Applications of Carboxylic Acid Reductases in Oleaginous Microbes
Conference
·
OSTI ID:1255241
Carboxylic acid reductases (CARs) are recently emerging reductive enzymes for the direct production of aldehydes from biologically-produced carboxylic acids. Recent work has demonstrated that these powerful enzymes are able to reduce a very broad range of volatile- to long-chain fatty acids as well as aromatic acids. Here, we express four CAR enzymes from different fungal origins to test their activity against fatty acids commonly produced in oleaginous microbes. These in vitro results will inform metabolic engineering strategies to conduct mild biological reduction of carboxylic acids in situ, which is conventionally done via hydrotreating catalysis at high temperatures and hydrogen pressures.
- Research Organization:
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Organization:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
- DOE Contract Number:
- AC36-08GO28308
- OSTI ID:
- 1255241
- Report Number(s):
- NREL/PO-5100-66304
- Resource Relation:
- Conference: Presented at the Symposium on Biotechnology for Fuels and Chemicals (SBFC), 25-28 April 2016, Baltimore, Maryland
- Country of Publication:
- United States
- Language:
- English
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