Cellulolytic and Xylanolytic Potential of High β-Glucosidase-Producing Trichoderma from Decaying Biomass

Okeke, Benedict C.

doi:10.1007/s12010-014-1121-x

Title: Cellulolytic and Xylanolytic Potential of High β-Glucosidase-Producing Trichoderma from Decaying Biomass

Journal Article · Sun Aug 17 00:00:00 EDT 2014 · Applied Biochemistry and Biotechnology

DOI:https://doi.org/10.1007/s12010-014-1121-x· OSTI ID:1419492

Okeke, Benedict C. ^[1]

Auburn Univ., Montgomery, AL (United States)

Availability, cost and efficiency of microbial enzymes for lignocellulose bioconversion are central to sustainable biomass ethanol technology. Fungi enriched from decaying biomass and surface soil mixture displayed an array of strong cellulolytic and xylanolytic activities. Strains SG2 and SG4 produced a promising array of cellulolytic and xylanolytic enzymes including β-glucosidase, usually low in cultures of Trichoderma species. Nucleotide sequence analysis of internal transcribed spacer 2 (ITS2) region of rRNA gene revealed that strains SG2 and SG4 are closely related to Trichoderma inhamatum, Trichoderma piluliferum and Trichoderma aureoviride. Trichoderma sp. SG2 correspondingly displayed as much as 9.84±1.12, 48.02±2.53 and 30.10±1.11 units mL-1 of cellulase, xylanase and β-glucosidase. Ten times dilution of culture supernatant of strain SG2 revealed that activities were about 5.34, 8.45, and 2.05 orders of magnitude higher than observed in crude culture filtrate for cellulase, xylanase, and β-glucosidase respectively, indicating that more enzymes are present to contact with substrates in biomass sacharification. In parallel experiments Trichoderma species SG2 and SG4 produced more β-glucosidase than the industrial strain Trichoderma reesei RUT-C30. Results indicate that strains SG2 and SG4 have potential for low cost in-house production of primary lignocellulose-hydrolyzing enzymes for production of biomass saccharides and biofuel in the field.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Auburn Univ., Montgomery, AL (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: EE0003132

OSTI ID:: 1419492

Journal Information:: Applied Biochemistry and Biotechnology, Vol. 174, Issue 4; ISSN 0273-2289

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 9 works

Citation information provided by
Web of Science

References (55)

