The carbon starvation response of the ectomycorrhizal fungus Paxillus involutus

Ellstrom, Magnus; Shah, Firoz; Johansson, Tomas; Ahren, Dag; Persson, Per; Tunlid, Anders

doi:10.1093/femsec/fiv027

Title: The carbon starvation response of the ectomycorrhizal fungus Paxillus involutus

Journal Article · Mon Mar 16 00:00:00 EDT 2015 · FEMS Microbiology Ecology (Online)

DOI:https://doi.org/10.1093/femsec/fiv027· OSTI ID:1343065

Ellstrom, Magnus ^[1]; Shah, Firoz ^[2]; Johansson, Tomas ^[2]; Ahren, Dag ^[3]; Persson, Per ^[1]; Tunlid, Anders ^[2]

Lund Univ. (Sweden). Dept. of Biology. Microbial Ecology. Centre for Environmental and Climate Research (CEC)
Lund Univ. (Sweden). Dept. of Biology. Microbial Ecology
Lund Univ. (Sweden). Dept. of Biology. Microbial Ecology. Bioinformatics Infrastructure for Life Sciences (BILS)

The amounts of carbon allocated to the fungal partner in ectomycorrhizal associations can vary substantially depending on the plant growth and the soil nutrient conditions, and the fungus may frequently be confronted with limitations in carbon. We used chemical analysis and transcriptome profiling to examine the physiological response of the ectomycorrhizal fungus Paxillus involutus to carbon starvation during axenic cultivation. Carbon starvation induced a decrease in the biomass. Concomitantly, ammonium, cell wall material (chitin) and proteolytic enzymes were released into the medium, which suggest autolysis. Compared with the transcriptome of actively growing hyphae, about 45% of the transcripts analyzed were differentially regulated during C-starvation. Induced during starvation were transcripts encoding extracellular enzymes such as peptidases, chitinases and laccases. In parallel, transcripts of N-transporters were upregulated, which suggest that some of the released nitrogen compounds were re-assimilated by the mycelium. The observed changes suggest that the carbon starvation response in P. involutus is associated with complex cellular changes that involves autolysis, recycling of intracellular compounds by autophagy and reabsorption of the extracellular released material. The study provides molecular markers that can be used to examine the role of autolysis for the turnover and survival of the ectomycorrhizal mycelium in soils.

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Research Organization:: Lund Univ. (Sweden); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); Swedish Research Council (VR)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1343065

Journal Information:: FEMS Microbiology Ecology (Online), Vol. 91, Issue 4; ISSN 1574-6941

Publisher:: Federation of European Microbiological SocietiesCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 19 works

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