Antibacterial dental adhesive resins containing nitrogen-doped titanium dioxide nanoparticles

Journal Article · Sat Sep 01 00:00:00 EDT 2018 · Materials Science and Engineering. C, Biomimetic Materials, Sensors and Systems

DOI:https://doi.org/10.1016/j.msec.2018.08.060· OSTI ID:1470843

Esteban Florez, Fernando Luis  ^[1]; Hiers, Rochelle Denise ^[1]; Larson, Preston ^[2]; Johnson, Matthew ^[2]; O'Rear, Edgar ^[2]; Rondinone, Adam J. ^[3]; Khajotia, Sharukh Soli ^[1]

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1. Univ. of Oklahoma Health Sciences Center, Oklahoma City, OK (United States)
2. Univ. of Oklahoma, Norman, OK (United States)
3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

The development of dental adhesive resins with long-lasting antibacterial properties is a possible solution to overcome the problem of secondary caries in modern adhesive dentistry.Objectives: (i) Synthesis and characterization of nitrogen-doped titanium dioxide nanoparticles (N_TiO₂), (ii) topographical, compositional and wettability characterization of thin-films (unaltered and experimental) and, (iii) antibacterial efficacy of N_TiO₂-containing dental adhesives against Streptococcus mutans biofilms.Materials and methods: Nanoparticles were synthesized and characterized using different techniques. Specimens (diameter = 12 mm, thickness ≅ 15 μm) of OptiBond Solo Plus (Kerr Corp., USA) and experimental adhesives [50, 67 and 80% (v/v)] were fabricated, photopolymerized (1000 mW/cm², 1 min) and UV-sterilized (254 nm, 800,000 μJ/cm²) for microscopy, spectroscopy, wettability and antibacterial testing. Wettability was assessed with a contact angle goniometer by dispensing water droplets (2 μL) onto four random locations of each specimen (16 drops/group). Drop profiles were recorded (1 min, 25 frames/s, 37 °C) and contact angles were calculated at time = 0 s (θ_INITIAL) and time = 59 s (θ_FINAL). Antibacterial testing was performed by growing S. mutans (UA159-ldh, JM10) biofilms for either 3 or 24 h (anaerobic conditions, 37 °C) with or without continuous light irradiation (410 ± 10 nm, 3 h = 38.75 J/cm², 24 h = 310.07 J/cm²) against the surfaces of sterile specimens.Results: N_TiO₂ was successfully prepared using solvothermal methods. Doped-nanoparticles displayed higher light absorption levels when compared to undoped titania. Experimental adhesives demonstrated superior antibacterial efficacy in dark conditions.Conclusions: The findings presented herein suggest that N_TiO₂ is a feasible antibacterial agent against cariogenic biofilms.

• Research Organization: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Basic Energy Sciences (BES)
• Grant/Contract Number: AC05-00OR22725
• OSTI ID: 1470843
• Journal Information: Materials Science and Engineering. C, Biomimetic Materials, Sensors and Systems, Vol. 93, Issue C; ISSN 0928-4931
• Publisher: Elsevier
• Country of Publication: United States
• Language: English

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36 MATERIALS SCIENCE