Transglycosylation products of cellulase system ofTrichoderma reesei Vaheri, Marja; Leisola, M.; Kauppinen, V. Biotechnology Letters, Vol. 1, Issue 1 https://doi.org/10.1007/BF01395789	journal	January 1979
Simultaneous saccharification and fermentation of lignocellulosic residues pretreated with phosphoric acid–acetone for bioethanol production Li, Hui; Kim, Nag-Jong; Jiang, Min Bioresource Technology, Vol. 100, Issue 13 https://doi.org/10.1016/j.biortech.2009.01.021	journal	July 2009
Cellulases and biofuels Wilson, David B. Current Opinion in Biotechnology, Vol. 20, Issue 3, p. 295-299 https://doi.org/10.1016/j.copbio.2009.05.007	journal	June 2009
Xylanases, xylanase families and extremophilic xylanases Collins, Tony; Gerday, Charles; Feller, Georges FEMS Microbiology Reviews, Vol. 29, Issue 1, p. 3-23 https://doi.org/10.1016/j.femsre.2004.06.005	journal	January 2005
Lignocellulose conversion to biofuels: current challenges, global perspectives Himmel, Michael E.; Bayer, Edward A. Current Opinion in Biotechnology, Vol. 20, Issue 3 https://doi.org/10.1016/j.copbio.2009.05.005	journal	June 2009
‘Cradle-to-grave’ assessment of existing lignocellulose pretreatment technologies da Costa Sousa, Leonardo; Chundawat, Shishir PS; Balan, Venkatesh Current Opinion in Biotechnology, Vol. 20, Issue 3 https://doi.org/10.1016/j.copbio.2009.05.003	journal	June 2009
Enhanced enzyme production from mixed cultures of Trichoderma reesei RUT-C30 and Aspergillus niger LMA grown as fed batch in a stirred tank bioreactor Ahamed, Aftab; Vermette, Patrick Biochemical Engineering Journal, Vol. 42, Issue 1 https://doi.org/10.1016/j.bej.2008.05.007	journal	October 2008
Bacterial and plant cellulose modification using ultrasound irradiation Wong, Shen-Siung; Kasapis, Stefan; Tan, Yanfang Mabelyn Carbohydrate Polymers, Vol. 77, Issue 2 https://doi.org/10.1016/j.carbpol.2008.12.038	journal	June 2009
Biorefinery: Toward an industrial metabolism Octave, Stéphane; Thomas, Daniel Biochimie, Vol. 91, Issue 6 https://doi.org/10.1016/j.biochi.2009.03.015	journal	June 2009
Paper mill sludge as a potential source for cellulase production by Trichoderma reesei QM 9123 and Aspergillus niger using mixed cultivation Maheshwari, D. K.; Gohade, S.; Paul, J. Carbohydrate Polymers, Vol. 23, Issue 3 https://doi.org/10.1016/0144-8617(94)90098-1	journal	January 1994
Production of cellulase/β-glucosidase by the mixed fungi culture Trichoderma reesei and Aspergillus phoenicis on dairy manure Wen, Zhiyou; Liao, Wei; Chen, Shulin Process Biochemistry, Vol. 40, Issue 9 https://doi.org/10.1016/j.procbio.2005.03.044	journal	September 2005
The potential of cellulases and cellulosomes for cellulosic waste management Bayer, Edward A.; Lamed, Raphael; Himmel, Michael E. Current Opinion in Biotechnology, Vol. 18, Issue 3 https://doi.org/10.1016/j.copbio.2007.04.004	journal	June 2007
Characterization of a Defined Cellulolytic and Xylanolytic Bacterial Consortium for Bioprocessing of Cellulose and Hemicelluloses Okeke, Benedict C.; Lu, Jue Applied Biochemistry and Biotechnology, Vol. 163, Issue 7, p. 869-881 https://doi.org/10.1007/s12010-010-9091-0	journal	September 2010
Screening for cellulose and hemicellulose degrading enzymes from the fungal genus Ulocladium Pedersen, Mads; Hollensted, Morten; Lange, Lene International Biodeterioration & Biodegradation, Vol. 63, Issue 4 https://doi.org/10.1016/j.ibiod.2009.01.006	journal	June 2009
The Path Forward for Biofuels and Biomaterials Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H. Science, Vol. 311, Issue 5760, p. 484-489 https://doi.org/10.1126/science.1114736	journal	January 2006
A novel cellulase free alkaliphilic xylanase from alkali tolerant Penicillium citrinum: production, purification and characterization Dutta, T.; Sengupta, R.; Sahoo, R. Letters in Applied Microbiology, Vol. 44, Issue 2 https://doi.org/10.1111/j.1472-765X.2006.02042.x	journal	February 2007
Hydrolysis of soybean isoflavone glycosides by a thermostable β-glucosidase from Paecilomyces thermophila Yang, Shaoqing; Wang, Lijun; Yan, Qiaojuan Food Chemistry, Vol. 115, Issue 4 https://doi.org/10.1016/j.foodchem.2009.01.038	journal	August 2009
Cell-associated acid β-xylosidase production by Penicillium sclerotiorum Knob, Adriana; Carmona, Eleonora Cano New Biotechnology, Vol. 26, Issue 1-2 https://doi.org/10.1016/j.nbt.2009.03.002	journal	October 2009
Effect of biomass concentration and inoculum source on the rate of anaerobic cellulose solubilization Jensen, Paul D.; Hardin, Matthew T.; Clarke, William P. Bioresource Technology, Vol. 100, Issue 21 https://doi.org/10.1016/j.biortech.2009.05.018	journal	November 2009
Cellulose: Fascinating Biopolymer and Sustainable Raw Material Klemm, Dieter; Heublein, Brigitte; Fink, Hans-Peter Angewandte Chemie International Edition, Vol. 44, Issue 22 https://doi.org/10.1002/anie.200460587	journal	May 2005
Surface activities, biodegradability and antimicrobial properties of n-alkyl glucosides, mannosides and galactosides Matsumura, Shuichi; Imai, Kazuyasu; Yoshikawa, Sadao Journal of the American Oil Chemists' Society, Vol. 67, Issue 12 https://doi.org/10.1007/BF02541865	journal	December 1990
Effect of Reduction in Yeast and Enzyme Concentrations in a Simultaneous- Saccharification-and-Fermentation–Based Bioethanol Process: Technical and Economic Evaluation Wingren, Anders; Galbe, Mats; Roslander, Christian Applied Biochemistry and Biotechnology, Vol. 122, Issue 1-3 https://doi.org/10.1385/ABAB:122:1-3:0485	journal	January 2005
Kinetics of solka floc cellulose hydrolysis bytrichoderma viride cellulase Howell, J. A.; Stuck, J. D. Biotechnology and Bioengineering, Vol. 17, Issue 6 https://doi.org/10.1002/bit.260170608	journal	June 1975
Isolation and characterization of a β-glucosidase from Penicillium decumbens and improving hydrolysis of corncob residue by using it as cellulase supplementation Chen, Mei; Qin, Yuqi; Liu, Ziyong Enzyme and Microbial Technology, Vol. 46, Issue 6 https://doi.org/10.1016/j.enzmictec.2010.01.008	journal	May 2010
Identification, purification and characterization of β-glucosidase from apple seed as a novel catalyst for synthesis of O-glucosides Yu, Hui-Lei; Xu, Jian-He; Lu, Wen-Ya Enzyme and Microbial Technology, Vol. 40, Issue 2 https://doi.org/10.1016/j.enzmictec.2006.05.004	journal	January 2007
A novel family of hemicellulolytic α-glucuronidase Ryabova, Olena; Vršanská, Mária; Kaneko, Satoshi FEBS Letters, Vol. 583, Issue 9 https://doi.org/10.1016/j.febslet.2009.03.057	journal	April 2009
What is (and is not) vital to advancing cellulosic ethanol Wyman, Charles E. Trends in Biotechnology, Vol. 25, Issue 4 https://doi.org/10.1016/j.tibtech.2007.02.009	journal	April 2007
Factors affecting cellulose hydrolysis and the potential of enzyme recycle to enhance the efficiency of an integrated wood to ethanol process Gregg, David J.; Saddler, John N. Biotechnology and Bioengineering, Vol. 51, Issue 4 https://doi.org/10.1002/(SICI)1097-0290(19960820)51:4<375::AID-BIT1>3.3.CO;2-G	journal	August 1996
Hydrolysis of lignocellulosic materials for ethanol production: a review Sun, Ye; Cheng, Jiayang Bioresource Technology, Vol. 83, Issue 1, p. 1-11 https://doi.org/10.1016/S0960-8524(01)00212-7	journal	May 2002
Xylanase production by fungal mixed culture solid substrate fermentation on sugar cane bagasse Gutierrez-Correa, Marcel; Tengerdy, Robert P. Biotechnology Letters, Vol. 20, Issue 1, p. 45-47 https://doi.org/10.1023/A:1005379030092	journal	January 1998
An improved method to directly estimate cellulase adsorption on biomass solids Kumar, Rajeev; Wyman, Charles E. Enzyme and Microbial Technology, Vol. 42, Issue 5 https://doi.org/10.1016/j.enzmictec.2007.12.005	journal	April 2008
Microbial xylanases and their industrial applications: a review Beg, Q. K.; Kapoor, M.; Mahajan, L. Applied Microbiology and Biotechnology, Vol. 56, Issue 3-4 https://doi.org/10.1007/s002530100704	journal	August 2001
Purification and properties of an extracellular β-xylosidase from a newly isolated Fusarium proliferatum Saha, Badal C. Bioresource Technology, Vol. 90, Issue 1 https://doi.org/10.1016/S0960-8524(03)00098-1	journal	October 2003
Expression in Trichoderma reesei and characterisation of a thermostable family 3 β-glucosidase from the moderately thermophilic fungus Talaromyces emersonii Murray, Patrick; Aro, Nina; Collins, Catherine Protein Expression and Purification, Vol. 38, Issue 2, p. 248-257 https://doi.org/10.1016/j.pep.2004.08.006	journal	December 2004
Xylanase Production by Penicillium canescens 10-10c in Solid-State Fermentation Bakri, Yasser; Jacques, Philippe; Thonart, Philippe Applied Biochemistry and Biotechnology, Vol. 108, Issue 1-3 https://doi.org/10.1385/ABAB:108:1-3:737	journal	January 2003
Applications of enzymes in synthetic carbohydrate chemistry Thiem, Joachim FEMS Microbiology Reviews, Vol. 16, Issue 2-3 https://doi.org/10.1111/j.1574-6976.1995.tb00166.x	journal	February 1995
β-Glucosidase Production by <I>Trichoderma reesei</I> Juhász, Tamás; Egyházi, Anita; Réczey, Kati Applied Biochemistry and Biotechnology, Vol. 121, Issue 1-3 https://doi.org/10.1385/ABAB:121:1-3:0243	journal	January 2005
