A comparative study of ethanol production using dilute acid, ionic liquid and AFEX™ pretreated corn stover

Uppugundla, Nirmal; da Costa Sousa, Leonardo; Chundawat, Shishir P. S.; Yu, Xiurong; Simmons, Blake; Singh, Seema; Gao, Xiadi; Kumar, Rajeev; Wyman, Charles E.; Dale, Bruce E.; Balan, Venkatesh

doi:10.1186/1754-6834-7-72

Title: A comparative study of ethanol production using dilute acid, ionic liquid and AFEX™ pretreated corn stover

Journal Article · Tue May 13 00:00:00 EDT 2014 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/1754-6834-7-72· OSTI ID:1511397

Uppugundla, Nirmal ^[1]; da Costa Sousa, Leonardo ^[1]; Chundawat, Shishir P. S. ^[2]; Yu, Xiurong ^[3]; Simmons, Blake ^[4]; Singh, Seema ^[4]; Gao, Xiadi ^[5]; Kumar, Rajeev ^[6]; Wyman, Charles E. ^[5]; Dale, Bruce E. ^[1]; Balan, Venkatesh ^[1]

Michigan State Univ., East Lansing, MI (United States). Dept. of Chemical Engineering and Materials Science. Dept. of Energy (DOE) Great Lakes Bioenergy Research Center (GLBRC)
Michigan State Univ., East Lansing, MI (United States). Dept. of Chemical Engineering and Materials Science. Dept. of Energy (DOE) Great Lakes Bioenergy Research Center (GLBRC); Univ. of Wisconsin, Madison, WI (United States). Dept. of Biochemistry. Dept. of Energy (DOE) Great Lakes Bioenergy Research Center (GLBRC)
Jilin TuoPai Agriculture Products Development Ltd, Jilin (China)
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States). Deconstruction Division; Sandia National Lab. (SNL-CA), Livermore, CA (United States). Biological and Material Science Center
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Univ. of California, Riverside, CA (United States). Dept. of Chemical and Environmental Engineering. Bourns College of Engineering. Center for Environmental Research and Technology (CE-CERT)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Univ. of California, Riverside, CA (United States). Center for Environmental Research and Technology (CE-CERT). Bourns College of Engineering

In a biorefinery producing cellulosic biofuels, biomass pretreatment will significantly influence the efficacy of enzymatic hydrolysis and microbial fermentation. Comparison of different biomass pretreatment techniques by studying the impact of pretreatment on downstream operations at industrially relevant conditions and performing comprehensive mass balances will help focus attention on necessary process improvements, and thereby help reduce the cost of biofuel production. An on-going collaboration between the three US Department of Energy (DOE) funded bioenergy research centers (Great Lakes Bioenergy Research Center (GLBRC), Joint BioEnergy Institute (JBEI) and BioEnergy Science Center (BESC)) has given us a unique opportunity to compare the performance of three pretreatment processes, notably dilute acid (DA), ionic liquid (IL) and ammonia fiber expansion (AFEX^TM), using the same source of corn stover. Separate hydrolysis and fermentation (SHF) was carried out using various combinations of commercially available enzymes and engineered yeast (Saccharomyces cerevisiae 424A) strain. The optimal commercial enzyme combination (Ctec2: Htec2: Multifect Pectinase, percentage total protein loading basis) was evaluated for each pretreatment with a microplate-based assay using milled pretreated solids at 0.2% glucan loading and 15 mg total protein loading/g of glucan. The best enzyme combinations were 67:33:0 for DA, 39:33:28 for IL and 67:17:17 for AFEX. The amounts of sugar (kg) (glucose: xylose: total gluco- and xylo-oligomers) per 100 kg of untreated corn stover produced after 72 hours of 6% glucan loading enzymatic hydrolysis were: DA (25:2:2), IL (31:15:2) and AFEX (26:13:7). Additionally, the amounts of ethanol (kg) produced per 100 kg of untreated corn stover and the respective ethanol metabolic yield (%) achieved with exogenous nutrient supplemented fermentations were: DA (14.0, 92.0%), IL (21.2, 93.0%) and AFEX (20.5, 95.0%), respectively. The reason for lower ethanol yield for DA is because most of the xylose produced during the pretreatment was removed and not converted to ethanol during fermentation. Compositional analysis of the pretreated biomass solids showed no significant change in composition for AFEX treated corn stover, while about 85% of hemicellulose was solubilized after DA pretreatment, and about 90% of lignin was removed after IL pretreatment. As expected, the optimal commercial enzyme combination was different for the solids prepared by different pretreatment technologies. Due to loss of nutrients during the pretreatment and washing steps, DA and IL pretreated hydrolysates required exogenous nutrient supplementation to ferment glucose and xylose efficiently, while AFEX pretreated hydrolysate did not require nutrient supplementation.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; FC02-07ER64494

