Photon counting phosphorescence lifetime imaging with TimepixCam

Hirvonen, Liisa M.; Fisher-Levine, Merlin; Suhling, Klaus; Nomerotski, Andrei

doi:10.1063/1.4973717

Title: Photon counting phosphorescence lifetime imaging with TimepixCam

Journal Article · Thu Jan 12 00:00:00 EST 2017 · Review of Scientific Instruments

DOI:https://doi.org/10.1063/1.4973717· OSTI ID:1341684

^[1]; Fisher-Levine, Merlin ^[2]; Suhling, Klaus ^[1]; Nomerotski, Andrei ^[3]

King's College London, London (United Kingdom). Dept. of Physics
Brookhaven National Lab. (BNL), Upton, NY (United States); Peyton Hall, Princeton, NJ (United States). Dept. of Astrophysical Sciences
Brookhaven National Lab. (BNL), Upton, NY (United States)

TimepixCam is a novel fast optical imager based on an optimized silicon pixel sensor with a thin entrance window, and read out by a Timepix ASIC. The 256 x 256 pixel sensor has a time resolution of 15 ns at a sustained frame rate of 10 Hz. We used this sensor in combination with an image intensifier for wide-field time-correlated single photon counting (TCSPC) imaging. We have characterised the photon detection capabilities of this detector system, and employed it on a wide-field epifluorescence microscope to map phosphorescence decays of various iridium complexes with lifetimes of about 1 μs in 200 μm diameter polystyrene beads.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Brookhaven National Lab. (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), High Energy Physics (HEP)

Grant/Contract Number:: SC0012704; K015664; 13-006

OSTI ID:: 1341684

Report Number(s):: BNL-113413-2017-JA; KA2301020; TRN: US1700988

Journal Information:: Review of Scientific Instruments, Vol. 88, Issue 1; ISSN 0034-6748

Publisher:: American Institute of Physics (AIP)Copyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 25 works

Citation information provided by
Web of Science

References (37)

