An Overview of P450 Enzymes: Opportunity and Challenges in Industrial Applications
- National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
Cytochrome P450 enzymes (P450s) containing a heme-iron center, are biocatalysts from all kingdoms, involvedin a large variety of reactions. Their potential in catalyzing a broad range of substrates makes perfect candidates for biotechnology applications and the production of high-value compounds. Biocatalytic reactions performed by P450s have a great interest in the pharmaceutical industry, fine chemicals, cosmetics, and for bioremediation procedures. However, the complex nature of this protein is still a major hurdle in the prospect of using their promising ability for expanding the number of industrial applications. Multiple approaches of protein engineering are currently conducted to improve activity, stability and/or substrate specificity for a given reaction. Furthermore, in combination with the appropriate biocatalyst, a suitable bioengineering process is a key step in the implementation of P450s at the industrial scale.
- Research Organization:
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Organization:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- Grant/Contract Number:
- AC36-08GO28308
- OSTI ID:
- 1456863
- Report Number(s):
- NREL/JA-5100-71788
- Journal Information:
- Enzyme Engineering, Vol. 05, Issue 03; ISSN 2329-6674
- Publisher:
- OMICS InternationalCopyright Statement
- Country of Publication:
- United States
- Language:
- English
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