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Title: Metabolic engineering of the shikimate pathway

Patent ·
OSTI ID:1338929
;

The present disclosure relates to engineered microorganisms that produce amino acids and amino acid intermediates. In particular, the disclosure relates to recombinant nucleic acids encoding operons that increase production of aromatic amino acids and the aromatic amino acid intermediate shikimate; microorganisms with increased production of aromatic amino acids and the aromatic amino acid intermediate shikimate; and methods related to the production of aromatic amino acids, the aromatic amino acid intermediate shikimate, and commodity chemicals derived therefrom.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-05CH11231
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
9,540,652
Application Number:
14/342,335
OSTI ID:
1338929
Resource Relation:
Patent File Date: 2012 Aug 31
Country of Publication:
United States
Language:
English

References (17)

Deletion of the aroK gene is essential for high shikimic acid accumulation through the shikimate pathway in E. coli journal September 2012
Metabolic Engineering of Escherichia coli for L-Tyrosine Production by Expression of Genes Coding for the Chorismate Mutase Domain of the Native Chorismate Mutase-Prephenate Dehydratase and a Cyclohexadienyl Dehydrogenase from Zymomonas mobilis journal March 2008
Development of genetically engineered bacteria for production of selected aromatic compounds patent April 2001
Phosphoenolpyruvate Availability and the Biosynthesis of Shikimic Acid journal June 2003
Shikimic acid production by a modified strain of E. coli (W3110.shik1) under phosphate-limited and carbon-limited conditions journal January 2005
Modulation of Phosphoenolpyruvate Synthase Expression Increases Shikimate Pathway Product Yields in E. coli journal December 2002
Towards bacterial strains overproducing l-tryptophan and other aromatics by metabolic engineering journal December 2005
Pathway engineering for production of aromatics in Escherichia coli: Confirmation of stoichiometric analysis by independent modulation of AroG, TktA, and Pps activities journal May 1995
Ensemble Modeling for Aromatic Production in Escherichia coli journal September 2009
Production of aromatic compounds in bacteria journal December 2009
From scratch to value: engineering Escherichia coli wild type cells to the production of l-phenylalanine and other fine chemicals derived from chorismate journal April 2007
Altered Glucose Transport and Shikimate Pathway Product Yields in E. coli journal October 2003
Metabolic engineering for microbial production of shikimic acid journal October 2003
L-Tyrosine production by deregulated strains of Escherichia coli journal January 2007
Combinatorial pathway analysis for improved L-tyrosine production in Escherichia coli: Identification of enzymatic bottlenecks by systematic gene overexpression journal March 2008
Metabolic transcription analysis of engineered Escherichia coli strains that overproduce L-phenylalanine journal January 2007
Metabolic engineering for the production of shikimic acid in an evolved Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system journal January 2010

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