

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 16 works

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Title: Recalcitrance Assessment of the Agro-industrial Residues from Five Agave Species: Ionic Liquid Pretreatment, Saccharification and Structural Characterization

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