A crucial role for dynamic expression of components encoding the negative arm of the circadian clock

Wang, Bin; Zhou, Xiaoying; Kettenbach, Arminja N.; Mitchell, Hugh D.; Markillie, Lye Meng; Loros, Jennifer J.; Dunlap, Jay C.

doi:10.1038/s41467-023-38817-7

A crucial role for dynamic expression of components encoding the negative arm of the circadian clock

Journal Article · Thu Jun 08 00:00:00 EDT 2023 · Nature Communications

DOI:https://doi.org/10.1038/s41467-023-38817-7· OSTI ID:1986477

^[1]; Zhou, Xiaoying ^[1]; ^[1]; Mitchell, Hugh D. ^[2]; ^[2]; Loros, Jennifer J. ^[1]; ^[1]

Dartmouth College, Hanover, NH (United States). Geisel School of Medicine
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

In the Neurospora circadian system, the White Collar Complex (WCC) drives expression of the principal circadian negative arm component frequency (frq). FRQ interacts with FRH (FRQ-interacting RNA helicase) and CKI, forming a stable complex that represses its own expression by inhibiting WCC. In this study, a genetic screen identified a gene, designated as brd-8, that encodes a conserved auxiliary subunit of the NuA4 histone acetylation complex. Loss of brd-8 reduces H4 acetylation and RNA polymerase (Pol) II occupancy at frq and other known circadian genes, and leads to a long circadian period, delayed phase, and defective overt circadian output at some temperatures. In addition to strongly associating with the NuA4 histone acetyltransferase complex, BRD-8 is also found complexed with the transcription elongation regulator BYE-1. Expression of brd-8, bye-1, histone h2a.z, and several NuA4 subunits is controlled by the circadian clock, indicating that the molecular clock both regulates the basic chromatin status and is regulated by changes in chromatin. Taken together, our data identify auxiliary elements of the fungal NuA4 complex having homology to mammalian components, which along with conventional NuA4 subunits, are required for timely and dynamic frq expression and thereby a normal and persistent circadian rhythm.

View Accepted Manuscript (DOE)

Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-76RL01830

OSTI ID:: 1986477

Report Number(s):: PNNL-SA-176537

Journal Information:: Nature Communications, Journal Name: Nature Communications Journal Issue: 1 Vol. 14; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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