OSTI ID:: 1511397

Journal Information:: Biotechnology for Biofuels, Vol. 7; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 163 works

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References (42)

Lignin monomer composition affects Arabidopsis cell-wall degradability after liquid hot water pretreatment Li, Xu; Ximenes, Eduardo; Kim, Youngmi Biotechnology for Biofuels, Vol. 3, Issue 1 https://doi.org/10.1186/1754-6834-3-27	journal	January 2010
Ethanolic fermentation of hydrolysates from ammonia fiber expansion (AFEX) treated corn stover and distillers grain without detoxification and external nutrient supplementation Lau, Ming W.; Dale, Bruce E.; Balan, Venkatesh Biotechnology and Bioengineering, Vol. 99, Issue 3 https://doi.org/10.1002/bit.21609	journal	January 2007
Lignocellulosic Biomass Pretreatment Using AFEX Balan, Venkatesh; Bals, Bryan; Chundawat, Shishir P. S. Biofuels, p. 61-77 https://doi.org/10.1007/978-1-60761-214-8_5	book	January 2009
Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment Chundawat, Shishir P. S.; Donohoe, Bryon S.; da Costa Sousa, Leonardo Energy & Environmental Science, Vol. 4, Issue 3, 973 https://doi.org/10.1039/c0ee00574f	journal	January 2011
Influence of physico-chemical changes on enzymatic digestibility of ionic liquid and AFEX pretreated corn stover Li, Chenlin; Cheng, Gang; Balan, Venkatesh Bioresource Technology, Vol. 102, Issue 13 https://doi.org/10.1016/j.biortech.2011.04.005	journal	July 2011
'Cradle-to-grave' assessment of existing lignocellulose pretreatment technologies Sousa, Leonardo; Chundawat, Shishir P. S.; Balan, Venkatesh Rutgers University https://doi.org/10.7282/t3cv4m03	text	January 2009
Challenges for recycling ionic liquids by using pressure driven membrane processes Haerens, Kurt; Van Deuren, Stephanie; Matthijs, Edward Green Chemistry, Vol. 12, Issue 12 https://doi.org/10.1039/c0gc00406e	journal	January 2010
Growth of the plant cell wall Cosgrove, Daniel J. Nature Reviews Molecular Cell Biology, Vol. 6, Issue 11, p. 850-861 https://doi.org/10.1038/nrm1746	journal	November 2005
Detoxification of a lignocellulosic biomass slurry by soluble polyelectrolyte adsorption for improved fermentation efficiency Carter, Brian; Squillace, Phillip; Gilcrease, Patrick C. Biotechnology and Bioengineering, Vol. 108, Issue 9 https://doi.org/10.1002/bit.23152	journal	April 2011
Comparison of dilute acid and ionic liquid pretreatment of switchgrass: Biomass recalcitrance, delignification and enzymatic saccharification Li, Chenlin; Knierim, Bernhard; Manisseri, Chithra Bioresource Technology, Vol. 101, Issue 13 https://doi.org/10.1016/j.biortech.2009.10.066	journal	July 2010
Recent process improvements for the ammonia fiber expansion (AFEX) process and resulting reductions in minimum ethanol selling price Sendich, Elizabeth (Newton); Laser, Mark; Kim, Seungdo Bioresource Technology, Vol. 99, Issue 17, p. 8429-8435 https://doi.org/10.1016/j.biortech.2008.02.059	journal	November 2008
Grassoline at the Pump Huber, George W.; Dale, Bruce E. Scientific American, Vol. 301, Issue 1 https://doi.org/10.1038/scientificamerican0709-52	journal	July 2009
Shear Deactivation of Cellulase, Exoglucanase, Endoglucanase, and β-Glucosidase in a Mechanically Agitated Reactor Gunjikar, T. P.; Sawant, S. B.; Joshi, J. B. Biotechnology Progress, Vol. 17, Issue 6 https://doi.org/10.1021/bp010114u	journal	December 2001
Multi-scale visualization and characterization of lignocellulosic plant cell wall deconstruction during thermochemical pretreatment Chundawat, Shishir P. S.; Donohoe, Bryon S.; Sousa, Leonardo Rutgers University https://doi.org/10.7282/t3j1062s	text	January 2011
Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations Berlin, Alex; Balakshin, Mikhail; Gilkes, Neil Journal of Biotechnology, Vol. 125, Issue 2, p. 198-209 https://doi.org/10.1016/j.jbiotec.2006.02.021	journal	September 2006
Comparative material balances around pretreatment technologies for the conversion of switchgrass to soluble sugars Garlock, Rebecca J.; Balan, Venkatesh; Dale, Bruce E. Bioresource Technology, Vol. 102, Issue 24 https://doi.org/10.1016/j.biortech.2011.04.002	journal	December 2011
Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals Chundawat, Shishir P. S.; Beckham, Gregg T.; Himmel, Michael E. Annual Review of Chemical and Biomolecular Engineering, Vol. 2, Issue 1, p. 121-145 https://doi.org/10.1146/annurev-chembioeng-061010-114205	journal	July 2011
Ionic liquid-mediated selective extraction of lignin from wood leading to enhanced enzymatic cellulose hydrolysis Lee, Sang Hyun; Doherty, Thomas V.; Linhardt, Robert J. Biotechnology and Bioengineering, Vol. 102, Issue 5, p. 1368-1376 https://doi.org/10.1002/bit.22179	journal	April 2009
Inhibition of cellulases by phenols Ximenes, Eduardo; Kim, Youngmi; Mosier, Nathan Enzyme and Microbial Technology, Vol. 46, Issue 3-4, p. 170-176 https://doi.org/10.1016/j.enzmictec.2009.11.001	journal	March 2010
Mixture optimization of six core glycosyl hydrolases for maximizing saccharification of ammonia fiber expansion (AFEX) pretreated corn stover Gao, Dahai; Chundawat, Shishir P. S.; Krishnan, Chandraraj Bioresource Technology, Vol. 101, Issue 8, p. 2770-2781 https://doi.org/10.1016/j.biortech.2009.10.056	journal	April 2010
DNA integrity and ecophysiological responses of Spanish populations of Ulmus glabra to increasing ozone levels Dell’Orso, Ambra; Kuzminsky, Elena; Bermejo-Bermejo, Victoria Ecotoxicology https://doi.org/10.1007/s10646-021-02436-z	journal	June 2021
Alkaline peroxide pretreatment of corn stover: effects of biomass, peroxide, and enzyme loading and composition on yields of glucose and xylose Banerjee, Goutami; Car, Suzana; Scott-Craig, John S. Biotechnology for Biofuels, Vol. 4, Issue 1 https://doi.org/10.1186/1754-6834-4-16	journal	January 2011
Supplementation with xylanase and β-xylosidase to reduce xylo-oligomer and xylan inhibition of enzymatic hydrolysis of cellulose and pretreated corn stover Qing, Qing; Wyman, Charles E. Biotechnology for Biofuels, Vol. 4, Issue 1, Article No: 18 https://doi.org/10.1186/1754-6834-4-18	journal	June 2011
Trends and challenges in the microbial production of lignocellulosic bioalcohol fuels Weber, Christian; Farwick, Alexander; Benisch, Feline Applied Microbiology and Biotechnology, Vol. 87, Issue 4 https://doi.org/10.1007/s00253-010-2707-z	journal	June 2010
Summary of findings from the Biomass Refining Consortium for Applied Fundamentals and Innovation (CAFI): corn stover pretreatment Elander, Richard T.; Dale, Bruce E.; Holtzapple, Mark Cellulose, Vol. 16, Issue 4 https://doi.org/10.1007/s10570-009-9308-y	journal	June 2009
Transition of Cellulose Crystalline Structure and Surface Morphology of Biomass as a Function of Ionic Liquid Pretreatment and Its Relation to Enzymatic Hydrolysis Cheng, Gang; Varanasi, Patanjali; Li, Chenlin Biomacromolecules, Vol. 12, Issue 4 https://doi.org/10.1021/bm101240z	journal	April 2011
Understanding the impact of ionic liquid pretreatment on eucalyptus Çetinkol, Özgül Persil; Dibble, Dean C.; Cheng, Gang Biofuels, Vol. 1, Issue 1 https://doi.org/10.4155/bfs.09.5	journal	January 2010
The impacts of pretreatment on the fermentability of pretreated lignocellulosic biomass: a comparative evaluation between ammonia fiber expansion and dilute acid pretreatment Lau, Ming W.; Gunawan, Christa; Dale, Bruce E. Biotechnology for Biofuels, Vol. 2, Issue 1 https://doi.org/10.1186/1754-6834-2-30	journal	January 2009
