Molecular cloning and nucleotide sequence of cDNA for human liver arginase

Journal Article · Thu Jan 01 04:00:00 EST 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.2.412· OSTI ID:6022635

Haraguchi, Y; Takiguchi, M; Amaya, Y; Kawamoto, S; Matsuda, I; Mori, M

Arginase (EC3.5.3.1) catalyzes the last step of the urea cycle in the liver of ureotelic animals. Inherited deficiency of the enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. To facilitate investigation of the enzyme and gene structures and to elucidate the nature of the mutation in argininemia, the authors isolated cDNA clones for human liver arginase. Oligo(dT)-primed and random primer human liver cDNA libraries in lambda gt11 were screened using isolated rat arginase cDNA as a probe. Two of the positive clones, designated lambda hARG6 and lambda hARG109, contained an overlapping cDNA sequence with an open reading frame encoding a polypeptide of 322 amino acid residues (predicted M/sub r/, 34,732), a 5'-untranslated sequence of 56 base pairs, a 3'-untranslated sequence of 423 base pairs, and a poly(A) segment. Arginase activity was detected in Escherichia coli cells transformed with the plasmid carrying lambda hARG6 cDNA insert. RNA gel blot analysis of human liver RNA showed a single mRNA of 1.6 kilobases. The predicted amino acid sequence of human liver arginase is 87% and 41% identical with those of the rat liver and yeast enzymes, respectively. There are several highly conserved segments among the human, rat, and yeast enzymes.

• Research Organization: Kumamoto Univ. Medical School, Japan
• OSTI ID: 6022635
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:2; ISSN PNASA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMIDASES
AMIDES
AMINO ACID SEQUENCE
ANIMALS
ARGINASE
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL PATHWAYS
BODY
CARBONIC ACID DERIVATIVES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
DNA
DNA SEQUENCING
ENZYMES
ESCHERICHIA COLI
GENETIC MAPPING
GLANDS
HEREDITARY DISEASES
HYDROLASES
ISOTOPES
LIGHT NUCLEI
LIVER
MAMMALS
MAN
MAPPING
MESSENGER-RNA
MICROORGANISMS
MOLECULAR STRUCTURE
NON-PEPTIDE C-N HYDROLASES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
RATS
RECOMBINANT DNA
RNA
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
UREA
VERTEBRATES