Sorption, solubility and cytotoxicity of novel antibacterial nanofilled dental adhesive resins

Esteban Florez, Fernando Luis; Kraemer, Hannah; Hiers, Rochelle Denise; Sacramento, Catharina Marques; Rondinone, Adam Justin; Silvério, Karina Gonzales; Khajotia, Sharukh S.

doi:10.1038/s41598-020-70487-z

Sorption, solubility and cytotoxicity of novel antibacterial nanofilled dental adhesive resins

Journal Article · Tue Aug 11 00:00:00 EDT 2020 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-020-70487-z· OSTI ID:1816599

Esteban Florez, Fernando Luis ^[1]; Kraemer, Hannah ^[2]; Hiers, Rochelle Denise ^[2]; Sacramento, Catharina Marques ^[3]; Rondinone, Adam Justin ^[4]; Silvério, Karina Gonzales ^[3]; Khajotia, Sharukh S. ^[2]

Univ. of Oklahoma, Oklahoma City, OK (United States). Health Sciences Center. College of Dentistry. Dept. of Restorative Sciences. Division of Dental Biomaterials; OSTI
Univ. of Oklahoma, Oklahoma City, OK (United States). Health Sciences Center. College of Dentistry. Dept. of Restorative Sciences. Division of Dental Biomaterials
State Univ. of Campinas, São Paulo (Brazil). Piracicaba Dental School. Dept. of Physiological Sciences. Dept. of Prosthodontics and Periodontics
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences (CNMS)

Dental adhesives hydrolyze in the mouth. This study investigated the water sorption (SOR), solubility (SOL) and cytotoxicity (CYTO) of experimental adhesives containing nitrogen-doped titanium dioxide nanoparticles (N_TiO₂). Specimens (n = 15/group [SOR, SOL]; n = 10/group [CYTO]) of unaltered Clearfil SE Protect (CSP), OptiBond Solo Plus (OSP), Adper Scotchbond (ASB) and experimental adhesives (OSP + 25% or 30% of N_TiO₂) were fabricated, desiccated (37 °C) and tested for SOR and SOL according to ISO Specification 4049 (2009). CYTO specimens were UV-sterilized (8 J/cm²) and monomer extracted in growth medium (1, 3 or 7 days). Human pulp cells were isolated and seeded (0.5 × 10⁴) for MTT assay. SOR and SOL data was analyzed using GLM and SNK (α = 0.05) and CYTO data was analyzed with Kruskal–Wallis and SNK tests (α = 0.05). SOR and SOL values ranged from 25.80 μg/mm³ (30% N_TiO₂) to 28.01 μg/mm³ (OSP) and 23.88 μg/mm³(30% N_TiO₂) to 25.39 μg/mm³ (25% N_TiO₂). CYTO results indicated that pulp cells exposed to experimental materials displayed comparable viabilities (p > 0.05) to those of OSP. Experimental materials displayed comparable SOR, SOL and CYTO values (p > 0.05) when compared to unaltered materials. N_TiO₂ incorporation have not adversely impacted SOR, SOL and CYTO properties of unaltered adhesives.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1816599

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 10; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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