The molecular components of the extracellular protein-degradation pathways of the ectomycorrhizal fungus Paxillus involutus

Shah, Firoz; Rineau, Francois; Canbäck, Björn; Johansson, Tomas; Tunlid, Anders

doi:10.1111/nph.12425

The molecular components of the extracellular protein-degradation pathways of the ectomycorrhizal fungus Paxillus involutus

Journal Article · Wed Jul 31 00:00:00 EDT 2013 · New Phytologist

DOI:https://doi.org/10.1111/nph.12425· OSTI ID:1625912

Shah, Firoz ^[1]; Rineau, Francois ^[2]; Canbäck, Björn ^[2]; Johansson, Tomas ^[2]; Tunlid, Anders ^[2]

Lund Univ. (Sweden); DOE/OSTI
Lund Univ. (Sweden)

Proteins contribute to a major part of the organic nitrogen (N) in forest soils. This N is mobilized and becomes available to trees as a result of the depolymerizing activities of symbiotic ectomycorrhizal fungi. The mechanisms by which these fungi depolymerize proteins and assimilate the released N are poorly characterized. Biochemical analysis and transcriptome profiling were performed to examine the proteolytic machinery and the uptake system of the ectomycorrhizal basidiomycete Paxillus involutus during the assimilation of organic N from various protein sources and extracts of organic matter. All substrates induced secretion of peptidase activity with an acidic pH optimum, mostly contributed by aspartic peptidases. The peptidase activity was transiently repressed by ammonium. Transcriptional analysis revealed a large number of extracellular endo- and exopeptidases. The expression levels of these peptidases were regulated in parallel with transporters and enzymes involved in the assimilation and metabolism of the released peptides and amino acids. For the first time the molecular components of the protein degradation pathways of an ectomycorrhizal fungus are described. The data suggest that the transcripts encoding these components are regulated in response to the chemical properties and the availability of the protein substrates.

View Accepted Manuscript (DOE)

Research Organization:: Lund University (Sweden)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1625912

Journal Information:: New Phytologist, Journal Name: New Phytologist Journal Issue: 3 Vol. 200; ISSN 0028-646X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Evidences on the Ability of Mycorrhizal Genus Piloderma to Use Organic Nitrogen and Deliver It to Scots Pine Heinonsalo, Jussi; Sun, Hui; Santalahti, Minna PLOS ONE, Vol. 10, Issue 7 https://doi.org/10.1371/journal.pone.0131561	journal	July 2015
Comparative Analysis of Secretomes from Ectomycorrhizal Fungi with an Emphasis on Small-Secreted Proteins Garcia, Kevin; Ané, Jean-Michel Frontiers in Microbiology, Vol. 7 https://doi.org/10.3389/fmicb.2016.01734	journal	November 2016
A dipeptide transporter from the arbuscular mycorrhizal fungus Rhizophagus irregularis is upregulated in the intraradical phase Belmondo, Simone; Fiorilli, Valentina; PÃ©rez-Tienda, Jacob Frontiers in Plant Science, Vol. 5 https://doi.org/10.3389/fpls.2014.00436	journal	September 2014
Digging Deeper: In Search of the Mechanisms of Carbon and Nitrogen Exchange in Ectomycorrhizal Symbioses Stuart, Emiko K.; Plett, Krista L. Frontiers in Plant Science, Vol. 10 https://doi.org/10.3389/fpls.2019.01658	journal	January 2020
Comparative genomics and expression levels of hydrophobins from eight mycorrhizal genomes Rineau, F.; Lmalem, H.; Ahren, D. Mycorrhiza, Vol. 27, Issue 4 https://doi.org/10.1007/s00572-016-0758-4	journal	January 2017
Regulation of fungal decomposition at single-cell level Op De Beeck, Michiel; Troein, Carl; Siregar, Syahril The ISME Journal, Vol. 14, Issue 4 https://doi.org/10.1038/s41396-019-0583-9	journal	January 2020
Mineral surface-reactive metabolites secreted during fungal decomposition contribute to the formation of soil organic matter: Fungal metabolites for soil carbon stabilization Wang, Tao; Tian, Zhaomo; Bengtson, Per Environmental Microbiology, Vol. 19, Issue 12 https://doi.org/10.1111/1462-2920.13990	journal	December 2017
First evidences that the ectomycorrhizal fungus Paxillus involutus mobilizes nitrogen and carbon from saprotrophic fungus necromass: Do ectomycorrhizal fungi degrade brown rot fungi? Akroume, Emila; Maillard, François; Bach, Cyrille Environmental Microbiology, Vol. 21, Issue 1 https://doi.org/10.1111/1462-2920.14440	journal	November 2018
Scavenging organic nitrogen and remodelling lipid metabolism are key survival strategies adopted by the endophytic fungi, Serendipita vermifera and Serendipita bescii to alleviate nitrogen and phosphorous starvation in vitro Ray, Prasun; Abraham, Paul E.; Guo, Yingqing Environmental Microbiology Reports, Vol. 11, Issue 4 https://doi.org/10.1111/1758-2229.12757	journal	May 2019
Fungal and plant gene expression in the Tulasnella calospora - Serapias vomeracea symbiosis provides clues about nitrogen pathways in orchid mycorrhizas Fochi, Valeria; Chitarra, Walter; Kohler, Annegret New Phytologist, Vol. 213, Issue 1 https://doi.org/10.1111/nph.14279	journal	November 2016
Fenton reaction facilitates organic nitrogen acquisition by an ectomycorrhizal fungus Op De Beeck, Michiel; Troein, Carl; Peterson, Carsten New Phytologist, Vol. 218, Issue 1 https://doi.org/10.1111/nph.14971	journal	January 2018
Interaction between tannins and fungal necromass stabilizes fungal residues in boreal forest soils Adamczyk, Bartosz; Sietiö, Outi‐Maaria; Biasi, Christina New Phytologist, Vol. 223, Issue 1 https://doi.org/10.1111/nph.15729	journal	February 2019
Involutin Is an Fe ³⁺ Reductant Secreted by the Ectomycorrhizal Fungus Paxillus involutus during Fenton-Based Decomposition of Organic Matter Shah, Firoz; Schwenk, Daniel; Nicolás, César Applied and Environmental Microbiology, Vol. 81, Issue 24 https://doi.org/10.1128/aem.02312-15	journal	October 2015
Polyporales Brown Rot Species Fomitopsis pinicola : Enzyme Activity Profiles, Oxalic Acid Production, and Fe ³⁺ -Reducing Metabolite Secretion Shah, Firoz; Mali, Tuulia; Lundell, Taina K. Applied and Environmental Microbiology, Vol. 84, Issue 8 https://doi.org/10.1128/aem.02662-17	journal	February 2018
Influence of Ammonium on Formation of Mineral-Associated Organic Carbon by an Ectomycorrhizal Fungus Wang, Tao; Tian, Zhaomo; Tunlid, Anders Applied and Environmental Microbiology, Vol. 85, Issue 10 https://doi.org/10.1128/aem.03007-18	journal	March 2019
Comparative Analysis of Secretomes from Ectomycorrhizal Fungi with an Emphasis on Small-Secreted Proteins Pellegrin, Clement; Morin, Emmanuelle; Martin, Francis M. Frontiers in Microbiology, Vol. 6 https://doi.org/10.3389/fmicb.2015.01278	journal	November 2015
Regulation of fungal decomposition at single-cell level De Beeck, Michiel Op; Troein, Carl; Siregar, Syahril EGU General Assembly 2021 (vEGU21) https://doi.org/10.5194/egusphere-egu21-8198	posted_content	April 2021

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