Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes

Sinha, Malavika; Weyda, István; Sørensen, Annette; Bruno, Kenneth S.; Ahring, Birgitte K.

doi:10.1186/s13568-016-0321-x

Title: Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes

Journal Article · Thu Jan 05 00:00:00 EST 2017 · AMB Express

DOI:https://doi.org/10.1186/s13568-016-0321-x· OSTI ID:1627036

Sinha, Malavika ^[1]; Weyda, István ^[2]; Sørensen, Annette ^[3]; Bruno, Kenneth S. ^[4]; Ahring, Birgitte K. ^[1]

Washington State Univ., Pullman, WA (United States). Sciences and Engineering Lab. Bioproducts
Aalborg Univ. Copenhagen (Denmark). Section for Sustainable Biotechnology
Washington State Univ., Pullman, WA (United States). Sciences and Engineering Lab. Bioproducts; Aalborg Univ. Copenhagen (Denmark). Section for Sustainable Biotechnology
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Chemical and Biological Process Development Group

In this study we describe the heterologous expression of the recently identified cyanobacterial pathway for long chain alkane biosynthesis, involving the reduction of fatty acyl-ACP to fatty aldehyde and the subsequent conversion of this into alkanes, in the filamentous fungus Aspergillus carbonarius ITEM 5010. Genes originating from Synechococcus elongatus strain PCC7942, encoding acyl-ACP/CoA reductase and aldehyde deformylating oxygenase enzymes, were successfully expressed in A. carbonarius, which lead to the production of pentadecane and heptadecane, alkanes that have not been previously produced by this fungus. Titers of 0.2, 0.5 and 2.7 mg/l pentadecane and 0.8, 1.6 and 10.2 mg/l heptadecane were achieved using glucose, Yeast malt and oatmeal media, respectively. Besides producing alkanes, we found elevated levels of internal free fatty acids and triglycerides in the alkane producing transformant. These findings can indicate that a yet unidentified, native fatty aldehyde dehydrogenase channels back the fatty aldehydes into the fatty acid metabolism, thus competing for substrate with the heterologously expressed fatty aldehyde deformylating oxygenase. These findings will potentially facilitate the future application of robust, fungal cell factories for the production of advanced biofuels from various substrates.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1627036

Journal Information:: AMB Express, Vol. 7, Issue 1; ISSN 2191-0855

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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The influence of fatty acid supply and aldehyde reductase deletion on cyanobacteria alkane generating pathway in Escherichia coli Wang, Juli; Yu, Haiying; Song, Xuejiao Journal of Industrial Microbiology & Biotechnology, Vol. 45, Issue 5 https://doi.org/10.1007/s10295-018-2032-6	journal	March 2018
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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Biotechnology & Applied Microbiology
Advanced biofuels
Alkane biosynthesis
Aspergillus carbonarius ITEM 5010
Fungal transformation

Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes Sinha, Malavika; Weyda, István; Sørensen, Annette https://doi.org/10.6084/m9.figshare.c.3658892 The current record is supplemented by this collection	collection	January 2017
Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes Sinha, Malavika; Weyda, István; Sørensen, Annette https://doi.org/10.6084/m9.figshare.c.3658892.v1 The current record is supplemented by this collection	collection	January 2017
MOESM1 of Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes Sinha, Malavika; Weyda, IstvĂĄn; SĂ¸Rensen, Annette https://doi.org/10.6084/m9.figshare.c.3658892_d1.v1 The current record is supplemented by this text	text	January 2017
MOESM1 of Alkane biosynthesis by Aspergillus carbonarius ITEM 5010 through heterologous expression of Synechococcus elongatus acyl-ACP/CoA reductase and aldehyde deformylating oxygenase genes Sinha, Malavika; Weyda, IstvĂĄn; SĂ¸Rensen, Annette https://doi.org/10.6084/m9.figshare.c.3658892_d1 The current record is supplemented by this text	text	January 2017