Single-photon generation and detection Buller, G. S.; Collins, R. J. Measurement Science and Technology, Vol. 21, Issue 1 https://doi.org/10.1088/0957-0233/21/1/012002	journal	November 2009
Single-photon detectors for optical quantum information applications Hadfield, Robert H. Nature Photonics, Vol. 3, Issue 12 https://doi.org/10.1038/nphoton.2009.230	journal	December 2009
Invited Review Article: Single-photon sources and detectors Eisaman, M. D.; Fan, J.; Migdall, A. Review of Scientific Instruments, Vol. 82, Issue 7 https://doi.org/10.1063/1.3610677	journal	July 2011
Lighting the way to see inside the live cell with luminescent transition metal complexes Baggaley, Elizabeth; Weinstein, Julia A.; Williams, J. A. Gareth Coordination Chemistry Reviews, Vol. 256, Issue 15-16 https://doi.org/10.1016/j.ccr.2012.03.018	journal	August 2012
Optical probes and techniques for O2 measurement in live cells and tissue Dmitriev, Ruslan I.; Papkovsky, Dmitri B. Cellular and Molecular Life Sciences, Vol. 69, Issue 12 https://doi.org/10.1007/s00018-011-0914-0	journal	January 2012
Hydrodynamic Radii of Ranibizumab, Aflibercept and Bevacizumab Measured by Time-Resolved Phosphorescence Anisotropy Hirvonen, Liisa M.; Fruhwirth, Gilbert O.; Srikantha, Nishanthan Pharmaceutical Research, Vol. 33, Issue 8 https://doi.org/10.1007/s11095-016-1940-2	journal	May 2016
Metal-ligand complexes as a new class of long-lived fluorophores for protein hydrodynamics Terpetschnig, E.; Szmacinski, H.; Malak, H. Biophysical Journal, Vol. 68, Issue 1 https://doi.org/10.1016/S0006-3495(95)80193-1	journal	January 1995
Luminescent barometry in wind tunnels Kavandi, Janet; Callis, James; Gouterman, Martin Review of Scientific Instruments, Vol. 61, Issue 11 https://doi.org/10.1063/1.1141632	journal	November 1990
Remote thermometry with thermographic phosphors: Instrumentation and applications Allison, S. W.; Gillies, G. T. Review of Scientific Instruments, Vol. 68, Issue 7 https://doi.org/10.1063/1.1148174	journal	July 1997
Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods Gratton, Enrico Journal of Biomedical Optics, Vol. 8, Issue 3 https://doi.org/10.1117/1.1586704	journal	July 2003
Theoretical investigation of the signal-to-noise ratio in fluorescence lifetime imaging Philip, Johan; Carlsson, Kjell Journal of the Optical Society of America A, Vol. 20, Issue 2 https://doi.org/10.1364/JOSAA.20.000368	journal	January 2003
Optimizing frequency-domain fluorescence lifetime sensing for high-throughput applications: photon economy and acquisition speed Esposito, Alessandro; Gerritsen, Hans C.; Wouters, Fred S. Journal of the Optical Society of America A, Vol. 24, Issue 10 https://doi.org/10.1364/JOSAA.24.003261	journal	January 2007
Fluorescence lifetime imaging in scanning microscopes: acquisition speed, photon economy and lifetime resolution: FAST FLUORESCENCE LIFETIME IMAGING Gerritsen, H. C.; Asselbergs, M. A. H.; Agronskaia, A. V. Journal of Microscopy, Vol. 206, Issue 3 https://doi.org/10.1046/j.1365-2818.2002.01031.x	journal	June 2002
Wide-field TCSPC: methods and applications Hirvonen, Liisa M.; Suhling, Klaus Measurement Science and Technology, Vol. 28, Issue 1 https://doi.org/10.1088/1361-6501/28/1/012003	journal	December 2016
Wide-field time-correlated single-photon counting (TCSPC) lifetime microscopy with microsecond time resolution Hirvonen, Liisa M.; Festy, Frederic; Suhling, Klaus Optics Letters, Vol. 39, Issue 19 https://doi.org/10.1364/OL.39.005602	journal	January 2014
Sub- μ s time resolution in wide-field time-correlated single photon counting microscopy obtained from the photon event phosphor decay Hirvonen, Liisa M.; Petrášek, Zdeněk; Beeby, Andrew New Journal of Physics, Vol. 17, Issue 2 https://doi.org/10.1088/1367-2630/17/2/023032	journal	February 2015
Review of hybrid pixel detector readout ASICs for spectroscopic X-ray imaging Ballabriga, R.; Alozy, J.; Campbell, M. Journal of Instrumentation, Vol. 11, Issue 01 https://doi.org/10.1088/1748-0221/11/01/P01007	journal	January 2016
Temporal pixel multiplexing for simultaneous high-speed, high-resolution imaging Bub, Gil; Tecza, Matthias; Helmes, Michiel Nature Methods, Vol. 7, Issue 3 https://doi.org/10.1038/nmeth.1429	journal	February 2010
Timepix, a 65k programmable pixel readout chip for arrival time, energy and/or photon counting measurements Llopart, X.; Ballabriga, R.; Campbell, M. Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 581, Issue 1-2 https://doi.org/10.1016/j.nima.2007.08.079	journal	October 2007
Fast, High Resolution Mass Spectrometry Imaging Using a Medipix Pixelated Detector Jungmann, Julia H.; MacAleese, Luke; Buijs, Ronald Journal of the American Society for Mass Spectrometry, Vol. 21, Issue 12 https://doi.org/10.1016/j.jasms.2010.08.014	journal	December 2010
Optically sensitive MCP image tube with a Medipix2 ASIC readout Vallerga, John; McPhate, Jason; Tremsin, Anton SPIE Astronomical Telescopes + Instrumentation, SPIE Proceedings https://doi.org/10.1117/12.790600	conference	August 2008
Status of the Timepix MCP-HPD development Tick, T.; Campbell, M.; Michel, T. Journal of Instrumentation, Vol. 5, Issue 12 https://doi.org/10.1088/1748-0221/5/12/C12020	journal	December 2010
Optical MCP image tube with a quad Timepix readout: initial performance characterization Vallerga, J.; Tremsin, A.; DeFazio, J. Journal of Instrumentation, Vol. 9, Issue 05 https://doi.org/10.1088/1748-0221/9/05/C05055	journal	May 2014
TimepixCam: a fast optical imager with time-stamping Fisher-Levine, M.; Nomerotski, A. Journal of Instrumentation, Vol. 11, Issue 03 https://doi.org/10.1088/1748-0221/11/03/C03016	journal	March 2016
Characterization of TimepixCam, a fast imager for the time-stamping of optical photons Nomerotski, Andrei; Chakaberia, I.; Fisher-Levine, M. Journal of Instrumentation, Vol. 12, Issue 01 https://doi.org/10.1088/1748-0221/12/01/C01017	journal	January 2017
Multiphoton time-domain fluorescence lifetime imaging microscopy: practical application to protein–protein interactions using global analysis Barber, P. R.; Ameer-Beg, S. M.; Gilbey, J. Journal of The Royal Society Interface, Vol. 6, Issue suppl_1 https://doi.org/10.1098/rsif.2008.0451.focus	journal	August 2008
Manipulating Charge-Transfer Character with Electron-Withdrawing Main-Group Moieties for the Color Tuning of Iridium Electrophosphors Zhou, Guijiang; Ho, Cheuk-Lam; Wong, Wai-Yeung Advanced Functional Materials, Vol. 18, Issue 3 https://doi.org/10.1002/adfm.200700719	journal	February 2008
Tuning the emission of cyclometalated iridium complexes by simple ligand modification Beeby, Andrew; Bettington, Sylvia; Samuel, Ifor D. W. Journal of Materials Chemistry, Vol. 13, Issue 1 https://doi.org/10.1039/b207502d	journal	November 2002
Photon budget analysis for fluorescence lifetime imaging microscopy Zhao, Qiaole; Young, Ian T.; de Jong, Jan Geert Sander Journal of Biomedical Optics, Vol. 16, Issue 8 https://doi.org/10.1117/1.3608997	journal	January 2011
Development of new photon-counting detectors for single-molecule fluorescence microscopy Michalet, X.; Colyer, R. A.; Scalia, G. Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 368, Issue 1611 https://doi.org/10.1098/rstb.2012.0035	journal	February 2013
Cross strip anode readouts for large format, photon counting microchannel plate detectors: developing flight qualified prototypes of the detector and electronics Vallerga, John; Raffanti, Rick; Cooney, Michael SPIE Astronomical Telescopes + Instrumentation, SPIE Proceedings https://doi.org/10.1117/12.2058412	conference	July 2014
Picosecond wide-field time-correlated single photon counting fluorescence microscopy with a delay line anode detector Hirvonen, Liisa M.; Becker, Wolfgang; Milnes, James Applied Physics Letters, Vol. 109, Issue 7 https://doi.org/10.1063/1.4961054	journal	August 2016
A wide-field TCSPC FLIM system based on an MCP PMT with a delay-line anode Becker, Wolfgang; Hirvonen, Liisa M.; Milnes, James Review of Scientific Instruments, Vol. 87, Issue 9 https://doi.org/10.1063/1.4962864	journal	September 2016
05 billion events per second time correlated single photon counting using CMOS SPAD arrays Krstajić, Nikola; Poland, Simon; Levitt, James Optics Letters, Vol. 40, Issue 18 https://doi.org/10.1364/OL.40.004305	journal	January 2015
Single-photon imaging in complementary metal oxide semiconductor processes Charbon, E. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 372, Issue 2012 https://doi.org/10.1098/rsta.2013.0100	journal	March 2014
Timepix3: a 65K channel hybrid pixel readout chip with simultaneous ToA/ToT and sparse readout Poikela, T.; Plosila, J.; Westerlund, T. Journal of Instrumentation, Vol. 9, Issue 05 https://doi.org/10.1088/1748-0221/9/05/C05013	journal	May 2014
PImMS, a fast event-triggered monolithic pixel detector with storage of multiple timestamps John, J. J.; Brouard, M.; Clark, A. Journal of Instrumentation, Vol. 7, Issue 08 https://doi.org/10.1088/1748-0221/7/08/C08001	journal	August 2012

