Microbial Potential for Ecosystem N Loss Is Increased by Experimental N Deposition

Freedman, Zachary B.; Upchurch, Rima A.; Zak, Donald R.; Miki, Takeshi

doi:10.1371/journal.pone.0164531

Title: Microbial Potential for Ecosystem N Loss Is Increased by Experimental N Deposition

Journal Article · Thu Oct 13 00:00:00 EDT 2016 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0164531· OSTI ID:1377882

^[1]; Upchurch, Rima A. ^[2]; Zak, Donald R. ^[2]; Miki, Takeshi ^[3]

Univ. of Michigan, Ann Arbor, MI (United States); West Virginia Univ., Morgantown, WV (United States)
Univ. of Michigan, Ann Arbor, MI (United States)
National Taiwan Univ. (Taiwan)

Fossil fuel combustion and fertilizer use has increased the amount of biologically available N entering terrestrial ecosystems. Nonetheless, our understanding of how anthropogenic N may alter the physiological mechanisms by which soil microorganisms cycle N in soil is still developing. Here, we applied shotgun metagenomics to a replicated long-term field experiment to determine how two decades of experimental N deposition, at a rate expected by mid-century, has affected the genetic potential of the soil microbial community to cycle N in soils. Experimental N deposition lead to a significant and persistent increase in functional assemblages mediating N cycle transformations associated with ecosystem N loss (i.e., denitrification and nitrification), whereas functional assemblages associated with N input and retention (i.e., N fixation and microbial N assimilation) were less positively affected. Furthermore, the abundance and composition of microbial taxa, as well as functional assemblages involved in housekeeping functions (i.e., DNA replication) were unaffected by experimental N deposition. Here taken together, our results suggest that functional genes and gene pathways associated with ecosystem N loss have been favored by experimental N deposition, which may represent a genetic mechanism fostering increased N loss as anthropogenic N deposition increases in the future.

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Research Organization:: West Virginia Univ., Morgantown, WV (United States)

Sponsoring Organization:: USDOE; Biological and Environmental Research (BER)

OSTI ID:: 1377882

Journal Information:: PLoS ONE, Vol. 11, Issue 10; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 9 works

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