Aspergillus Enzymes Involved in Degradation of Plant Cell Wall Polysaccharides de Vries, R. P.; Visser, J. Microbiology and Molecular Biology Reviews, Vol. 65, Issue 4 https://doi.org/10.1128/MMBR.65.4.497-522.2001	journal	December 2001
Purification and characterization of carboxymethylcellulase isolated from a marine bacterium, Bacillus subtilis subsp. subtilis A-53 Kim, Bo-Kyung; Lee, Bo-Hwa; Lee, Yoo-Jung Enzyme and Microbial Technology, Vol. 44, Issue 6-7 https://doi.org/10.1016/j.enzmictec.2009.02.005	journal	June 2009
Microbial xylanolytic systems Biely, Peter Trends in Biotechnology, Vol. 3, Issue 11 https://doi.org/10.1016/0167-7799(85)90004-6	journal	November 1985
Microbial β-Glucosidases: Cloning, Properties, and Applications Bhatia, Yukti; Mishra, Saroj; Bisaria, V. S. Critical Reviews in Biotechnology, Vol. 22, Issue 4, p. 375-407 https://doi.org/10.1080/07388550290789568	journal	January 2002
Bacterial Reduction of Perchlorate and Nitrate in Water Herman, David C.; Frankenberger, William T. Journal of Environment Quality, Vol. 28, Issue 3 https://doi.org/10.2134/jeq1999.00472425002800030036x	journal	January 1999
Properties of a xylanase from Streptomyces matensis being suitable for xylooligosaccharides production Yan, Qiaojuan; Hao, Shanshan; Jiang, Zhengqiang Journal of Molecular Catalysis B: Enzymatic, Vol. 58, Issue 1-4 https://doi.org/10.1016/j.molcatb.2008.11.010	journal	June 2009
Kinetics of batch single cell protein production from rice polishings with Candida utilis in continuously aerated tank reactors Rajoka, M. Ibrahim; Khan, Sohail Hassan; Jabbar, M. A. Bioresource Technology, Vol. 97, Issue 15 https://doi.org/10.1016/j.biortech.2005.08.019	journal	October 2006
Effect of xylanase supplementation of cellulase on digestion of corn stover solids prepared by leading pretreatment technologies Kumar, Rajeev; Wyman, Charles E. Bioresource Technology, Vol. 100, Issue 18 https://doi.org/10.1016/j.biortech.2008.11.057	journal	September 2009
A xylose-tolerant β-xylosidase from Paecilomyces thermophila: Characterization and its co-action with the endogenous xylanase Yan, Q. J.; Wang, L.; Jiang, Z. Q. Bioresource Technology, Vol. 99, Issue 13 https://doi.org/10.1016/j.biortech.2007.11.033	journal	September 2008
A thermotolerant β-glucosidase isolated from an endophytic fungi, Periconia sp., with a possible use for biomass conversion to sugars Harnpicharnchai, Piyanun; Champreda, Verawat; Sornlake, Warasirin Protein Expression and Purification, Vol. 67, Issue 2, p. 61-69 https://doi.org/10.1016/j.pep.2008.05.022	journal	October 2009
Isolation of cellulose-hydrolytic bacteria and applications of the cellulolytic enzymes for cellulosic biohydrogen production Lo, Yung-Chung; Saratale, Ganesh D.; Chen, Wen-Ming Enzyme and Microbial Technology, Vol. 44, Issue 6-7 https://doi.org/10.1016/j.enzmictec.2009.03.002	journal	June 2009
Effect of additives on the digestibility of corn stover solids following pretreatment by leading technologies Kumar, Rajeev; Wyman, Charles E. Biotechnology and Bioengineering, Vol. 102, Issue 6 https://doi.org/10.1002/bit.22203	journal	April 2009
Enhancement of enzymatic saccharification of cellulose by cellulose dissolution pretreatments Kuo, Chia-Hung; Lee, Cheng-Kang Carbohydrate Polymers, Vol. 77, Issue 1 https://doi.org/10.1016/j.carbpol.2008.12.003	journal	May 2009
Pretreatment: the key to unlocking low-cost cellulosic ethanol Yang, Bin; Wyman, Charles E. Biofuels, Bioproducts and Biorefining, Vol. 2, Issue 1 https://doi.org/10.1002/bbb.49	journal	January 2008
Screening and identification of xylanase-producing bacteria from Thai soils Kinegam, Saowapar; Tanasupawat, Somboon; Akaracharanya, Ancharida The Journal of General and Applied Microbiology, Vol. 53, Issue 1 https://doi.org/10.2323/jgam.53.57	journal	January 2007
The bgl1 gene of Trichoderma reesei QM 9414 encodes an extracellular, cellulose-inducible β-glucosidase involved in cellulase induction by sophorose Mach, Robert L.; Seiboth, Bernhard; Myasnikov, Andrey Molecular Microbiology, Vol. 16, Issue 4, p. 687-697 https://doi.org/10.1111/j.1365-2958.1995.tb02430.x	journal	May 1995
Formation and Location of 1,4-β-Glucanases and 1,4-β-Glucosidases from Penicillium janthinellum Rapp, Peter; Grote, Elisabeth; Wagner, Fritz Applied and Environmental Microbiology, Vol. 41, Issue 4 https://doi.org/10.1128/AEM.41.4.857-866.1981	journal	January 1981
Microbiological Procedures for Biodegradation Research Focht, Dennis D. Methods of Soil Analysis: Part 2 Microbiological and Biochemical Properties https://doi.org/10.2136/sssabookser5.2.c20	book	September 2018

