Influence of Physico-Chemical Changes on Enzymatic Digestibility of Ionic Liquid and AFEX pretreated Corn Stover

Journal Article · Sat Jan 01 04:00:00 EST 2011 · Journal of the American Chemical Society

DOI:https://doi.org/10.1016/j.biortech.2011.04.005· OSTI ID:1015020

Li, Chenlin ^[1]; Cheng, Gang ^[1]; Kent, Michael S ^[2]; Ong, Markus ^[3]; Balan, Venkatesh ^[4]; Dale, Bruce E. ^[4]; Melnichenko, Yuri B ^[2]; Simmons, Blake ^[3]

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1. Joint Bioenergy Institute
2. ORNL
3. Sandia National Laboratories (SNL)
4. Michigan State University, East Lansing

Ionic liquid (IL) and ammonia fiber expansion (AFEX) pretreatments were studied to develop the first direct side-by-side comparative assessment on their respective impacts on biomass structure, composition, process mass balance, and enzymatic saccharification efficiency. AFEX pretreatment completely preserves plant carbohydrates, whereas IL pretreatment extracts 76% of hemicellulose. In contrast to AFEX, the native crystal structure of the recovered corn stover from IL pretreatment was significantly disrupted. For both techniques, more than 70% of the theoretical sugar yield was attained after 48 h of hydrolysis using commercial enzyme cocktails. IL pretreatment requires less enzyme loading and a shorter hydrolysis time to reach 90% yields. Hemicellulase addition led to significant improvements in the yields of glucose and xylose for AFEX pretreated corn stover, but not for IL pretreated stover. These results provide new insights into the mechanisms of IL and AFEX pretreatment, as well as the advantages and disadvantages of each.

• Research Organization: Oak Ridge National Laboratory (ORNL); High Flux Isotope Reactor
• Sponsoring Organization: SC USDOE - Office of Science (SC)
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1015020
• Journal Information: Journal of the American Chemical Society, Journal Name: Journal of the American Chemical Society Journal Issue: 13 Vol. 102; ISSN JACSAT; ISSN 0002-7863
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
AGRICULTURAL WASTES
AMMONIA
BIOMASS
CARBOHYDRATES
CRYSTAL STRUCTURE
EFFICIENCY
ENZYMES
FIBERS
GLUCOSE
HEMICELLULOSE
HYDROLYSIS
MAIZE
MASS BALANCE
MOLTEN SALTS
SACCHARIFICATION
SACCHAROSE
XYLOSE