New luminescence lifetime macro-imager based on a Tpx3Cam optical camera

Sen, Rajannya; Hirvonen, Liisa M.; Zhdanov, Alexander; Svihra, Peter; Andersson-Engels, Stefan; Nomerotski, Andrei; Papkovsky, Dmitri

doi:10.1364/BOE.11.000077

New luminescence lifetime macro-imager based on a Tpx3Cam optical camera

Journal Article · Thu Dec 05 04:00:00 EST 2019 · Biomedical Optics Express

DOI:https://doi.org/10.1364/BOE.11.000077· OSTI ID:1571397

Sen, Rajannya; Hirvonen, Liisa M.; Zhdanov, Alexander; Svihra, Peter; ; Nomerotski, Andrei;

Properties of a novel ultra-fast optical imager, Tpx3Cam, were explored for macroscopic wide-field phosphorescent lifetime imaging (PLIM) applications. The camera is based on a novel optical sensor and Timepix3 readout chip with time resolution of 1.6 ns, recording of photon arrival time and time over threshold for each pixel, and readout rate of 80 megapixels per second. Here, we coupled the camera to an image intensifier, a 760 nm emission filter and a 50 mm lens, and with a super-bright 625nm LED providing pulsed excitation of a 18x18mm sample area. The resulting macro-imager with compact and rigid optical alignment of its main components was characterised using planar phosphorescent O₂ sensors and a resolution plate mask. Several acquisition and image processing algorithms were evaluated to optimise the system resolution and performance for the wide-field PLIM, followed by imaging a variety of phosphorescent samples. The new PLIM system looks promising, particularly for phosphorescence lifetime-based imaging of O₂ in various chemical and biological samples.

View Accepted Manuscript (DOE)

Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC), High Energy Physics (HEP) (SC-25); Science Foundation Ireland

Grant/Contract Number:: SC0012704

OSTI ID:: 1571397

Report Number(s):: BNL--212226-2019-JAAM

Journal Information:: Biomedical Optics Express, Journal Name: Biomedical Optics Express Journal Issue: 1 Vol. 11; ISSN 2156-7085

Publisher:: Optical Society of AmericaCopyright Statement

Country of Publication:: United States

Language:: English

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Tpx3Cam

New luminescence lifetime macro-imager based on a Tpx3Cam optical camera

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