Cited By (1)

Canola meal as a novel substrate for β-glucosidase production by Trichoderma viride: application of the crude extract to biomass saccharification Almeida, J. M.; Lima, V. A.; Giloni-Lima, P. C. Bioprocess and Biosystems Engineering, Vol. 38, Issue 10 https://doi.org/10.1007/s00449-015-1429-0	journal	June 2015

Similar Records

Selection and molecular characterization of cellulolytic–xylanolytic fungi from surface soil-biomass mixtures from Black Belt sites

Journal Article · Mon Jun 01 00:00:00 EDT 2015 · Microbiological Research · OSTI ID:1419492

Okeke, Benedict C.; Hall, Rosine W.; Nanjundaswamy, Ananda; +4 more

Activity-based protein profiling of secreted cellulolytic enzyme activity dynamics in Trichoderma reesei QM6a, NG14, and RUT-C30

Journal Article · Sun Dec 01 00:00:00 EST 2013 · Molecular Biosystems, 9(12):2992-3000 · OSTI ID:1419492

Anderson, Lindsey N.; Culley, David E.; Hofstad, Beth A.; +7 more

Enhanced production of cellulase, hemicellulase, and β-glucosidase by Trichoderma reesei (Rut C-30)

Journal Article · Sat Aug 01 00:00:00 EDT 1981 · Biotechnology and Bioengineering · OSTI ID:1419492

Tangnu, S. Kishen; Blanch, Harvey W.; Wilke, Charles R.

Related Subjects

09 BIOMASS FUELS
Cellulase
xylanase
β-glucosidase
β-xylosidae
biomass saccharides
lignocellulose
bioprocessing
biofuel

Title: Cellulolytic and Xylanolytic Potential of High β-Glucosidase-Producing Trichoderma from Decaying Biomass

Citation Formats

References (55)

Cited By (1)

Similar Records

Related Subjects