Enhanced production of cellulase, hemicellulase, and β-glucosidase by Trichoderma reesei (Rut C-30)

Journal Article · Sat Aug 01 04:00:00 EDT 1981 · Biotechnology and Bioengineering

DOI:https://doi.org/10.1002/bit.260230811· OSTI ID:1072216

Tangnu, S. Kishen ^[1]; Blanch, Harvey W. ^[1]; Wilke, Charles R. ^[1]

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1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States). Dept. of Chemical Engineering

In this paper, the Production of cellulases and Hemicellulases was studied with Trichoderma reesei Rut C-30, This organism produced, together with high cellulase activities, considerable amounts of xylanases and β-glucosidase. Three cellulose concentration (1, 2.5, and 5.0%) were examined to determined the maximum levels of cellulase activity obtainable in submerged culture. Temperature and pH profiling was used to increase cell mass to maximum levels within two days and thereby enhancing fermentor productivity at higher substrate levels. Finally, the effect of temperature, pH, Tween-80 concentration, carbon sources, and substrate concentration on the ration of mycelial growth and extracellulose enzyme production are described.

• Research Organization: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: USDOE
• Contributing Organization: Univ. of California, Berkeley, CA (United States)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 1072216
• Report Number(s): LBL--11074
• Journal Information: Biotechnology and Bioengineering, Journal Name: Biotechnology and Bioengineering Journal Issue: 8 Vol. 23; ISSN 0006-3592
• Publisher: Wiley
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES