Isolation of an insulin-like growth factor II cDNA with a unique 5 prime untranslated region from human placenta

Journal Article · Tue Mar 01 00:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.6.1947· OSTI ID:7118310

Shen, Shujane; Daimon, Makoto; Wang, Chunyeh; Ilan, J ^[1]; Jansen, M ^[2]

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1. Case Western Reserve Univ., Cleveland, OH (USA)
2. State Univ. of Utrecht (Netherlands)

Human insulin-like growth factor II (IGF-II) cDNA from a placental library was isolated and sequenced. The 5{prime} untranslated region (5{prime}-UTR) sequence of this cDNA differs completely from that of adult human liver and has considerable base sequence identity to the same region of an IGF-II cDNA of a rat liver cell line, BRL-3A. Human placental poly(A){sup +} RNA was probed with either the 5{prime}-UTR of the isolated human placental IGF-II cDNA or the 5{prime}-UTR of the IGF-II cDNA obtained from adult human liver. No transcripts were detected by using the 5{prime}-UTR of the adult liver IGF-II as the probe. In contrast, three transcripts of 6.0, 3.2, and 2.2 kilobases were detected by using the 5{prime}-UTR of the placental IGF-II cDNA as the probe or the probe from the coding sequence. A fourth IGF-II transcript of 4.9 kilobases presumably containing a 5{prime}-UTR consisting of a base sequence dissimilar to that of either IGF-II 5{prime}-UTR was apparent. Therefore, IGF-II transcripts detected may be products of alternative splicing as their 5{prime}-UTR sequence is contained within the human IGF-II gene or they may be a consequence of alternative promoter utilization in placenta.

• OSTI ID: 7118310
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:6; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FETUSES
GROWTH
GROWTH FACTORS
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA SEQUENCING
INSULIN
LIVER
PHOSPHORUS 32
PLACENTA
TRANSCRIPTION
WOMEN
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DNA
FEMALES
FETAL MEMBRANES
GLANDS
HORMONES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MEMBRANES
MITOGENS
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques