Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Tissue-specific expression of insulin-like growth factor II mRNAs with distinct 5' untranslated regions

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ; ;
The authors have used RNA from human hypothalamus as template for the production of cDNAs encoding insulin-like growth factor II (IGF-II). The prohormone coding sequence of brain IGF-II RNA is identical to that found in liver; however, the 5' untranslated sequence of the brain cDNA has no homology to the 5' untranslated sequence of the previously reported liver cDNAs. By using hybridization to specific probes as well as a method based on the properties of RNase H, they found that the human IGF-II gene has at least three exons that encode alternative 5' untranslated regions and that are expressed in a tissue-specific manner. A probe specific to the brain cDNA 5' untranslated region hybridizes to a 6.0-kilobase transcript present in placenta, hypothalamus, adrenal gland, kidney, Wilms tumor, and a pheochromocytoma. The 5' untranslated sequence of the brain cDNA does not hybridize to a 5.3-kilobase transcript found in liver or to a 5.0-kb transcript found in pheochromocytoma. By using RNase H to specifically fragment the IGF-II transcripts into 3' and 5' fragments, they found that the RNAs vary in size due to differences in the 5' end but not the 3' end.
Research Organization:
Univ. of Zurich (Switzerland)
OSTI ID:
7242204
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:18; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

Isolation of an insulin-like growth factor II cDNA with a unique 5 prime untranslated region from human placenta
Journal Article · Mon Feb 29 23:00:00 EST 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA) · OSTI ID:7118310
Different mRNAs code for dopa decarboxylase in tissues of neuronal and nonneuronal origin
Journal Article · Thu Mar 14 23:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States) · OSTI ID:5079079
Somatomedin-C/insulin-like growth factor-I and Insulin-like growth factor-II mRNAs in rate fetal and adult tissues
Journal Article · Tue Nov 04 23:00:00 EST 1986 · J. Biol. Chem.; (United States) · OSTI ID:7093617

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
DNA SEQUENCING
ENZYMES
ESTERASES
FETAL MEMBRANES
GROWTH FACTORS
HORMONES
HYBRIDIZATION
HYDROLASES
HYPOTHALAMUS
INSULIN
ISOTOPES
KIDNEYS
LIGHT NUCLEI
MAMMALS
MAN
MEMBRANES
MESSENGER-RNA
MITOGENS
NEOPLASMS
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHOSPHODIESTERASES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLACENTA
PRIMATES
PROTEINS
RADIOISOTOPES
RECOMBINANT DNA
RNA
RNA-ASE
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
VERTEBRATES