Process and technoeconomic analysis of leading pretreatment technologies for lignocellulosic ethanol production using switchgrass Tao, Ling; Aden, Andy; Elander, Richard T. Bioresource Technology, Vol. 102, Issue 24, p. 11105-11114 https://doi.org/10.1016/j.biortech.2011.07.051	journal	December 2011
Inhibition of enzymatic cellulolysis by phenolic compounds Tejirian, Ani; Xu, Feng Enzyme and Microbial Technology, Vol. 48, Issue 3 https://doi.org/10.1016/j.enzmictec.2010.11.004	journal	March 2011
Cellulosic ethanol production from AFEX-treated corn stover using Saccharomyces cerevisiae 424A(LNH-ST) Lau, M. W.; Dale, B. E. Proceedings of the National Academy of Sciences, Vol. 106, Issue 5, p. 1368-1373 https://doi.org/10.1073/pnas.0812364106	journal	January 2009
Comparative sugar recovery and fermentation data following pretreatment of poplar wood by leading technologies Wyman, Charles E.; Dale, Bruce E.; Elander, Richard T. Biotechnology Progress, Vol. 25, Issue 2 https://doi.org/10.1002/btpr.142	journal	March 2009
‘Cradle-to-grave’ assessment of existing lignocellulose pretreatment technologies da Costa Sousa, Leonardo; Chundawat, Shishir PS; Balan, Venkatesh Current Opinion in Biotechnology, Vol. 20, Issue 3 https://doi.org/10.1016/j.copbio.2009.05.003	journal	June 2009
Physical and chemical characterizations of corn stover and poplar solids resulting from leading pretreatment technologies Kumar, Rajeev; Mago, Gaurav; Balan, Venkatesh Bioresource Technology, Vol. 100, Issue 17 https://doi.org/10.1016/j.biortech.2009.01.075	journal	September 2009
A facile method for the recovery of ionic liquid and lignin from biomass pretreatment Dibble, Dean C.; Li, Chenlin; Sun, Lan Green Chemistry, Vol. 13, Issue 11 https://doi.org/10.1039/c1gc15111h	journal	January 2011
Fermentation of pretreated sugarcane bagasse hemicellulose hydrolysate to ethanol by Pachysolen tannophilus Cheng, Ke-Ke; Ge, Jing-Ping; Zhang, Jian-An Biotechnology Letters, Vol. 29, Issue 7 https://doi.org/10.1007/s10529-007-9361-2	journal	May 2007
Restructuring the Crystalline Cellulose Hydrogen Bond Network Enhances Its Depolymerization Rate Chundawat, Shishir P. S.; Bellesia, Giovanni; Uppugundla, Nirmal Journal of the American Chemical Society, Vol. 133, Issue 29 https://doi.org/10.1021/ja2011115	journal	July 2011
Production of Ethanol from Cellulosic Biomass Hydrolysates Using Genetically Engineered <I>Saccharomyces</I> Yeast Capable of Cofermenting Glucose and Xylose Sedlak, Miroslav; Ho, Nancy W. Y. Applied Biochemistry and Biotechnology, Vol. 114, Issue 1-3 https://doi.org/10.1385/abab:114:1-3:403	journal	January 2004
Influence of physico-chemical changes on enzymatic digestibility of ionic liquid and AFEX pretreated corn stover Li, Chenlin; Cheng, Gang; Balan, Venkatesh Rutgers University https://doi.org/10.7282/t30p128s	text	January 2011
Multifaceted characterization of cell wall decomposition products formed during ammonia fiber expansion (AFEX) and dilute acid based pretreatments Chundawat, Shishir P. S.; Vismeh, Ramin; Sharma, Lekh N. Bioresource Technology, Vol. 101, Issue 21 https://doi.org/10.1016/j.biortech.2010.06.027	journal	November 2010
The generation of fermentation inhibitors during dilute acid hydrolysis of softwood Larsson, Simona; Palmqvist, Eva; Hahn-Hägerdal, Bärbel Enzyme and Microbial Technology, Vol. 24, Issue 3-4 https://doi.org/10.1016/S0141-0229(98)00101-X	journal	February 1999
Inhibition Effects of Dilute-Acid Prehydrolysate of Corn Stover on Enzymatic Hydrolysis of Solka Floc Kothari, Urvi D.; Lee, Yoon Y. Applied Biochemistry and Biotechnology, Vol. 165, Issue 5-6 https://doi.org/10.1007/s12010-011-9355-3	journal	September 2011

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09 BIOMASS FUELS
AFEX
dilute acid
ionic liquid
pretreatment
enzymatic hydrolysis
cellulosic ethanol