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Title: period -1 encodes an ATP-dependent RNA helicase that influences nutritional compensation of the Neurospora circadian clock

Journal Article · · Proceedings of the National Academy of Sciences of the United States of America
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  1. Geisel School of Medicine at Dartmouth, Hanover, NH (United States)
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
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Mutants in the period-1 (prd­-1) gene, characterized by a recessive allele, display a reduced growth rate and period lengthening of the developmental cycle controlled by the circadian clock. We refined the genetic location of prd­-1 and used whole genome sequencing to find the mutation defining it, confirming the identity of prd­-1 by rescuing the mutant circadian phenotype via transformation. PRD-1 is an RNA helicase whose orthologs, DDX5 and DDX17 in humans and Dbp2p in yeast, are implicated in various processes including transcriptional regulation, elongation, and termination, 23 ribosome biogenesis, and RNA decay. Although prd­-1smutantssiois an ATP-dependent RNA helicase, member of a sub-family display a long period (~25 hrs) circadian developmental cycle, they interestingly display a wild type period when the core circadian oscillator is tracked using a frq-luciferase transcriptional fusion under conditions of limiting nutritional carbon; the core oscillator runs with a long period under glucose-sufficient conditions. Furthermore PRD-1 clearly impacts the circadian oscillator and is not only part of a metabolic oscillator ancillary to the core clock. PRD-1 is an essential protein and its expression is neither light-regulated nor clock-regulated. However, it is transiently induced by glucose; in the presence of sufficient glucose PRD-1 is in the nucleus until glucose runs out which elicits its disappearance from the nucleus. Because circadian period length is carbon concentration-dependent, prd­-1 may be formally viewed as clock mutant with defective nutritional compensation of circadian period length.

Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
AC05-76RL01830
OSTI ID:
1237805
Report Number(s):
PNNL-SA-114898; KP1704020
Journal Information:
Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 51; ISSN 0027-8424
Publisher:
National Academy of Sciences, Washington, DC (United States)Copyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 19 works
Citation information provided by
Web of Science

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Cited By (6)

Developmental Dynamics of Long Noncoding RNA Expression during Sexual Fruiting Body Formation in Fusarium graminearum journal August 2018
Sexual stage-induced long noncoding RNAs in the filamentous fungus Fusarium graminearum preprint February 2018
The RNA Helicase BELLE Is Involved in Circadian Rhythmicity and in Transposons Regulation in Drosophila melanogaster journal February 2019
A component of the TOR (Target Of Rapamycin) nutrient-sensing pathway plays a role in circadian rhythmicity in Neurospora crassa journal June 2018
Circadian rhythms, metabolic oscillators, and the target of rapamycin (TOR) pathway: the Neurospora connection journal October 2018
Experimental and Mathematical Analyses Relating Circadian Period and Phase of Entrainment inNeurospora crassa journal November 2017

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59 BASIC BIOLOGICAL SCIENCES
circadian
FRQ
RNA helicase
DDX5
Dbp2p