Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery

Flores-Gómez, Carlos A.; Escamilla Silva, Eleazar M.; Zhong, Cheng; Dale, Bruce E.; da Costa Sousa, Leonardo; Balan, Venkatesh

doi:10.1186/s13068-017-0995-6

Title: Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery

Journal Article · Tue Jan 16 00:00:00 EST 2018 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-017-0995-6· OSTI ID:1618714

; Escamilla Silva, Eleazar M.; Zhong, Cheng; Dale, Bruce E.; da Costa Sousa, Leonardo;

Agave-based alcoholic beverage companies generate thousands of tons of solid residues per year in Mexico. These agave residues might be used for biofuel production due to their abundance and favorable sustainability characteristics. In this work, agave leaf and bagasse residues from species Agave tequilana and Agave salmiana were subjected to pretreatment using the ammonia fiber expansion (AFEX) process. The pretreatment conditions were optimized using a response surface design methodology. We also identified commercial enzyme mixtures that maximize sugar yields for AFEX-pretreated agave bagasse and leaf matter, at ~ 6% glucan (w/w) loading enzymatic hydrolysis. Finally, the pretreated agave hydrolysates (at a total solids loading of ~ 20%) were used for ethanol fermentation using the glucose- and xylose-consuming strain Saccharomyces cerevisiae 424A (LNH-ST), to determine ethanol yields at industrially relevant conditions.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: FC02-07ER64494

OSTI ID:: 1618714

Alternate ID(s):: OSTI ID: 1504761

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 11 Journal Issue: 1; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 34 works

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09 BIOMASS FUELS
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MOESM1 of Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery Flores-Gómez, Carlos; Silva, Eleazar Escamilla; Zhong, Cheng https://doi.org/10.6084/m9.figshare.5793318 The current record is supplemented by this text	text	January 2018
Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery Flores-Gómez, Carlos; Silva, Eleazar Escamilla; Zhong, Cheng https://doi.org/10.6084/m9.figshare.c.3978105.v1 The current record is supplemented by this collection	collection	January 2018
MOESM1 of Conversion of lignocellulosic agave residues into liquid biofuels using an AFEX™-based biorefinery Flores-Gómez, Carlos; Silva, Eleazar Escamilla; Zhong, Cheng https://doi.org/10.6084/m9.figshare.5793318.v1 The current record is supplemented by this text	text	January 2018