Cited By (3)

Coincidence velocity map imaging using Tpx3Cam, a time stamping optical camera with 1.5 ns timing resolution Zhao, Arthur; van Beuzekom, Martin; Bouwens, Bram Review of Scientific Instruments, Vol. 88, Issue 11 https://doi.org/10.1063/1.4996888	journal	November 2017
Time stamping of single optical photons with 10 ns resolution Chakaberia, Irakli; Cotlet, Mircea; Fisher-Levine, Merlin SPIE Commercial + Scientific Sensing and Imaging, SPIE Proceedings https://doi.org/10.1117/12.2262212	conference	May 2017
Coincidence velocity map imaging using Tpx3Cam, a time stamping optical camera with 1.5 ns timing resolution Zhao, Arthur; van Beuzekom, Martin; Bouwens, Bram arXiv https://doi.org/10.48550/arxiv.1707.06253	text	January 2017

Similar Records

Characterization of TimepixCam, a fast imager for the time-stamping of optical photons

Journal Article · Wed Jan 04 00:00:00 EST 2017 · Journal of Instrumentation · OSTI ID:1341684

Nomerotski, Andrei; Chakaberia, I.; Fisher-Levine, M.; +3 more

New luminescence lifetime macro-imager based on a Tpx3Cam optical camera

Journal Article · Thu Dec 05 00:00:00 EST 2019 · Biomedical Optics Express · OSTI ID:1341684

Sen, Rajannya; Hirvonen, Liisa M.; Zhdanov, Alexander; +4 more

Time-resolved ion imaging at free-electron lasers using TimepixCam

Journal Article · Tue Feb 20 00:00:00 EST 2018 · Journal of Synchrotron Radiation (Online) · OSTI ID:1341684

Fisher-Levine, Merlin; Boll, Rebecca; Ziaee, Farzaneh; +6 more

Related Subjects

79 ASTRONOMY AND ASTROPHYSICS
47 OTHER INSTRUMENTATION
Time-correlated single photon counting (TCSPC)
phosphorescence life-time imaging (PLIM)
microchannel plate (MCP)
Timepix ASIC

Title: Photon counting phosphorescence lifetime imaging with TimepixCam

Citation Formats

References (37)

Cited By (3)

Similar Records

Related Subjects